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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Mold or something else?
#14343021 - 04/24/11 10:59 AM (12 years, 10 months ago) |
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The first couple pics are of this jar where the substrate has taken on a grey color. I had one with green and threw it out.


Now I wanna know if I should leave it, birth it or PC it and throw it out?
annnd the second pics are just to check on my progress. You guys think I should birth them? They were inoculated March 15th.

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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Mold or something else? [Re: thatsnice]
#14343027 - 04/24/11 11:01 AM (12 years, 10 months ago) |
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I'd toss that first one. No special hurry, you could isolate it and observe for developments.
-------------------- You make it all possible. Doesn't it feel good?
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? [Re: Doc_T]
#14343056 - 04/24/11 11:09 AM (12 years, 10 months ago) |
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Why should I observe it, would it be safe to eat off of? and thanks for the response.
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mister
Nature nut



Registered: 10/04/08
Posts: 2,926
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Re: Mold or something else? [Re: thatsnice]
#14343213 - 04/24/11 11:55 AM (12 years, 10 months ago) |
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It may just have run into an area of lower moisture. unless there is an area of green going on. Set it to the side and watch it. The sub may have also compacted and that could of made it harder for the mycelium to break through that area. throw out when it goes green for sure. Just watch and wait, but do not open in your house. Go outside, or pc them then discard to kill the mold, but this will stink.
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   AMU Q & A thread  
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? [Re: mister]
#14343329 - 04/24/11 12:34 PM (12 years, 10 months ago) |
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Quote:
mister said: It may just have run into an area of lower moisture. unless there is an area of green going on. Set it to the side and watch it. The sub may have also compacted and that could of made it harder for the mycelium to break through that area. throw out when it goes green for sure. Just watch and wait, but do not open in your house. Go outside, or pc them then discard to kill the mold, but this will stink.
Thanks, that was insightful. I suppose I'll just wait it out and see how it turns out in a few weeks. So if no green develops is it safe to eat off of?
I noticed when I PCd the jar with the green mold that when it was done the mold had turned grey, I junked the insides and washed the jar. And I never open the jars inside, especially anything with mold. 
I've done some very thorough reading for quite some time before I started this project. (2-3 years.) It's something I've been meaning to try for awhile and just got around to doing it.
What about the other two pictures, should I birth them? It's only been a little over a month since inoculation, but they've looked completely white for almost two weeks now. I haven't noticed any pinning, but I wasn't sure if I should birth them so I thought I would ask here.
The latter two were the first batch I did. The first photo is from my second batch.
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savage.renegade



Registered: 07/02/10
Posts: 986
Loc: Bay area
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Re: Mold or something else? [Re: mister]
#14343333 - 04/24/11 12:35 PM (12 years, 10 months ago) |
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Now you have to figure out why you got mold so you can fix it. Where is the mold? At the bottom? At the top near your barrier or your inoculation point? How did you sterilize the jars? How did you inoculate them?
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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The second batch is the only ones that got mold. I PC'd four of the jars for 90 Min. and pan boiled three others. The green mold came from one of the pan boiled jars. The other two are fine.
The grey area is from one of the PC'd jars. The other three appear to be fine.
Both the green mold and the grey (mold?) area were at the inoculation points. I think my sterilization is fine as far as the cooking part goes.
I used a glovebox to inoculate. I flame sterilized the needles with denatured alcohol and used 90% alcohol and gloves for everything. Everything but the jars was wiped down with alcohol as well.
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savage.renegade



