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Offline760Rollz
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inoculation loop sterilization
    #15040430 - 09/06/11 10:39 PM (12 years, 6 months ago)

I am kind of worried that I could kill spores if I flame sterilize my inoculation loop and then use it to quickly before it has cooled down.  If I sterilize my inoculation loop with an alcohol lamp outside of my glove box and then wipe my loop on an alcohol pad once it gets inside the glove box would it work to cool the loop enough?  Or would this just be overkill? ...Gawd so many questions as I'm just about to get started:crazy:


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OfflineSexySmurff
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Re: inoculation loop sterilization [Re: 760Rollz]
    #15040452 - 09/06/11 10:45 PM (12 years, 6 months ago)

you're actually going backwards by doing that.  alcohol doesn't sterilize it sanitizes.


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Offline760Rollz
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Re: inoculation loop sterilization [Re: SexySmurff]
    #15040552 - 09/06/11 11:16 PM (12 years, 6 months ago)

So then it wouldn't be over kill?  So it would cool the loop down enough to use?  Or can I just move from flame, to box, to print and make a syringe without worrying about destroying living spores because the loop has not had enough time to cool down?


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Invisiblehoobatech

Registered: 08/16/04
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Re: inoculation loop sterilization [Re: 760Rollz]
    #15040565 - 09/06/11 11:19 PM (12 years, 6 months ago)

the wire is really thin, just hold it for 20 sec or however long it takes to cool to the touch

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InvisibleMeetzu
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Re: inoculation loop sterilization [Re: 760Rollz]
    #15040568 - 09/06/11 11:21 PM (12 years, 6 months ago)

Quote:

760Rollz said:
So then it wouldn't be over kill?  So it would cool the loop down enough to use?  Or can I just move from flame, to box, to print and make a syringe without worrying about destroying living spores because the loop has not had enough time to cool down?



He is saying it's not something you should do because alcohol does not kill mold spores but heat does.


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Invisiblesandman420
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Re: inoculation loop sterilization [Re: Meetzu]
    #15040736 - 09/07/11 12:32 AM (12 years, 6 months ago)

heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.


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OfflineRogerRabbitM
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Re: inoculation loop sterilization [Re: sandman420]
    #15041493 - 09/07/11 07:44 AM (12 years, 6 months ago)

Quote:

sandman420 said:
heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.




This is the correct procedure.  Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower.
RR


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InvisibleDoctor_Inoc
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Re: inoculation loop sterilization [Re: RogerRabbit]
    #15041624 - 09/07/11 08:42 AM (12 years, 6 months ago)

Quote:

RogerRabbit said:
Quote:

sandman420 said:
heat the loop red hot, then cool it by sticking it into the agar of the receiving dish for a few seconds.




This is the correct procedure.  Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower.
RR



Damn dude!  :rofl2:  :lol:

:whathesaid:

:peace:

DI

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Offlinesteelmonkey
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Re: inoculation loop sterilization [Re: Doctor_Inoc]
    #15041782 - 09/07/11 09:22 AM (12 years, 6 months ago)

Very graphic,but it made me laugh all the same


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OfflineWing
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Re: inoculation loop sterilization [Re: steelmonkey]
    #15042259 - 09/07/11 11:21 AM (12 years, 6 months ago)

RR cracks me up!


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InvisibleLayinUp
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Re: inoculation loop sterilization [Re: RogerRabbit]
    #15042320 - 09/07/11 11:31 AM (12 years, 6 months ago)

Quote:

RogerRabbit said:
...wiping shit on your face after taking a shower.
RR




:feelsbadman:


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Offline760Rollz
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Re: inoculation loop sterilization [Re: LayinUp]
    #15054382 - 09/09/11 05:22 PM (12 years, 6 months ago)

lol Thanks all.


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