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 Astorescens
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Registered: 11/20/09
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 Making LC with Azurescens mycelium and alder chip broth 
#11819705 - 01/13/10 12:43 AM (14 years, 3 months ago) |
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I'm wondering if I can make a LC by simply adding mycelium to alder chip broth.
I have looked all over and all I can find is adding spores to make a LC.
BTW I'm using azurescens for this experiment.
It seems like it would work to me, but I would love input on this.
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Fahkface
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 Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#11819992 - 01/13/10 02:28 AM (14 years, 3 months ago) |
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Just try it!
As far as I know, no one has done it yet. At least not in this particular way. However, there have been people who have successfully transplanted pieces of Cube tissue to LC's.
The question is, weather the wood chip broth contains enough nutrients for the mushroom to be satisfied.
The liquid would have to be PC'd, though!
You should probably just try it. If it doesn't work, try to add some malt sugar etc. in. Maybe it'll work then.
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Astorescens
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Registered: 11/20/09
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Fahkface]
#11835602 - 01/15/10 02:37 PM (14 years, 3 months ago) |
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so i have done the baby bottle tek and also i have done it in 3 jars..
after the first day i noticed little mycelium growth.. so so far a good sign! hopefully this will work..
if i start to notice significant growth i will post the exact process i used.
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Magick
Thinker
Registered: 01/25/09
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#11836145 - 01/15/10 04:00 PM (14 years, 3 months ago) |
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There's no reason it shouldn't work - any live fungal tissue is able to be used for growing out the fungus - the key word here being 'live'. Once the mycelium is dried out or dead, it is dead.
Spores don't have to be alive on the other hand and can be used months or even years after they've been dropped.
Just follow the baby bottle tek and add the mycelium in the same manner as you would with spores - soak alder chips in water for 24+ hours, separate the wood and add a little karo corn syrup to the wood broth. Sterilize this (pressure cooking is recommended by many here, but I see nothing wrong with microwave sterilization although this is a source of great debate here!) broth and add your spores or live-tissue.
Growth can generally be seen in the first few days, and within 20 days the mycelium should be pretty-much grown out. A fellow mycologist once told me, be in the habit of not checking them 'too' often because every time you do you risk causing contamination. Limit yourself to every 2 or 3 days - you're looking for growth and for signs of contamination.
Contamination is generally more noticeable after you've transferred your mycelium to a solid substrate. A healthy cake will be mostly white, with the possibility of some light green/blue spots. It may appear slightly 'furry' and this is alright. A cake contaminated with bacterium will generally have green spots and smell sour - abit like a wet sock or a bad sponge (be careful smelling for mold, some is dangerous and if the contam is obvious just throw it out.) Molds can show up as white, green or black (black mold = IMMEDIATELY throw out!) Cobweb mold will appear as greyish white and will be more hairy (more 'cobweb' like) than actual mycelium.
Molds can represent a significant health risk - even after a moldy cake is thrown out if it was allowed to sit too long. When in doubt, throw it out! The good news is that cyanescens and azurescens are quite hardy fungi, and can beat some contaminations and still grow healthy (more or less) mushrooms.
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Fahkface
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Magick]
#11839527 - 01/16/10 03:51 AM (14 years, 3 months ago) |
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Quote:
A fellow mycologist once told me, be in the habit of not checking them 'too' often because every time you do you risk causing contamination. Limit yourself to every 2 or 3 days - you're looking for growth and for signs of contamination.
When it comes to LC's it doesn't matter how often you check them. The LC is supposed to be in a sterile environment anyway. You don't have to open anything to check for contams or growth, but see right through the jar, bottle, or whatever you cultivate it in.
When it comes to incubated substrates, that ain't in a sterile environment, this is true, though.
Quote:
Spores don't have to be alive on the other hand and can be used months or even years after they've been dropped.
Just for the records: Spores do have to be alive to be able to germinate.
The ability of germination is limited as well;)
Quote:
A cake contaminated with bacterium will generally have green spots
I don't really know of any bacterial that will cause green spots. The only thing that comes to my mind in terms of green spots is Trichoderma, which is a mold.
Now, just because I don't know, it doesn't mean it's not true. A source for this would be nice however
Generally, you're right. Contams can be a risk for your health and contaminated substrates or cakes should indeed be tossed as fast as possible, just to reduce the risk of heavy sporelation etc.
Again for the record: Fruits that grew from cakes or substrates, that suffered from a trichoderma infection are fine to eat, as long as the fruits themselves ain't contaminated. So if you have a trich problem, and on the other side of the cake fruits are growing, it's no problem to consume the fruits.
