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OfflineMetacanna
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Registered: 01/22/17
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How to trigger pinning. PF jars into bulk in coco
    #25003987 - 02/18/18 03:48 PM (6 years, 1 month ago)

Hey,

I inoculated 1/2 gallon of sterilized 70% coco 30% verm sub with one 1/4 pint BRF jar (15% spawn rate), took 4 weeks to achieve full colonization.
Then I put it in fruiting conditions, with lots of FAE and misting, however after 5 days I had no pins and the mycelium on the top started to dry. So I took it back to incubation conditions, cased with 1/2" of coco/verm, misted really well and waited to see if the mycelium would recover. Indeed, after 10 days the mycelium is looking better and is colonizing the casing. My plan was to put it into fruiting again as soon as first pins showed up, however 10 days have passed the mycelium keep expanding but no sign of fruits. I poked a few holes on the plastic film I'm using to cover the substrate, so there's some air exchange but not enough to dry the sub, I suppose allowing some air exchange can help to initiate fruiting.

Should I wait a bit longer until I have pins or put it into fruiting conditions and try to trigger fruiting with FAE/light?

I guess the strain I have is far from the original vigor, seems slow and weak but have fruited before in PF jars. I'm incubating at 70F and fruiting at 60F, these temperatures are slowing the process, however 6 weeks have passed since inoculation and I have no pins so far. Should I just be patient or change something?

Thanks

Edited by Metacanna (03/04/18 03:08 PM)

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Invisibleimpatientguy
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Metacanna]
    #25003994 - 02/18/18 03:53 PM (6 years, 1 month ago)

Go ahead and start fruiting them:thumbup:


I've seen posts by people saying they put their bulk substrates into fruiting conditions right after mixing the spawn in.


--------------------
Super clean spore printing method: https://www.shroomery.org/forums/showflat.php/Number/5276177


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Invisiblenube424
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Re: How to trigger pinning. PF jars into bulk in coco [Re: impatientguy]
    #25004975 - 02/19/18 05:53 AM (6 years, 1 month ago)

Yea fruit it. U could cut bigger holes in the plastic if u wanna initiate pinning b4 putting in the fc. But... U can just put it in there. Same thing. Itll pin, everything sounds right on time

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OfflineMetacanna
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Re: How to trigger pinning. PF jars into bulk in coco [Re: nube424]
    #25006562 - 02/19/18 07:48 PM (6 years, 1 month ago)

Thanks.
I find it weird that it is taking so long to pin. Anyway this week it will go into the FC no matter what.

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InvisibleMrRabbit-HMR
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Metacanna]
    #25007963 - 02/20/18 12:32 PM (6 years, 1 month ago)

I think I can see knots in your photo though hard to tell. Put it into fruiting conditions, should be fine. Keep us posted...

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OfflineMetacanna
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Re: How to trigger pinning. PF jars into bulk in coco [Re: MrRabbit-HMR]
    #25009907 - 02/21/18 06:42 AM (6 years, 1 month ago)

Today I had a few pins so finally it's again in the FC hoping for decent fruits.

I noticed a few dozen pins on the sides of the container too, which I should have predicted. I'm trying to fix this with tape but I guess the yeild is already compromised...

Edited by Metacanna (03/04/18 03:09 PM)

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OfflineTurd_Burglar
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Metacanna]
    #25009925 - 02/21/18 06:56 AM (6 years, 1 month ago)

More spawn would help the colonization times for sure, I do the same PF tek to bulk 2 parts coir/ one part verm. Try giving them some light fanning here and there and some light. Also Ive been adding gypsum to my bulk subs too, seems to speed things up I think


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....................:holyshit:....................

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Invisiblenube424
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Turd_Burglar]
    #25010653 - 02/21/18 01:45 PM (6 years, 1 month ago)

Yea use a high spawn ratio. With cakes u can even go over 1:1.    1.5:1 would probly be great with cakes.

