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Offlinebeto
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Orissa India - My first attempt growing shrooms!!
    #11121020 - 09/24/09 06:37 PM (14 years, 6 months ago)

STRAIN: Orissa India
METHOD: PF TEK - CASING - LIQUID CULTURE
BEGINNING: 09/16
STATUS: STILL IN PROGRESS

* 09/15 - Got the spore print! Made a 20cc syringe using 1/4 of a coin sized print. I dropped some distilled water on the print and using a inoculation ring, i gently stirred until the spores were all loose, then i poured the solution inside a sterile urine collection pot. Then i poured about 20ml of distilled water and mixed well. I sucked the solution into syringe and put it into the fridge and left it there for 48h for rehydration.

* 09/17 - I prepared the substrate (pf tek recipe plus tweaking) filled the half pint mason jars and PC for 60min. I'll let them cooling down overnight.

* 09/18 - Just inoculated the jars using 0,5cc per hole (4 holes in each jar). Total = 8 jars. Did all procedures inside a sterilised glovebox, flaming needle etc. Then i put all jars inside a styrofoam box. Inside the box was a 2 liters plastic bottle full of water with an aquarium thermostat/heater set for 84F.

* 09/24 - No signs of mycelium yet. To entertain myself i built a magnetic stirrer using a computer fan and a HD magnet. Also prepared a liquid culture using water and organic honey, wich i inoculated with the rest of the spore solution.


STAY TUNED FOLKS!! Send me positive vibrations!!


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Offlineveda_sticks
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Re: Orissa India - My first attempt growing shrooms!! [Re: beto]
    #11121047 - 09/24/09 06:42 PM (14 years, 6 months ago)

nice job, remove the foil on your jars. its only needed for keeping water out during sterilisation.

I wouldnt bother casing, just frit them as cakes in a shotgun terrarium. Casing is only realy helpfull when fruiting straight grains that dont hold much moisture.

Nice stir plate, ive always want to build 1


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblefeelfunny
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Re: Orissa India - My first attempt growing shrooms!! [Re: veda_sticks]
    #11121061 - 09/24/09 06:43 PM (14 years, 6 months ago)

if you case spawn you will get more that way caseing with out it is just not going to out preform a cake


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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OfflineCliffo
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Re: Orissa India - My first attempt growing shrooms!! [Re: feelfunny]
    #11121257 - 09/24/09 07:17 PM (14 years, 6 months ago)

Your stirring thing is awesome *steals idea*


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Invisiblefeelfunny
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Re: Orissa India - My first attempt growing shrooms!! [Re: Cliffo]
    #11121296 - 09/24/09 07:28 PM (14 years, 6 months ago)

i just got a kit to make one for 12$ hope it was worth it


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlinebeto
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Re: Orissa India - My first attempt growing shrooms!! [Re: veda_sticks]
    #11121313 - 09/24/09 07:31 PM (14 years, 6 months ago)

Quote:

veda_sticks said:
nice job, remove the foil on your jars. its only needed for keeping water out during sterilisation.

I wouldnt bother casing, just frit them as cakes in a shotgun terrarium. Casing is only realy helpfull when fruiting straight grains that dont hold much moisture.

Nice stir plate, ive always want to build 1





Thx bro i'll remove the foil! The reason i'll prepare a bulk substrate it's because orissa india don't do well in cakes, this strain is a hungry monster, needs lots of nutrients. I'll prepare a bulk substrate mixing coco coir (i think it's called like this, its coconut shell dust) and coffee grounds. After it's colonized, i'll cover with a casing layer made of peat moss and vermiculite (with gypsum and calcium cabonate). I think this will help my children to grow nicely!!! I would have started with rye grain, but since i had the ingredients for the PF cakes and i was in sush a rush to grow the babies, i couldn't wait!! lol

Stay tuned!

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Offlinebeto
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Re: Orissa India - My first attempt growing shrooms!! [Re: feelfunny]
    #11121322 - 09/24/09 07:33 PM (14 years, 6 months ago)

Quote:

feelfunny said:
i just got a kit to make one for 12$ hope it was worth it





Killer man!! I hope it's worth it too!! You see, i had all the materials here laying around... A damaged HD, old computer fan, wood... I made it out of junk! LOL But its working fine!! I just need to buy a potentiometer to control the speed!