Registered: 07/02/10
Posts: 986
Loc: Bay area
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Re: Mold or something else? [Re: thatsnice]
#14343489 - 04/24/11 01:23 PM (12 years, 10 months ago) |
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Usually mold shows up fast if it got in there before or when you inoculated. Ive only goten mold a couple times when i first started without a pc. So im not that experienced with it. If it showed up later on, maybe it got through your barrier. If its only one inoculation point maybe you just got unlucky. Did you flame the needle every jar or just once for all jars? Could be that the needle picked up spores in the air and got in that way. But in a glovebox that would be very unlucky.
The one that was panboiled probably wasnt sterilized properly i believe, also the fact that you had 2 different molds makes it seem that way.
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Yeah, I sterilized the needle in between each jar so I wouldn't cross contaminate.
That grey could have been there for awhile. The green was noticed about two weeks ago and that's when I handled it. Under the light (6000k) it's really hard to see the grey area. I just noticed that it looked different than the rest of the substrate last night and decided to post and ask.
It's cool though, I only lost two out of thirteen, so it's not a total loss for my first try. 
Do you think I should birth the cakes in the last picture?
annnd I just noticed a contam board so sorry for posting in here.
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savage.renegade



Registered: 07/02/10
Posts: 986
Loc: Bay area
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Re: Mold or something else? [Re: thatsnice]
#14343706 - 04/24/11 02:21 PM (12 years, 10 months ago) |
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Personally i wouldn't. Mold is hard to identify. Sometimes you need dna sequencing or a microscope. Post in contamination
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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So don't birth the other cakes? The bottom photos aren't from the same batch as the top and as far as I can tell have been fine. No signs of mold or anything abnormal.
Edited by thatsnice (04/24/11 02:23 PM)
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savage.renegade



Registered: 07/02/10
Posts: 986
Loc: Bay area
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Re: Mold or something else? [Re: thatsnice]
#14343728 - 04/24/11 02:28 PM (12 years, 10 months ago) |
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I would birth the ones without mold
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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♥
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mister
Nature nut



Registered: 10/04/08
Posts: 2,926
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Re: Mold or something else? [Re: thatsnice]
#14343806 - 04/24/11 02:46 PM (12 years, 10 months ago) |
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But on the topic of anything with mold on it. I would not try to ingest anything that came from a molded substrate. But that is just me.
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? [Re: mister]
#14392595 - 05/03/11 01:25 AM (12 years, 9 months ago) |
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Quote:
mister said: But on the topic of anything with mold on it. I would not try to ingest anything that came from a molded substrate. But that is just me.
Thanks for the help. It's been a week since I put the jar with the grey discoloration aside and it never turned green. it's just a little tiny grey blotch. is it mold, do any of you know if it could be safe to use?

updated pic.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Mold or something else? (moved) [Re: thatsnice]
#14395082 - 05/03/11 03:58 PM (12 years, 9 months ago) |
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This thread was moved from Mushroom Cultivation.
Reason: Moved at request of original poster.
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? (moved) [Re: RogerRabbit]
#14398346 - 05/04/11 05:05 AM (12 years, 9 months ago) |
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Thanks for the move R.R.
Can you help me or anyone else?
I did some digging around and I found this from a R.R. Notes thread:
*Mushrooms from a contaminated cake are safe to eat. They don't 'absorb' contaminants from the substrate or all wild mushrooms would be unsafe to eat. If the mold or bacteria is on the fruit itself of course, don't eat it. It's common in the commercial industry to harvest fruits from contaminated substrates and send them to market. There are no health regulations against it because it's not a risk factor.
So is it possible to open that jar and scrape that small grey space off the cake and use it?
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? (moved) [Re: thatsnice]
#14406057 - 05/05/11 04:15 PM (12 years, 9 months ago) |
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bump
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Mold or something else? (moved) [Re: thatsnice]
#14406705 - 05/05/11 06:36 PM (12 years, 9 months ago) |
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Looks like a bad spot, I'd toss the cake.
-------------------- You make it all possible. Doesn't it feel good?
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thatsnice
Good Sir



Registered: 02/04/11
Posts: 43
Last seen: 8 years, 8 months
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Re: Mold or something else? (moved) [Re: Doc_T]
#14408371 - 05/06/11 01:24 AM (12 years, 9 months ago) |
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thanks doc. :]
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