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 agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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 Slow & inconsistent colonization 
#20507900 - 09/01/14 08:27 PM (9 years, 7 months ago) |
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Hi all- I'm just getting started with growing - using PF tek. I'm noticing that there is a huge inconsistency with how colonized my jars are. It's been 3 weeks since inoculation, and as you should see from the pics, some jars are 50+% colonized, and others have only one or two little spots of mycelium growth. From what I've read, it seems that most folks are at the 100% colonization mark at 3 weeks, and can birth at 4 weeks. So why are my jars going so slow? And why the huge inconsistency? At this rate, the slower jars won't be ready to birth for another 4 months!
I followed the PF tek closely, so I'm not sure what the problem is. All the jars have been located in the same location the whole time. I'm wondering if perhaps the syringe wasn't mixed enough. I got it from a sponsor site, but noticed that the spores seemed very clumped together in the syringe. It took a LOT of shaking to get them mixed up - like 5 minutes or so of shaking. But perhaps even that wasn't enough, and some spores ended up in a few jars, but a lot fewer in others? I did not pack the jars tight - tried to keep the mix light and fluffy.
Any ideas if I'm doing something wrong? Should the slower jars reach 100% eventually, or is there a point at which they should be birthed even if they're not at 100%? Thanks for helping a noob.
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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MonkeyJesusFresco
am i suspended in agar?
Registered: 10/09/12
Posts: 3,308
Loc: South East USA 
Last seen: 5 hours, 52 minutes
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 Re: Slow & inconsistent colonization [Re: agoraphone]
#20507909 - 09/01/14 08:28 PM (9 years, 7 months ago) |
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looks like bacterials in that second pic 
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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What part of it looks like bacterials? And you're not seeing that in the first pic?
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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 bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20508033 - 09/01/14 08:53 PM (9 years, 7 months ago) |
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first one is hard to tell take a more clear picture, natural lighting, white balance for the lighting, good focus.
second one is clearly fucked
third one is likely bacterial because it stalled and looks weak.
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supernewfie
some fella
Registered: 09/24/13
Posts: 1,103
Loc: Canada 
Last seen: 5 years, 7 months
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20508038 - 09/01/14 08:54 PM (9 years, 7 months ago) |
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How much water to verm did you add when preparing the cakes looks a little wet
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Re: Slow & inconsistent colonization [Re: bodhisatta]
#20508061 - 09/01/14 08:58 PM (9 years, 7 months ago) |
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Thanks for the opinions - can you spell out for a noob why the second one is fucked? Jar 1 & 2 look pretty similar to me. What are you seeing that says bacterials?
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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MonkeyJesusFresco
am i suspended in agar?
Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 hours, 52 minutes
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20508071 - 09/01/14 08:59 PM (9 years, 7 months ago) |
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and guys, make sure you're zoom'n in on that 2nd pic!
edit: that ropey-rhizo growth is a well known sight, in response to bacteria
Edited by MonkeyJesusFresco (09/01/14 09:01 PM)
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Re: Slow & inconsistent colonization [Re: supernewfie]
#20508085 - 09/01/14 09:02 PM (9 years, 7 months ago) |
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Quote:
supernewfie said:
How much water to verm did you add when preparing the cakes looks a little wet
2c BRF, 2c water, 4c verm
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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the left side of the jar looks different from the right side of the jar that's two different mycelium or your cube ran into bacteria it's thick looking it could also be another mold.
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Re: Slow & inconsistent colonization [Re: bodhisatta]
#20508146 - 09/01/14 09:15 PM (9 years, 7 months ago) |
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Quote:
bodhisatta said:
the left side of the jar looks different from the right side of the jar that's two different mycelium or your cube ran into bacteria it's thick looking it could also be another mold.
Ah- so it sounds like myc should look cottony, not stringy - yes?
When I see that stringy growth, does that mean that jar is not going to pin once it's birthed?
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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MoxyOx
Grazin'
Registered: 10/08/10
Posts: 1,439
Loc:
Last seen: 4 months, 5 days
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I don't see anything wrong with any of those jars aside from the water content. They are too moist, that is what is causing such a slow colonization.
If you see some form of bacteria, open paint and highlight it for the rest of us because I don't see it.
--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Quote:
MonkeyJesusFresco said:
that ropey-rhizo growth is a well known sight, in response to bacteria
I'm looking at this post from EvilMushroom666, and halfway down the page, in the colonization section, the 5th photo has the same stringy kind of growth. Are you saying that jar is contaminated too?
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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MudaFuka
Poppin bottles
Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Slow & inconsistent colonization [Re: MoxyOx]
#20508234 - 09/01/14 09:37 PM (9 years, 7 months ago) |
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1 and 2 are both bacterial. See how some of the mycelium looks like melted ice cream. that's bacteria in action.
