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InvisibleStropharis
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Stro's Agar Prep * 8
    #18431006 - 06/17/13 07:18 AM (10 years, 7 months ago)

Stro's Agar Prep


I will be using Malt Extract Agar for this write up but preparation remains the same using other formulas.

Super simple.

Things you will need

Scale


Distilled water


Autoclave flask or in my case a whiskey bottle.


Lid and Small peace of tyvek


Two small peaces of tinfoil


Agar-Agar


Malt Extract


Hefe/Yeast (optional)


And all the other obvious items such as a pressure cooker, still air box or flow hood, dishes.


The recipe; enough for 20 dishes (one sleeve)

500ml distilled water (1/2 liter)
10g Agar-Agar
10g Malt Extract
1g yeast



Mix the powders with the appropriate amount of water inside your bottle and give it a few wrist twirls to mix it together. You don't have to do the full amount, in fact I usually do smaller batches, for example; if you split it in half and use 5 grams of agar, 5 grams of malt extract, and .5 grams of optional yeast with for 250ml of mix; this will equal about 10 dishes.

After you put your water into your bottle and mix it with your powders, place the piece of tyvek on top of the bottle opening.




Next place your cap on top and tighten it down.



I put my first peace of foil over it and squeeze it down tight, the next I just add for extra protection.



It's ready to load into your pressure cooker. In addition to the metal bottom rack plate I add a small towel although it isn't necessary.



Once the bottle is loaded into your pressure cooker and ready for sterilization, fill it up to where the water level is no more than one or two inches below your agar mix, the closer the better I have found, this will help prevent boil over when its cooling down. I have a small whiskey bottle and small pressure cooker so it sits in leaning sideways against the side of the pressure cooker so I can actually have the water level near level with the agar mix inside the bottle.

Pressure cooked for 30 min @ 15psi, using a pressure cooker with a rocker pressure regulator, a slight rocking motion will signify roughly 15psi. 

Once done let your pressure cooker depressurize and cool on its own. Any time after your bottle is cool to the touch it can be poured but don't wait too long or it will begin to solidify. Make sure your area is cleaned and wiped down with a disinfected and the air is sprayed with air sanitizer. In addition make sure you get cleaned up, take a shower, wash your hands with anti bacterial soap, wear gloves and wipe down your bottle and hands with isopropyl alcohol before moving it into the still air box and getting to work.

Here is a few photos taken for the purpose of the write up to show how to pour dishes, I am outside the still airbox and I am not actually pouring anything for the purpose of this write up.

Working in a still air box space can be limited so I usually work in stacks of five dishes, sometimes ten depending on how drunk I am. You will want to start on the bottom of the stack and work your way up, you lift the entire stack of dishes with the lid of the first dish to be poured.



and moving up to the next dish, again lifting the rest of the stack with the lid of the dish to be poured.





And so on




Once done, stack all your dishes and angle yourself around to cover them with either the sleeve they came in or use smaller stacks and cover with ziplock bags. I let them cool with the bag siting over them inside the box for an hour or two, once done I get gloved back up to close the bag or start working if I have agar work that needs to be done.

Hope this helps! See Stro's Cleaning and Isolating On Agar next.


- Stro


Edited by Stropharis (04/14/15 03:03 PM)


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OfflineMr.Cue
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Re: Stro's Agar Prep [Re: Stropharis]
    #18432006 - 06/17/13 01:24 PM (10 years, 7 months ago)

Can I use light malt extract?


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Mr.Cue]
    #18432243 - 06/17/13 02:31 PM (10 years, 7 months ago)

Yes it should works fine, at least from what I have read. I have never worked with it, you can see listed on the shroomery's main info pages, they have the MEA mix using Light Malt Extract (LME) opposed to Dried Malt Extract (DME). I am assuming your LME is in liquid form right? I think it's 20% water, like a syrup? On the other hand DME is a powder.

Home | Mushroom Info | Growing Mushrooms | General Cultivation | Agar and Culture Storage | What are some good agar media recipes?


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OfflineMr.Cue
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Re: Stro's Agar Prep [Re: Stropharis]
    #18432274 - 06/17/13 02:40 PM (10 years, 7 months ago)

No this is a powder.
http://www.sears.com/shc/s/p_10153_12605_SPM7769608311P?ci_src=184425893&ci_sku=SPM7769608311&sid=IDx20130125xMPFDGx011

I need to get some myco supplies tomorrow any way, so I'll try to stop buy a home brewing store and see if they have any malt extract. I'll just save this for LCs.


Edited by Mr.Cue (06/17/13 02:46 PM)


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Mr.Cue]
    #18432284 - 06/17/13 02:43 PM (10 years, 7 months ago)

How do you figure that is Light Malt Extract?

