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Offlinejust me
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cloning an isolate to rejuvinate ?
    #10696162 - 07/17/09 07:16 PM (14 years, 8 months ago)

so i was working with an isolate

i ran out of genetics...and didnt realize till the tub had almost finished flushing that i wanted to keep this one alive...

so i took tissue samples from two small(not fully mature) mushrooms at harvest time.

instead of taking tissue samples from the giants from the tub.

i put the tissue on agar and there was no sectoring...

now i have some nice clean LC's of said genetics

what im wondering is, because i took tissue from smaller later bloomers, is this trait going to carry on into fruiting? or is it all the same all the same? will future flushes produce like previous flushes.


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OfflineCuriousGeorge83
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Re: cloning an isolate to rejuvinate ? [Re: just me]
    #10696189 - 07/17/09 07:20 PM (14 years, 8 months ago)

Yes, I wonder the same thing.  It is my understanding though that if it is a true isolate, whichever fruit you clone would be genetically identical.


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Offlinejust me
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Re: cloning an isolate to rejuvinate ? [Re: CuriousGeorge83]
    #10696594 - 07/17/09 08:46 PM (14 years, 8 months ago)

is this true?


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OfflineCascadian
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Re: cloning an isolate to rejuvinate ? [Re: just me]
    #10697015 - 07/17/09 10:09 PM (14 years, 8 months ago)

Quote:

if it is a true isolate, whichever fruit you clone would be genetically identical


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Offlinejust me
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Re: cloning an isolate to rejuvinate ? [Re: Cascadian]
    #10697025 - 07/17/09 10:11 PM (14 years, 8 months ago)

:hug:

thanks guys/gals

...ur surrrrrrrrrre?


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OfflineAgarAgar
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Re: cloning an isolate to rejuvinate ? [Re: just me]
    #10697171 - 07/17/09 10:51 PM (14 years, 8 months ago)

so i was working with an isolate

i ran out of genetics...and didnt realize till the tub had almost finished flushing that i wanted to keep this one alive...

so i took tissue samples from two small(not fully mature) mushrooms at harvest time.

instead of taking tissue samples from the giants from the tub.

i put the tissue on agar and there was no sectoring...
(of course, it was a clone, you would never get sectoring.)

now i have some nice clean LC's of said genetics

what im wondering is, because i took tissue from smaller later bloomers, is this trait going to carry on into fruiting? or is it all the same all the same? will future flushes produce like previous flushes.
was the original spawn made from multispore, or from a clone?

-AgarAgar


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InvisibleJitsu
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Re: cloning an isolate to rejuvinate ? [Re: AgarAgar]
    #10697217 - 07/17/09 11:01 PM (14 years, 8 months ago)

Quote:

AgarAgar said:
so i was working with an isolate

i ran out of genetics...and didnt realize till the tub had almost finished flushing that i wanted to keep this one alive...

so i took tissue samples from two small(not fully mature) mushrooms at harvest time.

instead of taking tissue samples from the giants from the tub.

i put the tissue on agar and there was no sectoring...
(of course, it was a clone, you would never get sectoring.)

now i have some nice clean LC's of said genetics

what im wondering is, because i took tissue from smaller later bloomers, is this trait going to carry on into fruiting? or is it all the same all the same? will future flushes produce like previous flushes.
was the original spawn made from multispore, or from a clone?

-AgarAgar





clones sector all the time :wink:


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Offlinejust me
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10697376 - 07/17/09 11:39 PM (14 years, 8 months ago)

yup :wink:

agaragar, the original original was multispore, but my first go with it, it was already an a clone


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"The Downfall of Mankind; is Believing He Has Limitations."

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OfflineAgarAgar
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10698732 - 07/18/09 10:01 AM (14 years, 8 months ago)

no.... Clones do NOT sector.

Sectoring is caused by different types of mycelium, and a clone is a monoculture so, NO, clones do not sector.


-Graham


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OfflineStealthgrower
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Re: cloning an isolate to rejuvinate ? [Re: AgarAgar]
    #10698821 - 07/18/09 10:29 AM (14 years, 8 months ago)

Quote:

AgarAgar said:
no.... Clones do NOT sector.

Sectoring is caused by different types of mycelium, and a clone is a monoculture so, NO, clones do not sector.


-Graham


Actually, clones will sector, however a cloned true ISOLATE will not as far as I know.


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Infea said:
You ever seen that movie Constantine.  Well they pass into hell several times.  In hell there are melting human bodies, in your tub are melting mushroom bodies.  Your tub had passed into mushroom hell.

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InvisibleJitsu
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Re: cloning an isolate to rejuvinate ? [Re: AgarAgar]
    #10698845 - 07/18/09 10:34 AM (14 years, 8 months ago)

Quote:

Stealthgrower said:
Quote:

AgarAgar said:
no.... Clones do NOT sector.

Sectoring is caused by different types of mycelium, and a clone is a monoculture so, NO, clones do not sector.


-Graham


Actually, clones will sector, however a cloned true ISOLATE will not as far as I know.


Quote:

AgarAgar said:
no.... Clones do NOT sector.

Sectoring is caused by different types of mycelium, and a clone is a monoculture so, NO, clones do not sector.


-Graham




I'm sorry my friend but you are mistaken on this. The only way a clone will be a true isolate is if you are growing from an isolate.


It's fine to pull the tissue from a later flush.