Just make sure, it really is trich!
Edited by Fahkface (01/16/10 03:59 AM)
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Astorescens
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Fahkface]
#11860279 - 01/19/10 03:14 PM (14 years, 2 months ago) |
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im not sure if you can see it verywell but there has been alot of growth in the LC.
 
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Magick
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#11867114 - 01/20/10 04:29 PM (14 years, 2 months ago) |
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Sure can't, but I believe you. It can be pretty hard to see inside an LC without a lot of direct light. Good luck 
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Astorescens
Hunter and gatherer
Registered: 11/20/09
Posts: 234
Loc: Silverton
Last seen: 2 years, 5 months
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Magick]
#11878436 - 01/22/10 11:52 AM (14 years, 2 months ago) |
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So I thought I'd mention I did not PC or boil my LC (the bottled one) and there is significant growth.. I guess we will see what happens after I inoculate..
I also have 3 half pint jars of LC (that I did boil) for experimenting.. One jar I open and air out every couple days (little growth) the second I poked a pencil size hole in the lid and stuffed it with fish tank filter material.(no noticable growth) and the third jar has been sealed since I put the mycelium in it. (most growth out of the jars) but the baby bottle has many times more growth than all the jars combined..
I will try to take some visible pictures asap.
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The hunter is now the hunted.
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Astorescens
Hunter and gatherer
Registered: 11/20/09
Posts: 234
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#11975873 - 02/06/10 02:49 PM (14 years, 2 months ago) |
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ok i think you can see the growth now...
this is what it looks like before i shake it up, every night it all bunches back up..
how much more should i let it grow before storing or use?
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TeamAmerica
Registered: 12/02/08
Posts: 2,954
Last seen: 10 years, 11 months
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#11975893 - 02/06/10 02:53 PM (14 years, 2 months ago) |
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The azures in your sig look awesome!
Ive always wanted to try to grow them...
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DryGrain
Psychonaut
Registered: 01/13/10
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: TeamAmerica]
#11993527 - 02/09/10 05:56 AM (14 years, 2 months ago) |
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Looks great!
Subscribed...
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Astorescens
Hunter and gatherer
Registered: 11/20/09
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: DryGrain]
#12047814 - 02/17/10 03:02 PM (14 years, 1 month ago) |
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so i have a question... whats the most effective way to spread an LC outdoors? should i just inoculate wood chips and then transfer outdoors? or can i just spread the LC?
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 badman
Registered: 06/14/06
Posts: 4,039
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#12047841 - 02/17/10 03:05 PM (14 years, 1 month ago) |
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Id do the woodchips
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Dbb5
Registered: 10/25/09
Posts: 486
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: badman]
#12047883 - 02/17/10 03:08 PM (14 years, 1 month ago) |
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You can use your lc whenever you want.
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murrtheblur
Chillin Chillin
Registered: 10/20/09
Posts: 1,314
Loc: the stix
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Astorescens]
#12048052 - 02/17/10 03:33 PM (14 years, 1 month ago) |
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I was wonder something similar, I plan to make wood-lover lc and inoculate some of the rotted stumps around my yard. Does anyone have experience with this or something like it?
~blur~
btw i was planing on using big laughing gyms for this project cuz they're natural here
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: murrtheblur]
#12048296 - 02/17/10 04:06 PM (14 years, 1 month ago) |
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How bout an outdoor bed/ patch?
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DryGrain
Psychonaut
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Last seen: 13 years, 10 months
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: badman]
#12051334 - 02/17/10 11:41 PM (14 years, 1 month ago) |
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Is it a good idea to start your LC on grain or cardboard first, and then make spawn for your outdoor wood chip bed?
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Fahkface
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: DryGrain]
#12051649 - 02/18/10 01:06 AM (14 years, 1 month ago) |
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Quote:
I plan to make wood-lover lc and inoculate some of the rotted stumps around my yard
I'm afraid you won't be successful doing that.
Wood loving Psilocybes need fresh wood chips, preferably hard wood. A rotted stump is streaked by other cultures, which is why it's rotten.
Go for a bed of hard wood chips instead and you'll definitely be more successful!
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murrtheblur
Chillin Chillin
Registered: 10/20/09
Posts: 1,314
Loc: the stix
Last seen: 7 years, 2 months
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Re: Making LC with Azurescens mycelium and alder chip broth [Re: Fahkface]
#12051788 - 02/18/10 02:14 AM (14 years, 1 month ago) |
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Thank you both for answering my question, I will take that into mind. I hope it helps the OP as well.
~blur~
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