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Offlineshivathedestroyer
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anyone figured out how to do the sterile needle/sponge trick with pf jars?
    #11091142 - 09/19/09 07:51 PM (14 years, 6 months ago)

i was just admiring this post from citric:

http://www.shroomery.org/forums/showflat.php/Number/10636495

and wondered if this could be used with a pf jar?


i figure you'd get alcohol dripping down from the sponge? maybe im thinking too much alcohol?


could you make self healing pf lids?


any tips would be cool.


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Invisiblefeelfunny
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: shivathedestroyer]
    #11091181 - 09/19/09 07:59 PM (14 years, 6 months ago)

you cant  use self healing lids the myc needs to breath


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: feelfunny]
    #11091184 - 09/19/09 07:59 PM (14 years, 6 months ago)

oh and just don't use to much alcohol and it wont drip


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlineveda_sticks
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: feelfunny]
    #11091230 - 09/19/09 08:07 PM (14 years, 6 months ago)

Quote:

feelfunny said:
you cant  use self healing lids the myc needs to breath




yes you can. If you use self healing injection sites, you will need an extra hole with a filter for gas echange.

a 1/4 inch hole in the middle should be plenty for gas exchange. The advantage of this aswell is you dont need a dry verm layer.

I should my mate this and its all hes using now for pf cakes, 4 self helaing injkectings sites and a micropore taped hole in the middle.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisiblefeelfunny
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: veda_sticks]
    #11091265 - 09/19/09 08:11 PM (14 years, 6 months ago)

that would be modified from the tek the lid by its self is not going to work. i agree that if you change it from the tek it will work but ether way it has to be modified.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlineshivathedestroyer
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: feelfunny]
    #11093002 - 09/20/09 01:42 AM (14 years, 6 months ago)

i had kinda figured to add air exchange, i heard tyvek is good for this?


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Offlineoobajoob
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: shivathedestroyer]
    #11093013 - 09/20/09 01:46 AM (14 years, 6 months ago)

i wouldnt fuck with it. the simple fact that this isnt the 100% accepted method for innoculating pf jars, it obviously isnt as good as the good ol' dry verm barrier and holes in the lid.

like i always say, if it aint broke, dont fix it. :smile:

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OfflineNoOneKnowsHowToAct
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: oobajoob]
    #11093022 - 09/20/09 01:49 AM (14 years, 6 months ago)

my lids have 2 rtv silicone ports and a 1 1/2" tyvek discs for "breathing". Instead of a sponge i just flamed the needle and used a paper towel soaked in alcohol. other than that difference I did pretty much the same thing. I'm a noob. I only inoculated 6 jars. I didn't have any contams. WBS but it shouldn't matter.


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Invisiblesubject
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: NoOneKnowsHowToAct]
    #11093426 - 09/20/09 05:28 AM (14 years, 6 months ago)

dry verm and a simple layer of aluminum foil haven't steered me wrong yet with pf..


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Offlinedstark
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: subject]
    #11093502 - 09/20/09 06:13 AM (14 years, 6 months ago)

Yes i use it on PF tek to :yesnod:


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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: dstark]
    #11096406 - 09/20/09 06:34 PM (14 years, 6 months ago)

If you wanted to make lids like mine for PF jars, you would have to modify it.  Here is a shitty paint job.




The red dots are your injection ports, and the middle would be just like on the original lid.  A cut out with tyvek silicone.  This would also give you a LITTLE bit more substrate, as you would not need a dry verm layer.

I have not tested this as I have only done PF jars once in my life.


--------------------
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Offlinetektonic
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: shivathedestroyer]
    #11096712 - 09/20/09 07:34 PM (14 years, 6 months ago)

Quote:

shivathedestroyer said:
i was just admiring this post from citric:

http://www.shroomery.org/forums/showflat.php/Number/10636495

and wondered if this could be used with a pf jar?


i figure you'd get alcohol dripping down from the sponge? maybe im thinking too much alcohol?


could you make self healing pf lids?


any tips would be cool.





i use it with self healing lc's and pf tek jars

alc drips into the jar, unless you squeeze most of the alc out

i try to not get alc in my jars, but it does, and the jars are fine


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Offlinefundamentalchair
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: tektonic]
    #11097008 - 09/20/09 08:37 PM (14 years, 6 months ago)

I just dipped the tip of the needle in a shot glass filled with 90% IPA. Seemed to work out for me, although that's the only time I shot spores.


--------------------
semper necessitas probandi incumbit ei qui agit


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Offlineshivathedestroyer
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: fundamentalchair]
    #11101030 - 09/21/09 03:14 PM (14 years, 6 months ago)

im so worried about contams i'd still use the dry verm layer.


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Offlinefundamentalchair
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: shivathedestroyer]
    #11101751 - 09/21/09 05:28 PM (14 years, 6 months ago)

I thought you were just looking for a way to cool the needle down before injecting. I know I have to do something to keep the poly fill from melting to my injection port.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light

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Offlineveda_sticks
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: fundamentalchair]
    #11101774 - 09/21/09 05:33 PM (14 years, 6 months ago)

why would you even want to??

glovebox
ipa cleaned gloved hands
flame needle red hot
leave cool for 10 seconds
inject, oh no a little sissle my spores are dying!!
the rest flows fine
job done

90% IPA isnt as effective as 70%, besides alcohol is a sanitiser.



--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

Edited by veda_sticks (09/21/09 05:34 PM)

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Invisiblefeelfunny
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: veda_sticks]
    #11101783 - 09/21/09 05:35 PM (14 years, 6 months ago)

its officially veda has gone on a  thread closing spree almost glad you cant


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlinefundamentalchair
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Re: anyone figured out how to do the sterile needle/sponge trick with pf jars? [Re: feelfunny]
    #11101901 - 09/21/09 05:57 PM (14 years, 6 months ago)

When shooting spores, I didn't have a glovebox at the time. Also, I use polyfill for gas exchange and hot needles melt the poly.

While 70% is better for sanitizing due to the water keeping it around longer, I was just using it as a sterile environment to cool my needle down. 90% didn't burst into flames, so it seemed to get the job accomplished.

I dilute my 90 down to 60-70 when using it as a sanitizer but use the 90 in my lamp, btw.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light

Extras: Filter Print Post Top
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