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Offlineultramanjay
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Preparing Agar Jars with Micropore tape only as Filter.
    #24019006 - 01/18/17 01:50 AM (7 years, 1 month ago)

Hey guys. I am gonna give myself a go at doing Agar. Im a complete newb in this hobby. So please give me a chance to be stupid. I failed at pf tek (probably because of a contaminated print i got from wild shrooms that i found). So I decided to learn agar. The problem is these are the only materials that i have (As far as preparing agar is concerned) :

-Pressure Cooker
-Quart Jars
-Syringes
-Micropore tape
-Oatmeal agar ingredients
I dont have access to tyvek and polyfill.

What I am planning to do is:
1. Drill 1 hole per jar lid
2. Cook the Agar
3. Pour into quart jars
4. Close the lids (Tape the lid holes with micropore) then cover them with foil
5. Pressure cook the jars and let cool
6. Inject spores using syringes with my probably contaminated prints.
7. Let them colonize then do transfers.

Will this method be viable? Any tips on how I can pull this one off will be so f***in appreciated. Thanks!


--------------------
“In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.”

― Richard Preston

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Offlinephunkeej
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: ultramanjay]
    #24019058 - 01/18/17 02:42 AM (7 years, 1 month ago)

If your not in the process right now wait and grab some free tyvek envelopes from your local post office..i only used tape on jars for years without any problems from it that wouldn't have existed anyway..you can even have free shipping materials shipped to your house...

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Offlineultramanjay
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: phunkeej]
    #24019064 - 01/18/17 02:48 AM (7 years, 1 month ago)

Thats not possible. As I have said, I have no access to Tyvek. Forever.


--------------------
“In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.”

― Richard Preston

Extras: Filter Print Post Top
Offlinephunkeej
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: ultramanjay]
    #24019069 - 01/18/17 02:53 AM (7 years, 1 month ago)

well anyways, i had no issues i had probably a 80-85% success rate over the first 8 yrs or so not using any filter layer except dry vermiculite..i'm sure you'll be fine...good luck and peace...

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InvisiblebodhisattaMDiscordReddit
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: phunkeej] * 1
    #24019367 - 01/18/17 08:45 AM (7 years, 1 month ago)

https://www.shroomery.org/forums/showflat.php/Number/19208976

Here's the most popular agar Tek on the site. Its hard not to accidentally find it if you type agar Tek in the search engine...

Advanced mycology should be closed to people with less than 5000 posts and 3 years here.

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InvisiblePrisoner#1
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Re: Preparing Agar Jars with Micropore tape only as Filter. (moved) [Re: ultramanjay]
    #24019513 - 01/18/17 09:58 AM (7 years, 1 month ago)

This thread was moved from Advanced Mycology.

Reason:
more appropriate forum

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InvisibleAndyHinton


Registered: 12/05/16
Posts: 434
Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: bodhisatta]
    #24019811 - 01/18/17 12:13 PM (7 years, 1 month ago)

Update: Reached 50 posts.


--------------------

Edited by AndyHinton (01/26/17 09:35 AM)

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InvisiblemushboyMDiscord
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: AndyHinton]
    #24019828 - 01/18/17 12:24 PM (7 years, 1 month ago)

Quote:

bodhisatta said:
Advanced mycology should be closed to people with less than 5000 posts and 3 years here.




:uhno:

some of us do not post in advance forum because we know our bounds.

advanced forum SHOULD have some kind of douche filter but not that obtuse.:thumbdown:
or based off of post stats.(hint* im like 98% cult forum)

ANYWAY.. OP,

trying to cut agar out of a quart jar is not going to be practical.
i use tape and tyvek. you can survive without the tyvek but i like being careful.


everything else about agar is incredibly straight forward so its hard to fuck up.

grow a culture.
cut a clean, healthly leading edge piece and drop on another plate/perti
grow out, repeat if needed. use wedge as inoculate in numerous ways.

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Offlineultramanjay
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: AndyHinton]
    #24021069 - 01/18/17 07:13 PM (7 years, 1 month ago)

Quote:

AndyHinton said:
Quote:

ultramanjay said:
Thats not possible. As I have said, I have no access to Tyvek. Forever.



Did you get banned from the post office or something?





We live in different countries man. In the post office here, you dont get that for free.

Anyways, Thanks for the tips from all of you guys! And for posting on the wrong forum, Im so sorry people. As I have said, im a complete newb so this will be a part of my experience. Im a failure :frown: But thanks again!


--------------------
“In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.”

― Richard Preston

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InvisiblemushboyMDiscord
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: ultramanjay]
    #24021207 - 01/18/17 07:56 PM (7 years, 1 month ago)

in soviet post office, you are the parcel!

try a hardware store... or a store that sells paint. tyvek painters suits!!

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InvisiblebodhisattaMDiscordReddit
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: mushboy]
    #24021559 - 01/18/17 10:17 PM (7 years, 1 month ago)

well he has a post office, tyvek just isn't free. so if that can't be afforded chances are neither can the tyvek at the hardware store

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Offlineultramanjay
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: bodhisatta]
    #24021860 - 01/19/17 02:26 AM (7 years, 1 month ago)

Nope. I go in to any hardware store I can find. People here just dont have a use for that sheet. *pun intended* anyways, forget tyvek. ill roll with just micropore. Ill just be extra careful.


--------------------
“In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.”

― Richard Preston

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OfflinePanarchist
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: ultramanjay]
    #24024250 - 01/19/17 09:23 PM (7 years, 1 month ago)

another problem with large jars is going to be that when you inject the spores into the jar from that height they are going to splash all over the surface of the agar which may result in growth that is too disorganized to find any clean transfer sections.


--------------------

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InvisibleFick_Duck
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Re: Preparing Agar Jars with Micropore tape only as Filter. [Re: mushboy]
    #24024319 - 01/19/17 09:47 PM (7 years, 1 month ago)

Quote:

mushboy said:
in soviet post office, you are the parcel!

try a hardware store... or a store that sells paint. tyvek painters suits!!






That made me laugh. In Soviet hardware store, Tyvec sells YOU!!!


--------------------
"To know life you must fuck it in the liver." -Dr. Frankenstein, Andy Warhols Frankenstein

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