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InvisiblemagicMerlin
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Registered: 01/26/12
Posts: 617
Loc: Toronto
Blue veins on agar?
    #20759803 - 10/27/14 02:39 PM (9 years, 5 months ago)

I searched and couldn't find anything.

I have some ~1 month agar plates grown 100% that were sealed away in the fridge. Inspecting them they all look really dried out but contam free. 2 of the plates have blue veins running through the myc. Is this a contam or just a result of dried out agar?

Using MEA in pp5s

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OfflinePudgy
Fail-2-Win

Registered: 09/30/14
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Re: Blue veins on agar? [Re: magicMerlin]
    #20759808 - 10/27/14 02:41 PM (9 years, 5 months ago)

pics pics pics pics I'm crazy curious!


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InvisiblemagicMerlin
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Registered: 01/26/12
Posts: 617
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Re: Blue veins on agar? [Re: Pudgy]
    #20759814 - 10/27/14 02:42 PM (9 years, 5 months ago)

Quote:

Pudgy said:
pics pics pics pics I'm crazy curious!




I'm not crazy about posting pictures to here but if this is something unusual I may have to.

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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: Blue veins on agar? [Re: magicMerlin]
    #20759815 - 10/27/14 02:43 PM (9 years, 5 months ago)

yeah thats it drying out


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InvisiblemagicMerlin
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Posts: 617
Loc: Toronto
Re: Blue veins on agar? [Re: cronicr]
    #20759820 - 10/27/14 02:44 PM (9 years, 5 months ago)

Quote:

cronicr said:
yeah thats it drying out




Do you think it could recover on WBS if I tiger dropped it? Or is it too far gone?

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InvisibleInocuole
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Re: Blue veins on agar? [Re: magicMerlin]
    #20759823 - 10/27/14 02:45 PM (9 years, 5 months ago)

Quote:

magicMerlin said:
Quote:

Pudgy said:
pics pics pics pics I'm crazy curious!




I'm not crazy about posting pictures to here but if this is something unusual I may have to.




Please sir, post some pictures.  :cop:


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OfflinecronicrFacebook
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Re: Blue veins on agar? [Re: magicMerlin]
    #20759835 - 10/27/14 02:49 PM (9 years, 5 months ago)

it will more then likely work but the fresher the better


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InvisiblemagicMerlin
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Loc: Toronto
Re: Blue veins on agar? [Re: cronicr]
    #20759840 - 10/27/14 02:51 PM (9 years, 5 months ago)

Mystery solved, thanks cronicr

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OfflineKizzle
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Re: Blue veins on agar? [Re: magicMerlin]
    #20760258 - 10/27/14 04:37 PM (9 years, 5 months ago)

Oh yeah I think I know the "veins" you're talking about. Like what you see when the plates are old and start pinning?


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OfflinePudgy
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Re: Blue veins on agar? [Re: Kizzle]
    #20762906 - 10/28/14 08:21 AM (9 years, 4 months ago)

Quote:

Kizzle said:
Oh yeah I think I know the "veins" you're talking about. Like what you see when the plates are old and start pinning?




Please elaborate.

I just did 6 transfers of my best 3 myc cultures.

M5-2, M7-2 and M8-2 ... M5-2 and M7-2 both had a bluish tinge only viewable from above the plate.

It didn't look at all like contamination, were they about to start pinning or were they suffering from lack of air?


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Invisibleblindingleaf
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Re: Blue veins on agar? [Re: Pudgy]
    #20762962 - 10/28/14 08:48 AM (9 years, 4 months ago)

sometimes, depending on where i put stored cube plates in the fridge, they turn blue if they were frozen for a short time.  usually near the back on top shelf, closest to freezer.  the cell walls burst from freeze/defrost, and bruise blue.
i haven't ever put plates like that to grains, i'd transfer once more to ensure viability, then go to grains.  but if u do try to go direct to grains, post results.


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OfflinePudgy
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Registered: 09/30/14
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Re: Blue veins on agar? [Re: blindingleaf]
    #20763012 - 10/28/14 09:04 AM (9 years, 4 months ago)

Quote:

blindingleaf said:
sometimes, depending on where i put stored cube plates in the fridge, they turn blue if they were frozen for a short time.  usually near the back on top shelf, closest to freezer.  the cell walls burst from freeze/defrost, and bruise blue.
i haven't ever put plates like that to grains, i'd transfer once more to ensure viability, then go to grains.  but if u do try to go direct to grains, post results.




Too late, went to grains. :smile:

Of course I'll post results, I'm addicted to this stuff and this community too.

Well, it's very possible they got super cold -- but not that cold. I'm using a cloth tape and the agar is somewhere around 12 days old. Maybe the myc was going to pin, maybe not I don't know.

Either way, I transferred to new agar plates AND to grains so we'll have some more comparison.


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InvisiblemagicMerlin
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Registered: 01/26/12
Posts: 617
Loc: Toronto
Re: Blue veins on agar? [Re: blindingleaf]
    #20763577 - 10/28/14 11:59 AM (9 years, 4 months ago)

Quote:

blindingleaf said:
sometimes, depending on where i put stored cube plates in the fridge, they turn blue if they were frozen for a short time.  usually near the back on top shelf, closest to freezer.  the cell walls burst from freeze/defrost, and bruise blue.
i haven't ever put plates like that to grains, i'd transfer once more to ensure viability, then go to grains.  but if u do try to go direct to grains, post results.




Well, I just got the week off of work so I've been running the PC all the time. I was going to throw these old plates out since I have plenty of fresh growing ones, but with the extra grains laying around I think I'm going to try my first tiger drop. I'll keep a log and take pictures of anything interesting.


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OfflineSilvJailbiddi
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Registered: 06/10/21
Posts: 343
Last seen: 3 months, 6 days
Re: Blue veins on agar? [Re: magicMerlin]
    #27355880 - 06/20/21 12:17 AM (2 years, 9 months ago)

I got my agar culture delivered across 2000 miles by airplane , across various hot regions to my place which itself is really hot about 90F . That turned out be really dry with cracks and bluish discolouration of the agar underneath . This matches more or less the case with yours . I’m sharing pictures here of the before and after . 1 . While receiving and before packing 2. After arriving after 2 days by plane and road . 3 .Right After inoculation - 3rd day ( 2 jars ) . All the time I kept it inside fridge upside down inside a Saran wrapped insulated lunch box . I’m a first timer , I would appreciate any insight . Also I’m posting this mainly for people who asked pictures from the op. Thanks for this forum , I learnt a bit to get the confidence to start to cultivate .
Image 1 :


Image 2:


Image 3 :



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