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MonkeyJesusFresco
am i suspended in agar?
Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 5 hours, 52 minutes
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Re: Slow & inconsistent colonization [Re: MoxyOx]
#20509545 - 09/02/14 08:09 AM (9 years, 7 months ago) |
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Quote:
MoxyOx said:
I don't see anything wrong with any of those jars aside from the water content. They are too moist, that is what is causing such a slow colonization.
If you see some form of bacteria, open paint and highlight it for the rest of us because I don't see it.
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Re: Slow & inconsistent colonization [Re: MudaFuka]
#20510938 - 09/02/14 04:27 PM (9 years, 7 months ago) |
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Quote:
MudaFuka said:
1 and 2 are both bacterial. See how some of the mycelium looks like melted ice cream. that's bacteria in action.
OK so if it's stringy/ropy/looks like melted ice cream that means bacteria? I'm confused because I've seen a lot of photos with this kind of growth, with no indication that it's abnormal.
Again, does this pic (posted in EvilMushroom666's guide) also show bacteria?
Because to me, it looks similar to my jars. And if it's bacteria, then why would someone be posting it as an example in their guide? Or is there a difference between this jar and mine that I'm somehow missing?
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"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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MudaFuka
Poppin bottles
Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20511021 - 09/02/14 04:47 PM (9 years, 7 months ago) |
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Ropes are fine. No that jar of EM666 looks fine its not the ropes its the thick creamy white growth that signifies bacteria.
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Mike1230
Mycelium Motivator
Registered: 12/26/13
Posts: 555
Last seen: 3 years, 6 months
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20511115 - 09/02/14 05:04 PM (9 years, 7 months ago) |
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U see how his mycelium is covering the substrate, and how yours is leaving pieces of the sub exposed almost like water is stuck to the individual grains of sub? Well that's a good indicator of bacteria. Rhizo is ok but when it's malformed rhizo it's bacteria
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agoraphone
Stranger
Registered: 07/23/14
Posts: 43
Last seen: 3 years, 10 months
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Re: Slow & inconsistent colonization [Re: Mike1230]
#20511411 - 09/02/14 06:21 PM (9 years, 7 months ago) |
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Quote:
Mike1230 said:
U see how his mycelium is covering the substrate, and how yours is leaving pieces of the sub exposed almost like water is stuck to the individual grains of sub? Well that's a good indicator of bacteria. Rhizo is ok but when it's malformed rhizo it's bacteria
OK I think I'm starting to get it understand. Thanks for clarifying.
So if this were mold, I'd toss the jar, but it seems like it's growing, but just having a hard time due to a fight with some bacteria in the jar, yes? I'm thinking I'll keep seeing how the jars grow and try to fruit them anyway. This is only my second grow - first grow had some similar problems, but gave me about 12g dry harvest nonetheless.
I'm thinking about trying a different tek next time cuz apparently I suck at this one. I'm thinking about trying to buy pre-sterilized spawn bags and just inoculate those. Whaddayathink? Would that be a route that I'm less likely to screw up?
--------------------
"Always that same LSD story, you've all seen it. 'Young man on acid, thought he could fly, jumped out of a building. What a tragedy.' What a dick! Fuck him, he’s an idiot. [...] How about a positive LSD story? Wouldn't that be news-worthy, just the once? To base your decision on information rather than scare tactics and superstition and lies? I think it would be news-worthy. 'Today, a young man on acid realized that all matter is merely energy condensed to a slow vibration. That we are all one consciousness experiencing itself subjectively. There is no such thing as death, life is only a dream and we're the imagination of ourselves' . . . 'Here's Tom with the weather.' —Bill Hicks
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Slow & inconsistent colonization [Re: agoraphone]
#20511450 - 09/02/14 06:31 PM (9 years, 7 months ago) |
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Quote:
agoraphone said:
I'm thinking about trying a different tek next time cuz apparently I suck at this one. I'm thinking about trying to buy pre-sterilized spawn bags and just inoculate those. Whaddayathink? Would that be a route that I'm less likely to screw up?
trying to get this one to work again would be the best route in my opinion. You already have some experience with this, if you're going to give up on it then I have a feeling you'll do shitty elsewhere too. All you need to do is follow the directions to the T on this one and use quality sourced inoculant.
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MoxyOx
Grazin'
Registered: 10/08/10
Posts: 1,439
Loc:
Last seen: 4 months, 5 days
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Re: Slow & inconsistent colonization [Re: bodhisatta]
#20523238 - 09/05/14 08:18 AM (9 years, 7 months ago) |
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I agree, don't give up. Failure is part of life and should be dealt with accordingly by trying yet again.
--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.
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