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InvisibleStix Russell
AmorphousExperimentalist

Registered: 11/26/99
Posts: 113
Elementary question regarding Liquid Growth Medium
    #2382214 - 02/27/04 07:18 AM (20 years, 1 month ago)

So a spore print was utilized in the creation of liquid growth medium, sterile conditions, the liquid is as clear as crystal, 4% by weight Karo syrup in sterile, filtered water. My problem is that the spores from the spore print that were scraped into the water are floating on the surface of the medium, looking as if the surface tension of the water is holding them there. Is this what is expected? Will this affect germination? When they are hydrated will they sink into the depths of the medium?


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Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 20 hours
Re: Elementary question regarding Liquid Growth Medium [Re: Stix Russell]
    #2382225 - 02/27/04 07:23 AM (20 years, 1 month ago)

Have you tried swirling them around by moving the jar in circular motion?

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InvisibleStix Russell
AmorphousExperimentalist

Registered: 11/26/99
Posts: 113
Re: Elementary question regarding Liquid Growth Medium [Re: Anno]
    #2382233 - 02/27/04 07:31 AM (20 years, 1 month ago)

I have, but not in a vigorous manner in fear that a large number of spores become deposited on the interior of the lid or upper walls of the jar, rendering them devoid of hydration. Should this be a concern?

Thanks for your response, Anno.


--------------------
Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)

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InvisibleMilkVein
unsure
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Registered: 08/27/03
Posts: 2,695
Loc: total bullshit->
Re: Elementary question regarding Liquid Growth Medium [Re: Stix Russell]
    #2383011 - 02/27/04 11:45 AM (20 years, 1 month ago)

just shake a bit...it will be fine.


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I've been first and last
Look at how the time goes past.
But I'm all alone at last.
Rolling home to you.

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