|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
eskinz
Stranger
Registered: 04/01/14
Posts: 5
Last seen: 7 years, 8 months
|
Contaminated / dead syringes from sponsors?
#19981999 - 05/13/14 02:57 PM (9 years, 11 months ago) |
|
|
Friends,
I'd like to know how common having a bad product sent is. With three orders from three different vendors, I've yet to receive an order where 100% of the syringes were both clean and healthy.
Order A) 4 syringes. Two inactive, one contaminated, one ok. Order B) 3 syringes. One ok, two inactive. Order C) 3 syringes. One inactive/contaminated, two ok. (there's one more, but it's too early to tell).
My first suspect would be that I was introducing the problems on my end, however, I observe sterile procedure closely and the only variable are the syringes themselves. I haven't had a single failure with the syringes that appear to be ok, and I haven't had a single success with the syringes that may be bad. This is across multiple attempts with several growth mediums in all cases. In other words, these results seem to be very consistent.
Am I just really unlucky with the product that's been sent, or is this just the name of the game? I know that accidents can happen, even for experts (let alone, an amateur like me), but considering how consistent my results have been, I don't believe that I am introducing the issues I've been seeing.
I'm just getting kind of frustrated, and don't feel like I should have to provide lengthy explanations as to why the product is suspect, as opposed to my procedure.
Any thoughts on this?
Thanks, esKinz
(if this is better suited for the Sponsors subforum, please move it).
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: Contaminated / dead syringes from sponsors? [Re: eskinz]
#19982128 - 05/13/14 03:27 PM (9 years, 11 months ago) |
|
|
If they're vendor syringes its probably you that's the problem. I've never ran across or heard grom any other regular members of bad syringes from vendors
|
Spa
PoohHandler
Registered: 04/04/14
Posts: 153
Last seen: 9 years, 10 months
|
Re: Contaminated / dead syringes from sponsors? [Re: bodhisatta]
#19982214 - 05/13/14 03:45 PM (9 years, 11 months ago) |
|
|
I've gotten a VERY slow colonizing syringe off an ex vendor, which I know wasn't the procedures fault cause I nocd jars from the same batch of jars with Cambodian and have already spawned to sub which is at about 70% now and did some g2g that is at 90%. All was originally started a month and maybe a week ago. That Cambodian is a beast!! The other strain is only at maybe 30% in the qt jars:-/
So IMO it's very possible to get a shity syringe. But I highly doubt all of you're problems are the syringes fault. What teks and what sterilize procedures are you using?
-------------------- If you are lucky enough to find a way of life you love, you must find the courage to live it
|
Spa
PoohHandler
Registered: 04/04/14
Posts: 153
Last seen: 9 years, 10 months
|
Re: Contaminated / dead syringes from sponsors? [Re: Spa]
#19982231 - 05/13/14 03:48 PM (9 years, 11 months ago) |
|
|
Just checked my first post on here. Actually the 17th marks one months exactly! Damn that Cambodian strain in insane!! Sorry to be so irrelevant to the OP, but wow....
-------------------- If you are lucky enough to find a way of life you love, you must find the courage to live it
|
lateforthafuture
fanatic
Registered: 03/26/07
Posts: 471
Last seen: 3 years, 6 months
|
Re: Contaminated / dead syringes from sponsors? [Re: eskinz]
#19982568 - 05/13/14 04:55 PM (9 years, 11 months ago) |
|
|
Trust no spore solution, put it to agar then go from there. The sponsors do they're best and most times syringes are clean, but I've gotten some dirty syringes from long time sponsors recently. Spore prints and spore solution are never 100% contam free. Not saying that you wont get clean syringes often, but you'll never get clean syringes 100% of the time from even the best sponsor vendor. To progress you must learn agar so you can see what your working with and transfer away from contams.
-------------------- "Not what could have been, or what should have been, but what will be" -Self
|
36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,086
Loc: Diving into Mystical Territori...
|
Re: Contaminated / dead syringes from sponsors? [Re: lateforthafuture]
#19982894 - 05/13/14 06:06 PM (9 years, 11 months ago) |
|
|
Slow growth is from using MS solution. Nothing to be done about it except to isolate better genetics.
As for you failing all those times, OP, it's gotta be you. I could see if you had one or maybe 2 failures, but after that many times you need to review your sterile technique. You say it's good, but maybe it's not as good as you think.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
DetectiveLefty
Nullius in verba
Registered: 10/12/13
Posts: 177
Loc: Floriduh, USA
Last seen: 4 years, 1 month
|
Re: Contaminated / dead syringes from sponsors? [Re: 36fuckin5]
#19982949 - 05/13/14 06:16 PM (9 years, 11 months ago) |
|
|
With multispore, about 25-50% of my jars contaminate at some point. I've had the best luck inoculating several fahtjars (about 3 tbsp hydrated popcorn in a 4-oz. jat with tyvek lid and silicone inoculation port) with *one* drop of spore solution, then making myc water syringes from one of the uncontaminated jars. I was able to get a clean culture from a spore syringe that contaminated in 4/4 BRF jars with this method.
-------------------- I swear on a stack of Bibles that everything I said in the above post is the 100% literal Truth.
|
eskinz
Stranger
Registered: 04/01/14
Posts: 5
Last seen: 7 years, 8 months
|
Re: Contaminated / dead syringes from sponsors? [Re: DetectiveLefty]
#19986172 - 05/14/14 09:20 AM (9 years, 11 months ago) |
|
|
Just for clarification on the mediums, I have been using agar for some time now (and have learned it's necessary due to what I believe are quality control issues). And even there, known good ms syringes will be fine and the questionable ones appear either dead or contaminated. The same is for ms to liquid culture.
I guess I can dig back into this PF decontamination tek and keep my fingers crossed.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: Contaminated / dead syringes from sponsors? [Re: eskinz]
#19986180 - 05/14/14 09:24 AM (9 years, 11 months ago) |
|
|
Spore syringes are never 100% clean but they don't need to be for good results. Vendor syringes are the cleanest you'll get. Likely in-vitro fruits and use of a flow hood and experience. Home made syringes I would worry about but even still I can make a fine syringe and as I said vendor syringes have never failed me once in over 10 syringes
|
Cryok
Stranger
Registered: 05/11/14
Posts: 6
Last seen: 9 years, 6 months
|
Re: Contaminated / dead syringes from sponsors? [Re: bodhisatta]
#19986246 - 05/14/14 09:44 AM (9 years, 11 months ago) |
|
|
Interestingly enough, I bought some syringes from a non-shroomery sponsor (didn't do much research:( ) and they were absolutely horrible. Little to no growth at all, even after 3 weeks.
I'm getting some proper prints from a sponsor soon and I really hope they'll be much better....
-------------------- Life is a gift, use it wisely.
|
blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Contaminated / dead syringes from sponsors? [Re: Cryok]
#19986259 - 05/14/14 09:49 AM (9 years, 11 months ago) |
|
|
the only syringes i bought from non vendors turned out to be an LC hidden within a charcoal water. i still put them to agar before getting culture on grain.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
|