|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
doomshroomz
holy roller
Registered: 11/29/17
Posts: 26
Last seen: 6 years, 3 months
|
Agar separates from nutrients after PC
#24815240 - 11/29/17 07:48 PM (6 years, 3 months ago) |
|
|
hey there okay this is my first post and i created an account because turns out i have a few very specific questions...
SO
I'm using dog food agar (1000 mL spring water, 40g dog food, 20g agar, and 2g yeast). I load them into half pint mason jars with A hole or two at the top stuffed with polyfil for inoculation. I put them to PC at 12psi for 45 minutes, wrapped in foil to prevent any moisture in the polyfil. When I took it out of the pressure cooker the nutrients had separated entirely from the agar (agar on top, dog food at the bottom).
I'm thinking this may have happened because at one point i had to switch pressure valves, and so took f the original 10 psi pressure valve to switch it for twelve but it was quite a moment before i could get it on so my PC was letting out full steam for a minute after already at pressure.
Was this it? Is there another reason this may have happened? Any help from any experienced cultivators would be of tremendous help.
|
Snazz
Polymath
Registered: 11/24/15
Posts: 1,584
Loc: Canada
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24815242 - 11/29/17 07:50 PM (6 years, 3 months ago) |
|
|
Was the agar set? Or still warm liquid.
|
doomshroomz
holy roller
Registered: 11/29/17
Posts: 26
Last seen: 6 years, 3 months
|
Re: Agar separates from nutrients after PC [Re: Snazz]
#24815250 - 11/29/17 07:55 PM (6 years, 3 months ago) |
|
|
I took the agar out of the PC once it was pretty much set. it may have been a little fluid but definitely not liquid. They seemed set.
|
Snazz
Polymath
Registered: 11/24/15
Posts: 1,584
Loc: Canada
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24815286 - 11/29/17 08:07 PM (6 years, 3 months ago) |
|
|
Maybe pull them sooner and give a swirl.
Although agar typically isn't very deep. How full are these half pints? I'd say the nutrition is enough for what dissolved.
|
doomshroomz
holy roller
Registered: 11/29/17
Posts: 26
Last seen: 6 years, 3 months
|
Re: Agar separates from nutrients after PC [Re: Snazz]
#24815299 - 11/29/17 08:15 PM (6 years, 3 months ago) |
|
|
Do you think it was too much PC time?
I'm thinking there was a release of pressure when i did that valve thing that may have caused it.
The jars are about 5/8 to 3/4 of the way full.
I did this because i thought my syringe would have to reach into it but i recently learned that i can just put drops on the surface.
Also its been about 6 days and nothing (not even bacteria has grown) which makes me think one of two things is true:
Either i PC'd it for too long and the nutrients caramelized/complex sugars were degrade (not extremely likely) OR that the agar being all the way at the bottom (maybe 2-3in under the surface) makes it impossible for anything on the surface to be fed.
|
Snazz
Polymath
Registered: 11/24/15
Posts: 1,584
Loc: Canada
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24815356 - 11/29/17 08:36 PM (6 years, 3 months ago) |
|
|
I'd go with a half inch of liquid.
Pain to work with high glass sides tho.
Most of us use 4oz containers.
|
hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,056
Loc: ation is turned off.
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24815374 - 11/29/17 08:43 PM (6 years, 3 months ago) |
|
|
Too much dog food. Only half the amount you used is required for 1000ml of water.
|
doomshroomz
holy roller
Registered: 11/29/17
Posts: 26
Last seen: 6 years, 3 months
|
Re: Agar separates from nutrients after PC [Re: hamloaf]
#24815417 - 11/29/17 09:01 PM (6 years, 3 months ago) |
|
|
Quote:
hamloaf said: Too much dog food. Only half the amount you used is required for 1000ml of water.
I read on the forums that you could use anywhere from 20-40 g. IIRC something about nutrients can be anywhere from 2-4% or 3-5% or something.
Have you heard of this/what do you think about this?
Edited by doomshroomz (11/29/17 09:01 PM)
|
Mycolorado
Hobbyist
Registered: 07/23/16
Posts: 8,567
Loc: Interdimensional Bootcamp
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24815478 - 11/29/17 09:37 PM (6 years, 3 months ago) |
|
|
Dog food has a tendency to settle and depending on the type/brand/quality, some may settle more than others. I’d say it’s better used in a pour method so you can swirl to ensure it’s good and suspended when pouring each plate.
|
hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,056
Loc: ation is turned off.
|
Re: Agar separates from nutrients after PC [Re: doomshroomz]
#24816035 - 11/30/17 06:16 AM (6 years, 3 months ago) |
|
|
Quote:
doomshroomz said:
Quote:
hamloaf said: Too much dog food. Only half the amount you used is required for 1000ml of water.
I read on the forums that you could use anywhere from 20-40 g. IIRC something about nutrients can be anywhere from 2-4% or 3-5% or something.
Have you heard of this/what do you think about this?
I wouldn't use so much according to the recipes I've read. Mycolorado brings up a good point.
|
Munchauzen
Registered: 06/22/11
Posts: 14,343
|
Re: Agar separates from nutrients after PC [Re: hamloaf] 1
#24816051 - 11/30/17 06:30 AM (6 years, 3 months ago) |
|
|
MEA is the best recipe for no pours as everything is fully dissolved very easily
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 547 topic views. 16 members, 94 guests and 135 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|