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PsleepyHead
Strangest
Registered: 02/11/13
Posts: 9
Last seen: 8 years, 7 months
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Does the dormancy period of spores effect gene expression? 1
#19739065 - 03/23/14 11:19 PM (10 years, 27 days ago) |
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So this MS syringe was sitting around for a very long time before it inoculated some half pint cakes. Like 9 months at least and the syringe was subject to highly variable conditions, though I don't think it ever reached 90*F or higher. I'm not an active poster here but I've been lurking for a long time. This is the longest that I've known of spores being stored and producing mycelium. I think a year ago I read a thread that someone had stored spores for 4-5 months or so and used them successfully but I don't remember any specifics of how the fruits turned out or how fast they grew.
Does anyone here have a grow log where the spores were dormant for many months (years?) prior to inoculation, especially spores that weren't stored in a fridge? Did you observe anything different about that particular grow?
These jars took a little longer to start developing mycelium than what I consider normal, I think 7 or 8 days. I'm thinking maybe the extended dormancy period allowed only slower-metabolising spores to survive, or maybe gene expression is affected by the dormancy period through some kind of trigger, or maybe novice mycologists just get a little carried away with their speculation.
Other important things to note about these cakes:
These BRF cakes were steam sterilized in a stockpot with a strainer that fit into it, like what you'd cook spaghetti noodles in. The strainer kept the jars suspended over a good amount of water. The lid had a 1/8" hole that can be plugged with a small piece of plastic, and a gasket was made for the lid edges by double folding a piece of duct tape. The temperature on the burner was regularly adjusted so that condensation stayed on the jars and the water remained boiling. The water wasn't quite at a rolling boil but was certainly bubbling. Two batches of 5 jars were steamed this way. For each batch, a timer was set to 150 minutes. Every time the strainer was lifted out to add hot water, I would add 15 minutes of time to the timer. Water only needed refilling about once an hour. Each batch took an estimated 3 hours to sterilize.
I know this sterilization method isn't an official tek anywhere and I know not following teks to the T is frowned upon here, but I think maintaining the condensation on the jars and just steaming them for a really frickin long time does the trick and compensates for the lack of pressure. I may be wrong, though. I'll let you guys know what happens.
Poor sterile procedure during inoculation! One jar is infected with a dark green spot right at the inoculation site, immediately under the verm layer. Other than that no other infections are visible BUT many of the inoculation sites lack any mycelium at day 12. This makes me think maybe another organism has a foothold on the substrate and isn't allowing the mycelium to develop. About what fraction of infections are visible, half, more, or less? The syringe was shaken pretty well before inoculation.
Sorry for the lengthy post. Thanks for any help!
-------------------- The person born with a talent they are meant to use will find their greatest happiness in using it. -Johann Wolfgang von Goethe
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Pinkfloyd777
The infidel
Registered: 02/11/14
Posts: 490
Last seen: 6 years, 5 months
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Re: Does the dormancy period of spores effect gene expression? [Re: PsleepyHead]
#19739135 - 03/23/14 11:40 PM (10 years, 27 days ago) |
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I would suggest moving this to the advanced mycology thread.. I have not been around in the mycology world to help you with this and I don't think any of us on the cultivation thread have let a syringe sit for 9 months before.. Just my 2 cents..
-------------------- ~Mexican cub~ (first grow) frequent updates http://www.shroomery.org/forums/showflat.php/Number/19721736 life is a beach.. I'm just playing in the sand
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Does the dormancy period of spores effect gene expression? [Re: Pinkfloyd777]
#19739348 - 03/24/14 12:56 AM (10 years, 27 days ago) |
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no need to sterilize them that long, 90 minutes is all thats needed and it's not uncommon for syringes to last longer then a year
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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