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mark2000
journeyman
Registered: 11/02/03
Posts: 61
Last seen: 10 years, 11 months
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What in the hell just happened!!lol
#2284977 - 01/29/04 06:35 PM (20 years, 2 months ago) |
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Alright my buds story from 10 mins ago,
I built my first glovebox, for the first ever innoc. Now i used a bag, inside a box, with gloves holes and duct tape. I sterilzed the needle, Lysol'd everything down and started. I did the jars one by one. After each i opened the box and swapped, then hosed down again. Somehow the lysol made the tape not stick and before i knew it my hands were right in there, i ended up ripping the hanging gloves out half way and began using spares i just stuck right threw. It was almost laughable at this point...Basically do you guys think i'll get away with this??? ...6 jars
Damnation
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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Re: What in the hell just happened!!lol [Re: mark2000]
#2284993 - 01/29/04 06:41 PM (20 years, 2 months ago) |
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you where using the glove box for innoculating sterilized jars? if so they should be fine, ive done open air innoculations of grain, and grain to grain transfers too, both methods worked nicely.
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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Che_Night_Soil
Koinoniphobic
Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
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Re: What in the hell just happened!!lol [Re: mark2000]
#2284996 - 01/29/04 06:41 PM (20 years, 2 months ago) |
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Yeah, as clean as you were, and with (I'm assuming) a verm barrier in pf jars or polyfill/tyvek for grain jars, they should be fine. Did you wipe them down before putting them in the incubator?
-------------------- if(human==autoPart){ for(i=1;i<infinity;i++){ getBorn(mind,soul); getEdu(mind,soul); getJob(mind,soul); retire(mind,soul); die(mind,soul); } }
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TheDarkSideof_Paco
Seeker
Registered: 11/10/03
Posts: 219
Loc: My basement
Last seen: 17 years, 5 months
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Re: What in the hell just happened!!lol [Re: mark2000]
#2285505 - 01/29/04 09:37 PM (20 years, 2 months ago) |
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Damn alot more sterile than my first attempt, I had good success too! I think its gonna work out for you.
I vote best newbie question of the week! Glad to see you did some reading
-------------------- "In order to rally people, governments need enemies. They want us to be afraid, to hate, so we will rally behind them. And if they do not have a real enemy, they will invent one in order to mobilize us." Thich Nhat Hanh
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OldSpice
Geritol Breath...
Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
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Post deleted by Administrator [Re: mark2000]
#2285582 - 01/29/04 09:55 PM (20 years, 2 months ago) |
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-------------------- So hard to be ....WDWGFH? Texas is humongus compared to France Our Gair, who art in Texas, Paw Paw be thy Name.... My friends are thirsty You never see a motorcycle parked outside a Psychiatrist office
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mark2000
journeyman
Registered: 11/02/03
Posts: 61
Last seen: 10 years, 11 months
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Re: What in the hell just happened!!lol [Re: OldSpice]
#2285878 - 01/29/04 11:44 PM (20 years, 2 months ago) |
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Ah great to hear back,
Yeah the research has def. been done. By the way its popcorn/polyfil, cubes. In at an 86 degree tub in tub now
I'll keep you updated on his work, as i'm inspired by the popcorn stories
Note: soaked for at least 72 hours and the water was changed 3 times, didnt have a choice don't ask.
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m0rb
mushroom mobster
Registered: 05/07/03
Posts: 184
Loc: New England
Last seen: 19 years, 8 months
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Re: What in the hell just happened!!lol [Re: mark2000]
#2286109 - 01/30/04 01:50 AM (20 years, 2 months ago) |
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Hello Mark,
Your jars will generate some heat of their own, because they are alive you know! I would lower your incubator temps to around 84 which seems to work best for most. When you go with 86 degrees or higher, jars somtimes dry out too quick. Best of luck!
-m0rb-
-------------------- "The business of America is business," - Calvin Coolidge
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