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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Rye jars driving a biologist crazy *DELETED*
#23682142 - 09/27/16 04:03 AM (7 years, 5 months ago) |
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Post deleted by AlmostBlueReason for deletion: not useful
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682175 - 09/27/16 04:43 AM (7 years, 5 months ago) |
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Quote:
i'm a biologist and know lots about mushrooms and microbiology in general.
Quote:
In my opinion someone of my family found the jars, got curious and opened them, i can see no other explaination for these contams!
as someone who has a biology background, this is your first instinct…?
what are they teaching you in school?!?!?
those grains, even before shaking, looking a little off. may have been MS syringe (bacteria) or filter failure (mold)
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
#23682191 - 09/27/16 04:55 AM (7 years, 5 months ago) |
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If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 5 months
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682193 - 09/27/16 04:59 AM (7 years, 5 months ago) |
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Quote:
Inocuole said: If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.
as a biologist who's worked with agar you must know spore syringes are full of crap that's why you used agar.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE Click here for UK trades need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
#23682199 - 09/27/16 05:03 AM (7 years, 5 months ago) |
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Hey nerdy boy keep calm. Looks like you're not the only mushroom grower out there.
I say this because i'm pretty sure my filter system didn't fail as it was STERILE and SUPER TIGHT.
Also my siringe was ordered from the internet, and flame sterilized the needle every time in and out.
I've been out of town for 4 days and when I left it looked super healty that's why i think someone opened it while i was out.
Still you are a 'trusted cultivator' but didn't reply to my simple questions.
If you are on your period just leave my thread, thanks
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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682214 - 09/27/16 05:20 AM (7 years, 5 months ago) |
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Quote:
Inocuole said: If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.
yeah well i wanted to spawn the grain and colonize a big monotub, what's the right way to go about it?
ONLY RELEVANT REPLIES PLEASE, OTHERWISE JUST GO ON PORNHUB YOU'LL FEEL MORE CONFIDENT THEN
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TheLongComma
Ouch My Balls
Registered: 08/07/16
Posts: 60
Last seen: 3 days, 18 hours
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682215 - 09/27/16 05:21 AM (7 years, 5 months ago) |
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Wouldn't matter if your needle was sterile if the spores inside were dirty to begin with.
Also, you said you micropored the hole after inoculation? Does that mean you took the polyfill out to do that?
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682217 - 09/27/16 05:22 AM (7 years, 5 months ago) |
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will I feel as confident at porn hub as you did with your "internet syringes" and"sterile tight" filter?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Rye jars driving a biologist crazy [Re: TheLongComma]
#23682218 - 09/27/16 05:24 AM (7 years, 5 months ago) |
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I guess this guy doesn't want help with his bacterial jar.. not that there's any helping it. You either use agar to ensure you have a clean culture or you can keep guessing between inoculate or filter fail until something gives... In case BLs sarcasm wasn't laid on thick enough "internet syringes" are just as likely to harbor bacteria as anything else.
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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
#23682238 - 09/27/16 05:59 AM (7 years, 5 months ago) |
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Well, I felt like doing agar was not necessary as the syringe provider (inner visions) said the spore syringe was contam free, and i had no interest in selecting genetics, and little time to do the whole thing.
Opening a sterile jar to insert an agar slice isn't safer than inoculating from a hole if you assume your syringes are contam-free!
Never took out the polyfill, taped the outside after inoculation just to be safer.
Your sarcasm is totally pointless and just shows me how twisted and sick you guys are. Just give your opinion, not stupid pointless critiques just because you feel 'experts'.
I previously realized a 3d carboard puzzle model of an ancient building, sterilized, filled it with substrate and spawn, let it colonize and managed to fruit beautiful mushrooms from the 'windows' of the building. and i was 18yo at the time.
