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 AgarStudent
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Registered: 01/25/15
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 Tampensis on Agar 
#21173593 - 01/25/15 04:48 AM (9 years, 2 months ago) |
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Hi, Very much a newbie to this but this is my third ($$$!!) attempt at getting this right. In a sterile environment I have transferred spores to agar and the agar is well hydrated, as this came from the good part of a DIY 20ml spore syringe.
There is still a fair bit of water floating inside, but does it look like the Tampensis mycelium is colonizing? How long until you think I can transfer this to grain (assuming the white streaks are mycelium)?
Please refer to the attached pic (converted to PDF because I can't upload photos?). Any feedback is appreciated.
Edited by AgarStudent (01/25/15 04:50 AM)
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DredgeMyEyes
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Well, i'm new to agar, just started with it but I can tell you that you should have just put the smallest drop from the syringe possible in the center of the petri and let the spores germinate and run out from there so that you can watch for contams easily while it is colonizing and then, after that, transfer to grain if it was indeed clean. it sounds and looks like maybe you had drown it in spore solution.
Someone else more experienced is going to have to chime in on that pic, I have yet to see anything like that. I have not seen 20ml syringes sold by vendors either, did you make it from a print yourself?
Hope it turns out for ya though man, i'm gonna watch this post to see
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AgarStudent
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Good to hear you are working with agar too. Do you stick with a particular recipe?.. as there are heaps to pick. Nutrient is nutrient right? I boiled up a potato.
Sure, you make a print and soak some water off it. Then you suck the water back into a syringe and presto. You leave that in a fridge to let the spores expand and use it for later. Well at least that is the theory, yet it is yet to work for me this time!
I sucked back a bit of that extra water and fed it to a jar of grain. The rest of that white stuff is still sitting there.
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bw86
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edit sorry
i cant see the pic
Edited by bw86 (01/25/15 07:06 AM)
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PinPornProducer
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Re: Tampensis on Agar [Re: bw86]
#21173785 - 01/25/15 07:11 AM (9 years, 2 months ago) |
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I'm no expert but I'm pretty sure not all agar recipes work for both cubes and exotics.
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DredgeMyEyes
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Re: Tampensis on Agar [Re: bw86]
#21173786 - 01/25/15 07:11 AM (9 years, 2 months ago) |
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Quote:
bw86 said:
edit sorry
i cant see the pic
It's a PDF file at the very top.
OP should reupload the picture as a jpg in the "Pics" section so it is more readily viewed
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DredgeMyEyes
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Quote:
AgarStudent said:
Good to hear you are working with agar too. Do you stick with a particular recipe?.. as there are heaps to pick. Nutrient is nutrient right? I boiled up a potato.
Sure, you make a print and soak some water off it. Then you suck the water back into a syringe and presto. You leave that in a fridge to let the spores expand and use it for later. Well at least that is the theory, yet it is yet to work for me this time!
I sucked back a bit of that extra water and fed it to a jar of grain. The rest of that white stuff is still sitting there.
Frank's Agar Media Journal
Some great recipes and info here from FrankHorrigan
I just used some Agar I got from an asian food market and some light malt extract I already had and then I used
Pasty's Easy Agar Tek
to prepare it and it is working well.
You already had fruited some Tampanensis to make a print?
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AgarStudent
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Registered: 01/25/15
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Oh here is the URL showing the attached agar culture of the Tampanesis. Sorry the spelling was wrong originally!
Does this look like germination? Unfortunately the temperature here has dropped again to 20 degrees or so and optimal growth temperature is up around 25 Celsius.
Do you know if dips in temperature are likely to upset the mycelium too much?
I have used a potato-based agar, based on boiling a potato and adding dextrose, brewers yeast and agar-agar with demineralised water.
Much appreciated, any feedback eagerly anticipated 
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Jeff Vader
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Doesnt look good to me. I have Tampensis on agar at home. Looks nothing like that..
Quote:
AgarStudent said:
I have used a potato-based agar, based on boiling a potato and adding dextrose, brewers yeast and agar-agar with demineralised water.
I am using the same recipe.
Quote:
AgarStudent said:
Does this look like germination? Unfortunately the temperature here has dropped again to 20 degrees or so and optimal growth temperature is up around 25 Celsius.
Do you know if dips in temperature are likely to upset the mycelium too much?
Its been around 22 degree by me, I did spore to agar. Had visible growth in 3 days.
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AgarStudent
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Thanks for the feedback. There are other agar plates that have no sign of activity at all,
but the Tampanesis is showing these streaky white patterns. It has only been three days I guess, fingers crossed.
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Why reinvent the wheel when someone here has already done the science?
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