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OfflineAdonisComplex
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Registered: 09/08/09
Posts: 30
Last seen: 12 years, 10 months
Vacutainers melting during inoculation?
    #11032811 - 09/10/09 07:36 AM (14 years, 6 months ago)

When using red-top vacutainers as a self-healing injection port on the lids of sub jars, what is the appopriate method of syringe sterilization?  If the needle is flamed until red hot, will this melt or damage the vacutainer upon insertion?  Tyvek has a tendency to melt when inoculating with a red-hot needle.

Should the needle be flamed, then a few droplets expelled prior to inoculation, and be swabbed with alcohol to cool the syringe?  RR has stated that flaming is best, and using an alcohol swab is like washing your dishes with sewer water.

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OfflineDamnskippi
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Registered: 09/08/09
Posts: 124
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Re: Vacutainers melting during inoculation? [Re: AdonisComplex]
    #11032818 - 09/10/09 07:39 AM (14 years, 6 months ago)

Im no expert but as far as i know u are supposed to give the needle a chance to cool before inoculation. Otherwise the heat will destroy the spores. shouldnt take more then like 30 seconds though.


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"When one person suffers from a delusion it is called insanity; when many people suffer from a delusion it is called religion."

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InvisibleDoc_T
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Registered: 03/06/09
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Re: Vacutainers melting during inoculation? [Re: Damnskippi]
    #11032856 - 09/10/09 07:56 AM (14 years, 6 months ago)

Just squirt a drop out.


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You make it all possible. Doesn't it feel good?

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OfflineAdonisComplex
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Registered: 09/08/09
Posts: 30
Last seen: 12 years, 10 months
Re: Vacutainers melting during inoculation? [Re: Doc_T]
    #11032884 - 09/10/09 08:08 AM (14 years, 6 months ago)

Boxxy!!  My queen!

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Offlinecloudsaregathering
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Registered: 08/08/09
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Re: Vacutainers melting during inoculation? [Re: AdonisComplex]
    #11033352 - 09/10/09 10:28 AM (14 years, 6 months ago)

I always just wipe the needle with alcohol cools it right off...


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"the root of the problem has been isolated"

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Offlinemilkman59
milkman
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Registered: 02/01/09
Posts: 267
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Re: Vacutainers melting during inoculation? [Re: cloudsaregathering]
    #11033399 - 09/10/09 10:41 AM (14 years, 6 months ago)

Quote:

cloudsaregathering said:
I always just wipe the needle with alcohol cools it right off...




agreed


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milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09

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