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 gribi
Mushroom Eater
Registered: 08/20/08
Posts: 89
Last seen: 12 years, 5 months
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 Waking up A.blazei spores 
#11554671 - 11/30/09 12:12 PM (14 years, 4 months ago) |
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In late October, I put some A.blazei spores on MYPA agar. They did not germinate, so I scoured this board for some ideas on what to do and a few people suggested putting other growing culture on the same plate. So, I went to the store and got some nice looking button mushrooms (A. bisporus) and put some of their inner cap tissue on the same plate.
For a while, it looked like A. bisporus pieces died (they turned brown/black), but after a few weeks, they started putting out some nice cottony mycelium. This was a nice surprise.
But I was in for an even bigger surprise. A few weeks ago, another area of the plate started growing - a place where the A. bisporus didn't touch. Could it be A.blazei spores waking up?
I isolated a section of this new growth and put it on a new plate. Here it is, as of today:
Any thoughts/comments on this? Is it likely to be A.blazei? Or is it something else (a ninja piece of A. bisporus that jumped around the plate? a contaminant? something else?)
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I am looking for the following edible cultures:
- Chicken of the woods (Laetiporus sulphureus)
- Wood blewit (Lepista nuda)
- Cauliflower (Sparassis crispa)
My trade list of available cultures is in my Journal.
Edited by gribi (11/30/09 12:14 PM)
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fungus_tao
Hah Zah!
Registered: 05/10/06
Posts: 1,856
Loc: 
Last seen: 6 years, 6 months
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Re: Waking up A.blazei spores [Re: gribi]
#11554703 - 11/30/09 12:19 PM (14 years, 4 months ago) |
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Looks like success to me. Great job!! I am currently trying to revive my a. blazei culture.
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Follow the light
The Light is your guide.
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houdinihar
Adventurous Learner
Registered: 09/12/08
Posts: 1,111
Last seen: 4 years, 8 days
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Re: Waking up A.blazei spores [Re: fungus_tao]
#11558373 - 11/30/09 09:12 PM (14 years, 4 months ago) |
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great success.
houdinihar
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin 
Last seen: 6 months, 7 days
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Re: Waking up A.blazei spores [Re: gribi]
#11560644 - 12/01/09 06:23 AM (14 years, 4 months ago) |
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Congratulations, that's how you do it.
Carsten
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Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,090
Loc: Texas
Last seen: 5 years, 2 months
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Re: Waking up A.blazei spores [Re: Mycelio]
#11560878 - 12/01/09 08:07 AM (14 years, 4 months ago) |
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I have some Leucoagaricus americanus and Agaricus campestris spores that I've been trying to germinate with no success. I'll have to give this a try!
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
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 Jef
Out-of-work Sex Slave
Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
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Re: Waking up A.blazei spores [Re: Paresthesia]
#11564034 - 12/01/09 04:30 PM (14 years, 4 months ago) |
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Good for you Gribi!
Quote:
Paresthesia said:
I have some Leucoagaricus americanus and Agaricus campestris spores that I've been trying to germinate with no success. I'll have to give this a try!
I believe you can try your Chlorophyllum rhacodes for the leucoagaricus americanus. That would be the most similar.
Jef
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I am my own lab rat.
Tell me and I will forget. Show me and I will remember. Involve me and I will learn.
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RonPaulVerm
Piss Christ
Registered: 07/21/08
Posts: 1,072
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Re: Waking up A.blazei spores [Re: Jef]
#11564086 - 12/01/09 04:40 PM (14 years, 4 months ago) |
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gribi, did you swipe the spores on that part of the plate?
Did you put the A. Bisporus tissue on a section that was swiped by your A Blezei spores?
The tissued section seems to be overlapping the new growth a little and the new growth is a bit thinner leading me to think it's growth from spores....could be wrong though.
I will watch.
There's only 1 way to find out.... 
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Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,
Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have
Primitive Skills
Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms
...I DO advocate learning about
Law Enforcement Against Prohibition
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gribi
Mushroom Eater
Registered: 08/20/08
Posts: 89
Last seen: 12 years, 5 months
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Re: Waking up A.blazei spores [Re: RonPaulVerm]
#11564254 - 12/01/09 05:03 PM (14 years, 4 months ago) |
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Quote:
RonPaulVerm said:
gribi, did you swipe the spores on that part of the plate?
I used a spore syringe (i.e. spores in sterile water) - putting a few droplets on the plate.
Quote:
Did you put the A. Bisporus tissue on a section that was swiped by your A Blezei spores?
I can't say for sure, because by the time I put A. Bisporus tissue on the plate, the droplets have dried out. I believe it was not in the same spots though (that would make for some pretty odd coincidence).
Quote:
The tissued section seems to be overlapping the new growth a little and the new growth is a bit thinner leading me to think it's growth from spores....could be wrong though.
I will watch.
It looks that way to me too. New growth seems to stop at the border of the existing mycelial mat of A. bisporus.
Quote:
There's only 1 way to find out....
Exactly. It will be a while, but I'll update this post with my progress. Also, I should be able to detect the presence (or absence) of the almond smell on spawn.
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I am looking for the following edible cultures:
- Chicken of the woods (Laetiporus sulphureus)
- Wood blewit (Lepista nuda)
- Cauliflower (Sparassis crispa)
My trade list of available cultures is in my Journal.
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gribi
Mushroom Eater
Registered: 08/20/08
Posts: 89
Last seen: 12 years, 5 months
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Re: Waking up A.blazei spores [Re: gribi]
#11597401 - 12/06/09 04:28 PM (14 years, 4 months ago) |
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Quick update: it grew out nicely, so it's time to put it on grain and see what it really is.
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I am looking for the following edible cultures:
- Chicken of the woods (Laetiporus sulphureus)
- Wood blewit (Lepista nuda)
- Cauliflower (Sparassis crispa)
My trade list of available cultures is in my Journal.
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Jef
Out-of-work Sex Slave
Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
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Re: Waking up A.blazei spores [Re: gribi]
#11599802 - 12/06/09 10:20 PM (14 years, 4 months ago) |
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That's some fine looking growth !
Good growing,
Jef
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I am my own lab rat.
Tell me and I will forget. Show me and I will remember. Involve me and I will learn.
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