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Will update OP -

7 of 8 PP5 Containers show noticable growth - inoculated in glovebox using syringe on 10/25 around 5pm

The LC jars inoculated on 10/24, hard to tell, I think I did Tablespoons of Karo instead of TSP so the liquid is very dark, a flashlight does not help either. We will see in a week or so whether or not they will be viable.

inoculated ten quarts ball jars on 10/26 early midnight and 10/26 mid-day

We will see how they look here in a few days.

The pp5 RGS is 2 - 1/2 pints of each variety I have ordered, and one will be used for g2g, and another will be fruited for spore prints to go onto agar.

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10/30 Update
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11/3
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Some pictures - will add information later.


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11/8
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Here is a photo update! If you look from the last photos to these, you ccan see I've added a few more jars



The two pictures of the single jar, I am concerned about -

It looks really heavy, albeit it may be caused by the grain shrinkage and it trying to reach out to the glass - You be the judge of that - I was going to do another ten or so jars tomorrow using that a master g2g -

Its hasnt been that long, but it looks like my original idea of having a lasting supply from a few cc's is on its way to becoming a reality - thanks to all who have helped and inspired me along the way!

Onward!

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11/18
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I've done my first 56 Qt Mono -

I've got another 3 16 qts mini-monos and 4 6qt Mini-Mini-Mono's
Going aswell. Update on Jars I have 36-40 Quarts of Spawn Going
Currently, 10 or so plates of Agar - Including some Wild Aussie
spores generously sent from another member!

Onward!

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“The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.”
― Alan Wilson Watts, The Culture of Counter-Culture: Edited Transcripts

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Stromrider said:
Another poor victim

I have a start to finish bulk tek in my journal. These methods I am advocating in my journal are tried and true. Check it out if you really wanna grow some mushrooms

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Stromrider said:
Another poor victim

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Stromrider said:
I was referring to you wanting to grow with the Violet tek but I really don't wanna start up this debate again. I just hate to see people put all that time and effort in just to end up with a bunch of poor spindly looking shrooms.

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Look new fella, if you "really don't wanna start up this debate again" then why would you drop those kinds of false statements?
I see you dropping untrue and half-true assumptions all-over these forums lately, often following or stirring such childish dramas.


In my experience it's pretty safe to say that if people don't get good results they either don't know proper conditions or the water element, or they don't select genetics; that or they simply did poorly somehow else, a newbie is a newbie.

Mushrooms don't grow "spindly" because of a tek. Try convincing anyone that straight-grain necessarily results in "spindly" mushrooms.
If you really believe it does you are simply ignorant to the obvious facts.

Some first flushes... (multi-spore even)


3rd flushes from some mediocre clones...


5th flushes even!

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Stromrider said:
Your time and effort would be much better spent using time tested methods that yeild undeniably great results.

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Of course using bulk substrates is an entirely viable way to grow, especially to people who don't yet fully understand providing proper moisture and humidity conditions to their grows, and for those that prefer lean on the roundabouts of a tek instead of an implemented in-depth understanding of the multiple grow facets.
With both, even, results can be incredibly impressive and one could only be exited about pulling off results like this.
I can't help but wonder how growers like him, who truly get conditions for their grow method, could do if they gave a shot to adjusting their knowledge to a method like mine. Certainly he would do even better than me.

I had quite bumper output with bulk many times myself:

But as I had them and watered cased grains next to each other in the same chamber I began to see their differences. I definitely had to tweak my conditions and process, but once I learned the simple subtleties of ideal conditions (which bulk did not really help me learn but instead works around) I realized the watered cased grains were giving me a better yield over time and cut many big steps out of my process.  Not to mention they dried more quickly and people liked them more...  I can't imagine an experienced grower not catching that happen right in front of him of her.  Big parts of my process went entirely unused; the biggest sub, the biggest water, the biggest energy, the biggest clean work, the biggest failure risk juncture, the biggest garbage. Yet my big output stayed consistent and my friends start telling me they can plainly tell a difference too.
As they say, ""

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Violet said:
Thanks!  They're the same exact culture in fact

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http://www.fpl.fs.fed.us/documnts/pdf1994/burds94a.pdf

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This is the reference article -

Preserving Cultures Using Sterile Distiled Water in Cryovials

Abstract:

Prior to 1985, cultures at teh center for forest mycology research were maintained on a 1.5% malt extract agar test-tube slants. This system not only made it neccessary to transfer the entire collection every year but also permitted genetic change because continual growth occured. In 1985, the method of storing fungal cultures in sterile distilled water in CRYOVIALS was introduced. This study reports on the use of this method for long-term fungal storage. For varying periods of up to seven ( 7 ) years, 151 miscellaneous specials of wood-decaying Basidimycotina were stored in sterile distilled water. Water storage has numerous advantages: culture viability or growth rate is not significantly influenced; isolates can be stored longer; genetic stability is greater; the method is quick, easy, and inexpensive, and requires less space.