--------------------
Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

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Edited by Jitsu (07/18/09 10:36 AM)

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OfflineAgarAgar
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10706647 - 07/19/09 10:24 PM (14 years, 8 months ago)

You are mistaken. A clone is an isolate, it is only one type of mycelium. Do yourself a favor and do some reading.

I have taken several biology college classes including cell biology and microbiology, and I know how culturing works and how to obtain pure cultures and isolates/clones. Cloning is a very easy way to obtain an isolate that will not sector because it is a monoculture.

I think you are mistaken as to how sectoring looks.

AgarAgar


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Offlineveda_sticks
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Re: cloning an isolate to rejuvinate ? [Re: AgarAgar]
    #10706743 - 07/19/09 10:39 PM (14 years, 8 months ago)

Quote:

AgarAgar said:
You are mistaken. A clone is an isolate, it is only one type of mycelium. Do yourself a favor and do some reading.

I have taken several biology college classes including cell biology and microbiology, and I know how culturing works and how to obtain pure cultures and isolates/clones. Cloning is a very easy way to obtain an isolate that will not sector because it is a monoculture.

I think you are mistaken as to how sectoring looks.

AgarAgar




Theres some confusion in this thread.

Cloning a fruit from a multispore grow will not nessesarily produce an isolate. Its still possible for a tissue sample from a multispore fruit to sector. Fruitbodys can infact be made up from severain strains of mycelium.

However, if you fruit grown was from an isolate and that was cloned, it would not sector since its already been isolated.

So if you cloned a fruit that has already been used from an isolate, that clone will produce the same results.

Be carefull not to go too far otherwise you will run into senesence. Doing it once may be OK to save the strain.

If growth characteristics change and performance suffers then the mycelium may be getting old.


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InvisibleJitsu
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Re: cloning an isolate to rejuvinate ? [Re: AgarAgar]
    #10706852 - 07/19/09 10:58 PM (14 years, 8 months ago)

Quote:

AgarAgar said:
You are mistaken. A clone is an isolate, it is only one type of mycelium. Do yourself a favor and do some reading.

I have taken several biology college classes including cell biology and microbiology, and I know how culturing works and how to obtain pure cultures and isolates/clones. Cloning is a very easy way to obtain an isolate that will not sector because it is a monoculture.

I think you are mistaken as to how sectoring looks.

AgarAgar




There are hundreds of active organisms inside a mushroom fruitbody. Strains will often come together to form the fruitbody. I'm not doubting your knowledge in biology but this is mycology my friend. I have seen many clones sector and if you do a bit of reading on this website there is plenty of information to back my statement up.


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Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

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OfflineCascadian
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10707348 - 07/20/09 12:43 AM (14 years, 8 months ago)

Quote:

Jitsu said:
Quote:

AgarAgar said:
You are mistaken. A clone is an isolate, it is only one type of mycelium. Do yourself a favor and do some reading.

I have taken several biology college classes including cell biology and microbiology, and I know how culturing works and how to obtain pure cultures and isolates/clones. Cloning is a very easy way to obtain an isolate that will not sector because it is a monoculture.

I think you are mistaken as to how sectoring looks.

AgarAgar




There are hundreds of active organisms inside a mushroom fruitbody. Strains will often come together to form the fruitbody. I'm not doubting your knowledge in biology but this is mycology my friend. I have seen many clones sector and if you do a bit of reading on this website there is plenty of information to back my statement up.


:haha:  :imwithstupid:

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InvisibleJitsu
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Re: cloning an isolate to rejuvinate ? [Re: Cascadian]
    #10708271 - 07/20/09 08:26 AM (14 years, 8 months ago)

"It's been shown from DNA testing that up to 200 organisms can be active in a single fruiting body.  If you're growing from multispore inoculation, several substrains can be active in a fruit.  If you're growing from a single sector isolate proved on agar in a petri dish, your clones will be isolated strains, but further down the cell division pool than the original petri dish, which is what you'd want to use for further growing.
RR"


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Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

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Offlinejust me
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10713024 - 07/21/09 01:53 AM (14 years, 8 months ago)

thanks


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InvisiblePinback
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Re: cloning an isolate to rejuvinate ? [Re: Jitsu]
    #10713090 - 07/21/09 02:28 AM (14 years, 8 months ago)

Where can one read about this testing?

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Offlinelastlokean
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Re: cloning an isolate to rejuvinate ? [Re: Pinback]
    #10713442 - 07/21/09 06:03 AM (14 years, 8 months ago)

Clone a multispore fruit onto a petri dish and watch it sector...


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How a Lokean makes chocolates

For a fully enclosed gh with a coolmist piped in, one may find that adding one of these to the opposite end as the intake will seriously help reduce pressure buildup and strain on the poor weak coolmist motor, while also dramatically improving fae without cutting polyfill stuffed slits all over the damn thing.  (That looks suspicious :smirk:)

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Re: cloning an isolate to rejuvinate ? [Re: lastlokean]
    #10713532 - 07/21/09 07:04 AM (14 years, 8 months ago)

Quote:

lastlokean said:
Clone a multispore fruit onto a petri dish and watch it sector...




exactly, you dont need dna testing to tell you that a multispore clone to tell you that the fruits are made up of multiple sets of ginetics.

the testing that was done was just the icing on the cake that gave further insight into how far it goes.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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