It says Dried Malt Extract, not only that but it actually says Dark Dried Malt Extract, lol.


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OfflineMr.Cue
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Re: Stro's Agar Prep [Re: Stropharis]
    #18432295 - 06/17/13 02:47 PM (10 years, 7 months ago)

Yeah sorry, I just caught that and changed the link.


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Stropharis]
    #18432306 - 06/17/13 02:49 PM (10 years, 7 months ago)

Light malt extract or dark, it will work none the less. I only have experience with malt extract in the powder form though, I personally refer to all of those as DME.

I am willing to bet both of those work fine.


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OfflineMr.Cue
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Re: Stro's Agar Prep [Re: Stropharis] * 1
    #18432371 - 06/17/13 03:01 PM (10 years, 7 months ago)

I just found the conversion rate on a home brewing site.
"The conversion rate is 1 DME = 1.25 LME"

So I'll just use 12.5g of LME to equal your 10g of DME.
And, I'll stop hijacking your thread.
Thanks


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Mr.Cue] * 1
    #18432378 - 06/17/13 03:03 PM (10 years, 7 months ago)

Nah Mang, thats what its for, to help out.

I actually didn't know that, good find.


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OfflineMr.Cue
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Re: Stro's Agar Prep [Re: Mr.Cue]
    #18432409 - 06/17/13 03:12 PM (10 years, 7 months ago)



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Offlinebburnett227
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Re: Stro's Agar Prep [Re: Mr.Cue] * 1
    #21920216 - 07/09/15 02:28 PM (8 years, 6 months ago)

Sorry if it's "unacceptable" to revive thIs Thread but it seems like a good one.

Just curious though as to what the upside is to the yeast and if it makes that big of a difference in growth.

To use or not to use.

Thanks!


--------------------
Abe


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OfflineGreenRabbit
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Re: Stro's Agar Prep [Re: bburnett227]
    #21920365 - 07/09/15 03:07 PM (8 years, 6 months ago)

Haven't seen anyone using yeast in agar recently.
Pastywhytes tek leaves it out.

Not sure what the yeast is for; you won't have a problem leaving it out.


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OfflineBLINKfan420
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #21920465 - 07/09/15 03:26 PM (8 years, 6 months ago)

I've been looking for a thread like this for weeks!! Why did this not come up in my 100s of searches?!#*#\!

*****EDIT: re-read thread and looked at pictures. Found my answer.  Drill a hole in the lid of the bottle for pressure change.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


Edited by BLINKfan420 (07/09/15 03:28 PM)


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Offlinebburnett227
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Re: Stro's Agar Prep [Re: BLINKfan420]
    #21920481 - 07/09/15 03:28 PM (8 years, 6 months ago)

If u look at the picture of the lid there is a hole in it. If u didn't have a hole u wouldn't need the tyvek


--------------------
Abe


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Offlinebburnett227
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Re: Stro's Agar Prep [Re: bburnett227]
    #21920487 - 07/09/15 03:28 PM (8 years, 6 months ago)

U edited just as I posted lol


--------------------
Abe


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: bburnett227]
    #21921614 - 07/09/15 07:58 PM (8 years, 6 months ago)

Yeast is a nutritional supplement, adding it to agar formulas provides essential vitamins, amino acids, and nitrogen. This enhanced the speed and quality of mycelium growth and it's also been said that it stimulates spore germination.


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Offlinebburnett227
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Re: Stro's Agar Prep [Re: Stropharis]
    #21921701 - 07/09/15 08:17 PM (8 years, 6 months ago)

Thanks for the info, so its really not a make it brake ingredient.  Good.  Thanks for the tek by the way. Using it right now, actually, waiting on my bottle to cool some.


--------------------
Abe


Edited by bburnett227 (07/09/15 08:19 PM)


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Invisiblemaddchef
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Registered: 09/04/09
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Re: Stro's Agar Prep [Re: bburnett227]
    #21922837 - 07/10/15 12:51 AM (8 years, 6 months ago)

Stro, I've always seen faster rhizomorphic growth from low nutrient plates. While it surely would be more complex and nutritious have you ever done a side by side with yeast and or peptone?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: maddchef]
    #21925573 - 07/10/15 05:57 PM (8 years, 6 months ago)

I haven't done my own comparisons no, sounds interesting though. I do know that sometimes reaching hyphea on the hunt for more food can appear faster but it isn't the best quality or the thickest healthy mat.


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Invisiblemaddchef
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Re: Stro's Agar Prep [Re: Stropharis]
    #21925985 - 07/10/15 08:18 PM (8 years, 6 months ago)

You're right about the "quality" and thickness. I have had hyphae on low nutrient plates gravitate towards each other and form a mass to the point my plates looked almost like snowflakes or stars.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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