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OfflineMetacanna
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Re: How to trigger pinning. PF jars into bulk in coco [Re: nube424]
    #25032146 - 03/01/18 03:18 PM (6 years, 28 days ago)

11 days after start pinning:


Fruiting temps have been ranging 60F-63F.
My hygrometer sucks, so I have no idea what humidity am I working with. I mist once a day enough to keep the top layer moist, I avoid misting directly on the mushrooms. What intrigues me is that fuzzy stems indicates me I might have abused on misting, on the other hand the stems looks dry and some caps cracked which indicates the opposite. What do you say? Inconsistent humidity or genetics?

I will be harvesting in 2 days I guess. I would be happy with 5g dry  :wow:

Edited by Metacanna (03/04/18 03:09 PM)

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InvisibleMateja
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Metacanna]
    #25032187 - 03/01/18 03:34 PM (6 years, 28 days ago)

I don´t claim to know what that fuzz is but this is what I would speculate:

You mentioned that your substrate dries out reasonably quickly so that would not indicate lack of FAE.
The word on the street is of course that white fuzz on the stem is due to lack of FAE, but I´m speculating if
there could possibly be two kinds of white fuzz on the stems.

From my observations so far I have notice two kinds of white fuzz of the stems.
One being clear white soft, tomentose mycelium running pretty far up the stem (which I believe indicates lack of FAE)

And the other being a more white/yellowish spikey, pointy fuzz, concentrated near the base (which I believe is due to some bacterial infection)

To me the fuzz on your fruits looks more like the latter to me..

I´m sure someone will give you a better explanation of what´s going on. GL


--------------------
Cakes inside Water Tub

Edited by Mateja (03/01/18 03:35 PM)

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Invisiblenube424
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Re: How to trigger pinning. PF jars into bulk in coco [Re: Mateja]
    #25032208 - 03/01/18 03:47 PM (6 years, 28 days ago)

Quote:

Mateah said:
I don´t claim to know what that fuzz is but this is what I would speculate:

You mentioned that your substrate dries out reasonably quickly so that would not indicate lack of FAE.
The word on the street is of course that white fuzz on the stem is due to lack of FAE, but I´m speculating if
there could possibly be two kinds of white fuzz on the stems.

From my observations so far I have notice two kinds of white fuzz of the stems.
One being clear white soft, tomentose mycelium running pretty far up the stem (which I believe indicates lack of FAE)

And the other being a more white/yellowish spikey, pointy fuzz, concentrated near the base (which I believe is due to some bacterial infection)

To me the fuzz on your fruits looks more like the latter to me..

I´m sure someone will give you a better explanation of what´s going on. GL



Yup. But the spikey stuff is from standing water. Which would also likely be bacteria . adter a dunk the pins get spikey.

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InvisiblemushboyMDiscord
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Re: How to trigger pinning. PF jars into bulk in coco [Re: nube424]
    #25032212 - 03/01/18 03:51 PM (6 years, 28 days ago)

that sub and those shrooms look way to wet. either your rh is way to high or your going mister crazy. or you sub is soaked.

dry the out. see better fruits.:thumbup:

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Invisiblenube424
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Re: How to trigger pinning. PF jars into bulk in coco [Re: mushboy]
    #25032274 - 03/01/18 04:18 PM (6 years, 28 days ago)

Leave the lid off till its drier. Or leave the lid on and stop misting till u see no water

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OfflinecronicrFacebook
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Re: How to trigger pinning. PF jars into bulk in coco [Re: mushboy]
    #25032377 - 03/01/18 10:14 PM (6 years, 28 days ago)

Quote:

mushboy said:
that sub and those shrooms look way to wet. either your rh is way to high or your going mister crazy. or you sub is soaked.

dry the out. see better fruits.:thumbup:



No such thing as too high of an rh


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OfflineMetacanna
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Re: How to trigger pinning. PF jars into bulk in coco [Re: cronicr]
    #25032911 - 03/02/18 07:30 AM (6 years, 28 days ago)

Thanks for your comments and suggestions, notes taken.
I just harvest today the first flush. From my observation on this grow and based on your suggestions, for the second flush I will be more moderate when misting, more on the walls and lid, less on the substrate so the rh is high and the sub is not soggy.

First time on bulk, I'm happy  :cookiemonster:

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