Stay tuned!

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Invisiblefeelfunny
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Re: Orissa India - My first attempt growing shrooms!! [Re: beto]
    #11121326 - 09/24/09 07:34 PM (14 years, 6 months ago)

sound like you know what you are doing cant wait too see the end.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlinesamossa
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Re: Orissa India - My first attempt growing shrooms!! [Re: feelfunny]
    #11121449 - 09/24/09 07:58 PM (14 years, 6 months ago)

Looks cool!

Can one of you guys explain the magnetic stirrer and the liquid culture...
Was it that for?

Thx

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Invisiblefeelfunny
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Re: Orissa India - My first attempt growing shrooms!! [Re: samossa]
    #11121464 - 09/24/09 08:01 PM (14 years, 6 months ago)

to keep the LC mixing to promote faster growth


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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OfflinebioBot

Registered: 05/02/09
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Re: Orissa India - My first attempt growing shrooms!! *DELETED* [Re: veda_sticks]
    #11122776 - 09/24/09 11:06 PM (14 years, 6 months ago)

Post deleted by bioBot

Reason for deletion: ...


Edited by bioBot (01/02/14 05:51 AM)

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Offlinebeto
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Re: Orissa India - My first attempt growing shrooms!! [Re: bioBot]
    #11131131 - 09/26/09 12:30 PM (14 years, 6 months ago)

26/09 - Finally after 8 days since inoculation, at least 1 of the 8 jars is showing signs of biological activity. I think it's mycelium, it's white. Too soon to tell. Thanks God it's not green!




Stay tuned folks!

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Offlinescrotesmcgrotes
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Re: Orissa India - My first attempt growing shrooms!! [Re: beto]
    #11131158 - 09/26/09 12:35 PM (14 years, 6 months ago)

keep it up!  love the ingenuity on the stirrer

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Offlinesnesgamer
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Re: Orissa India - My first attempt growing shrooms!! [Re: scrotesmcgrotes]
    #11131197 - 09/26/09 12:42 PM (14 years, 6 months ago)

You should do a  tek on making the stirrer.

That would be interesting.

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OfflineDiscGolfer
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Re: Orissa India - My first attempt growing shrooms!! *DELETED* [Re: snesgamer]
    #11134787 - 09/26/09 11:55 PM (14 years, 6 months ago)

Post deleted by DiscGolfer

Reason for deletion: .



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Offlinebeto
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Re: Orissa India - My first attempt growing shrooms!! [Re: DiscGolfer]
    #11141871 - 09/28/09 05:23 AM (14 years, 5 months ago)

09/28 - That only jars seems promising! The mycelium is growing slow and it's getting more white. The other jars still stalled. The weird thing is they germinated only in one point. Maybe the print i got was fucked up.


Edited by beto (09/28/09 05:23 AM)

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OfflineHarold Hole
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Re: Orissa India - My first attempt growing shrooms!! [Re: beto]
    #11141896 - 09/28/09 05:31 AM (14 years, 5 months ago)

Wow, not to be negative but for 10 days thats pretty slow colonization


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Offlinebeto
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Re: Orissa India - My first attempt growing shrooms!! [Re: Harold Hole]
    #11141950 - 09/28/09 05:57 AM (14 years, 5 months ago)

Quote:

hinderpits said:
Wow, not to be negative but for 10 days thats pretty slow colonization





Yeah... But from what i've been reading, this strain colonizes really slow. And as i said, maybe my print was too old or fucked up or sumthing... dunno, i have to wait to see!! But if until next week the other jars don't show any signs of life, i'm throwing all away.

Stay tuned!

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Offlinethcisme
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Re: Orissa India - My first attempt growing shrooms!! [Re: Harold Hole]
    #11142017 - 09/28/09 06:42 AM (14 years, 5 months ago)

[empty]

Edited by thcisme (11/15/09 07:33 AM)

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OfflineMonkeycyst
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Re: Orissa India - My first attempt growing shrooms!! [Re: beto]
    #11142087 - 09/28/09 07:11 AM (14 years, 5 months ago)

Quote:


* 09/17 - I prepared the substrate (pf tek recipe plus tweaking) filled the half pint mason jars and PC for 60min. I'll let them cooling down overnight.




By 'tweaking', you mean you used whole grain white rice instead of brown rice flour?


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