Cyrovials ( Internally Threaded & Self Standing

These can withstand autoclaving so if I were to buy them and try something along the lines of distilled water long term storage, I could always uses them as slighty more expensive alternative to the glass vacutainer blood collection tubes.

Heres what Im guessing - seven years, of unrefigerated storage is a possiblity, but even two years unrefigerated would be nice aswell.

Full Sterile - with option of threaded inside, or external -
which would you prefer - internal or external threads?

Take a fully colonized grain, or spore print - isolate, isolate, gather your monoculture - inside of SAB/GB/FLOW/FKN LABRATORY

Take 4-6-8 ML of Sterile Distilled Water and put onto your agar monoculture

Scrape it a bit, mix up the myc.

Suck it back up and put into the Cyrovial -

Than go about your business and save for 5 years and when needed re-enter onto agar - break down any contams if any - and sustain your strain? Or take fully colonized agar chunk, cut into 2x2 pieces and drop three or four into the cyrovial filled with sterile distilled water, seal and store. They feature a rubber type gasket internally and can be twisted completley air-tight aswell, so no need to wrap them.

Im new to all of this, so this all might be a huge waste of brainpower. Please leave any and all feedback, discreation unneccesary, so... what you think? 

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JMcDoogle said:
Thanks for the replys. Your input will not go unnoticed. Heres what I think i'll be doing once everything arrives.

Today, I'll be making my way to the store to purchase 50# or so of Rye Grass Seed (from now on i'll be using RGS to shorten it up.) My spores will be arriving today, though my new 23qt pressure cooker probably not until tuesday. I may begin tomorrow to saturate the grains and allow them time to dry properly, perhaps even indulge in the plant food avenue... perhaps. So I'll have my syringes, but my agar petri dishes will not be here until tuesday most likely. I have hundreds of 10cc individual wrapped syringes, i'll ask a question of those later. So without the Agar prepped and ready as of now I believe I will be using the spore syringes as early as tuesday night / wednesday morning once the pc has arrived and I can properly sterilize the grains.

I believe strain questions should be directed to a single thread, but as this is my topic I believe I am okay in asking which one do you believe I should start off with.

I have yet to create a GH - though I do have 2 extra walk-in closets with little to no airflow measuring at 8x4 - I believe this space will be sufficient.

I find it hard to believe sterilite and other clear containers can range up to 30-40-50$ a piece, wow, looking forward to finding some at a discount somewhere.

So, to sum up the questions...

If possible, could anyone recommend the most cost-effective GloveBox - Zero Air Box construction.

What are the expected limitations in such a room 8x4 with 10 foot ceilings. ( I do have an extra room 13x12 with closet, though very dusty and has furnace inlet/outlet. )

Should I wait for all of the agar materials to arrive, thus creating glovebox to create agar petri dishes using Spore Syringes from LSS, or perhaps create a spawn jar by using 1-4cc of syringe in a traditional PF-TEK style jar using RGS or BRW/VERM - than use living culture from the jar for g2g transfers, hope everything goes right and create spore prints / pin or flesh agar transfers, perhaps maybe liquid culture?

I will be trying the use of Ziplok twist-n-seal and using Violets tek for my first attempt at my newfound hobby. Anything else I should think of -

Again thanks for reading my grumblings if you have, I know I am not the most informed/organized, but if have waded through this post and came out unharmed, please do offer any thoughts/inputs you may have without discretion.

EDIT: Oh, I forgot to ask, with the individually wrapped sterile 10cc syringes, I have four long blunt applicator needles that will fit perfectly, if I were to make a LC of some sort, would I be able to take of the applicator needle, and leave uncapped of any sort, and perhaps wrap with foil, I do not have any covers for them of any sort - just the four long steel applicator needles. Thanks!

“Things are as they are. Looking out into the universe at night, we make no comparisons between right and wrong stars, nor between well and badly arranged constellations.”

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