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OfflineAlbertHoffmann
Professor

Registered: 09/15/03
Posts: 29
Last seen: 15 years, 25 days
No Growth, what should he do
    #1917862 - 09/15/03 10:45 AM (20 years, 6 months ago)

About a month and a half ago this guy received some spores from Sporeworks. He was about to go on vacation, so he put them in the fridge. 4 weeks later he prepared his jars. 7 pf's, and 10 birdseed(w/ a little whole brown rice mixed in) The PF's were pressure cooked for 1 hour, and the seed for two hours. 1/2 of each set of jars was prepared w/ tyvek for gas exchange. Two days later, the jars were injected. He used 3 different strains. (not in each jar, but a couple jars of each). Now it has been 12 days, and there is not a single sign of growth in ANY of the jars. The temp has been between about 73-85.
What should he do? Could Sporeworks, or maybe shipping be to blame? It was still pretty hot around here a month and a half ago, when the package arrived. Could the month in the fridge be the problem?
Any ideas would be greatly appreciated.


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Offlinejba6511
addict
Registered: 11/04/02
Posts: 495
Last seen: 16 years, 3 months
Re: No Growth, what should he do [Re: AlbertHoffmann]
    #1917874 - 09/15/03 10:50 AM (20 years, 6 months ago)

did u allow enough time for the jars to completely cool before injecting?

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Anonymous

Re: No Growth, what should he do [Re: AlbertHoffmann]
    #1917875 - 09/15/03 10:50 AM (20 years, 6 months ago)

only been 12 days...u need to wait a little longer before getting worried....

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Anonymous

Re: No Growth, what should he do [Re: jba6511]
    #1917878 - 09/15/03 10:51 AM (20 years, 6 months ago)

Quote:

jba6511 said:
did u allow enough time for the jars to completely cool before injecting?




"The PF's were pressure cooked for 1 hour, and the seed for two hours. 1/2 of each set of jars was prepared w/ tyvek for gas exchange. Two days later, the jars were injected"

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OfflineSam_Kohui
if you onlyknew...

Registered: 08/23/03
Posts: 140
Last seen: 16 years, 5 months
Re: No Growth, what should he do [Re: ]
    #1917930 - 09/15/03 11:25 AM (20 years, 6 months ago)

did you shake the syringe before injecting?


--------------------
The PF Tek...it's a piece of cake (the BRF kind :cool: )

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OfflineAlbertHoffmann
Professor

Registered: 09/15/03
Posts: 29
Last seen: 15 years, 25 days
Re: No Growth, what should he do [Re: Sam_Kohui]
    #1917971 - 09/15/03 11:46 AM (20 years, 6 months ago)

Wait longer you say.. All these posts about people w/ fully colonized jars after a week, and growth after a day have got him in a rush, I guess.
Syringes were shook.

Thanks for the quick replies!

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Anonymous

Re: No Growth, what should he do [Re: AlbertHoffmann]
    #1918240 - 09/15/03 01:22 PM (20 years, 6 months ago)

it took my recent jars over a month and a half to fully colonize

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Invisibleund3rw0rld

Registered: 09/02/03
Posts: 50
Re: No Growth, what should he do [Re: ]
    #1918253 - 09/15/03 01:29 PM (20 years, 6 months ago)

after 12 days with no signs of growth, if i were you id start over..if you grow anything now it will probably be contams..dont waste time to find out its not goin to colonize..just start over

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Anonymous

Re: No Growth, what should he do [Re: und3rw0rld]
    #1918265 - 09/15/03 01:33 PM (20 years, 6 months ago)

dont liten to that man....keep checking them........it took my damn bitches forever to colonize

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Invisibleund3rw0rld

Registered: 09/02/03
Posts: 50
Re: No Growth, what should he do [Re: und3rw0rld]
    #1918266 - 09/15/03 01:33 PM (20 years, 6 months ago)

my buddy finds birdseed to be a royal pain, messy, and hasnt worked for him, yet many people have great success with it..try rye berries its so much easier and you can find them in health food stores if you cant find a place to buy em just do a google search and buy em online

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Invisibleund3rw0rld

Registered: 09/02/03
Posts: 50
Re: No Growth, what should he do [Re: und3rw0rld]
    #1918280 - 09/15/03 01:36 PM (20 years, 6 months ago)

why did you wait 2 days before injecting the jars ? you should do it when the jars are cool but 2 days is pushing it...my buddy finds that the longer he waits the greater the chance for contam

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Anonymous

Re: No Growth, what should he do [Re: und3rw0rld]
    #1918303 - 09/15/03 01:42 PM (20 years, 6 months ago)

i doing my 1st grow with birdseed...it pissing me off...it took almost two months to fully colonize the jars...and now the casing been around over a month and its just now finally pinning...and its not even fully covered.....fucking wbs

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Invisibleund3rw0rld

Registered: 09/02/03
Posts: 50
Re: No Growth, what should he do [Re: ]
    #1918709 - 09/15/03 04:06 PM (20 years, 6 months ago)

wow sorry to hear that..my buddys experience with rye berries produces fruits within a month from syringe and 3 weeks with a grain to grain transfer...so for the 3 months you waited for that WBS you could have had 3 goes at it with rye

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