As I said, you are not the only mushroom growers out there. It's pretty easy actually! So stop acting like pussies and tell me if it's worth fruiting the unshaken jars with a casing layer or just as cakes rolled in verm, which was the ONLY question i've asked so far.
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nuentoter
conduit
Registered: 09/17/08
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682249 - 09/27/16 06:06 AM (7 years, 5 months ago) |
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Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.
-------------------- The geometry of us is no chance. We are antennae, we are tuning forks, we are receiver and transmitters of all energy. We are more than we know. - @entheolove "I found I could say things with color and shapes that I couldn't say any other way - things I had no words for" - Georgia O'Keefe I think the word is vagina
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
#23682260 - 09/27/16 06:19 AM (7 years, 5 months ago) |
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Oh dear this is going well, this all smells of b.s. any biologist knows the basics and internet syringes are not clean Why don't you stop being a pussy and utfse and figure it out yourself
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE Click here for UK trades need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
Edited by mrmazdarx9 (09/27/16 06:24 AM)
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
#23682270 - 09/27/16 06:28 AM (7 years, 5 months ago) |
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just throw some coir on top, keep it in the jar, and see if it works for crying out loud.
jesus christ, if u made fucking 3D oyster puzzles (when u were 18 y/o no less ), i'd think u'd have at least SOME idea of how to proceed with those jars.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
#23682273 - 09/27/16 06:30 AM (7 years, 5 months ago) |
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Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great?
Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.
This forum's a real bitch!
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
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Last seen: 1 year, 5 months
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682276 - 09/27/16 06:32 AM (7 years, 5 months ago) |
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Bye then
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE Click here for UK trades need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
#23682277 - 09/27/16 06:32 AM (7 years, 5 months ago) |
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"Your not the only mushroom grower, it's not that hard!!!! Now please help me!!!'
They already did. Start over: use agar, make a few transfers and innoculations your jar with clean spawn. First pull the stick from yer bunghole
You haven't gotten a single fruit so how can you say the other stuff is going great
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: filthyknees]
#23682281 - 09/27/16 06:34 AM (7 years, 5 months ago) |
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yes, we're the worst. have a nice day
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
#23682298 - 09/27/16 06:44 AM (7 years, 5 months ago) |
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Quote:
nuentoter said: Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.
I wouldn't bother trying to save that mycelium in particular when he could just start from spores again and maybe have a better chance of isolating something clean straight away. If mold gets a chance to interlace with a culture, then it's difficult/near impossible to separate them. Bacteria's a little easier but still a pain.
Quote:
AlmostBlue said: Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great?
Made LC with spores, thinks it's doing great.
I figured you could take it bluntly since you were a biologist but I guess you're one of those experts... the ones that already know everything and dismiss good advice while flaunting their mistakes as if they're marks of success. I didn't anticipate you getting your feelings hurt though. Quite unfortunate.
If you want to know why people were short with you to begin with, it's because you said this
Quote:
Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.
And then proceeded to list off one noob thing after another, like you may as well have never heard of mushrooms at all prior to this. A proper grow goes a lot smoother with a proper mindset, and I don't think anybody here is seeing that you have that..
Edited by Inocuole (09/27/16 06:49 AM)
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682308 - 09/27/16 06:51 AM (7 years, 5 months ago) |
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everything 100%
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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AlmostBlue
Stranger
Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
#23682310 - 09/27/16 06:52 AM (7 years, 5 months ago) |
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Of course i have some ideas (...) actually i'm asking what to do cause here i can find people with more experience with these techniques and if some real expert says i'm just gonna lose my time i'd toss everything away and go fuck myself.
My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!
I'll try anyway, some cased with coir and some rolled in verm and set in fruiting chamber
What a bunch of psychos i was just asking a REALLY SIMPLE question, and still the most of you just wrote something else to make fun of me (how poor you are), not really reading my first post about trying to save unshaken jars rather than starting the whole process with new grains due to no time. Read-->Understand-->Reply is a hard protocol nowdays
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