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Offlineblazeup420
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Registered: 04/30/09
Posts: 133
Last seen: 13 years, 7 months
couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!?
    #10296101 - 05/06/09 10:13 AM (14 years, 10 months ago)

im tryin pf tek for my first time growing i made a glove box out of a shelf wiped it down with alcohol and put plastic with slits in it for my hands i flamed the needle or wiped it down with alc after each jar and wiped the jar lidds with alc to (did ten) didnt have a pressure cooker so i boiled the substrate for about 90 min.i was just wondering if you think theyl b contamed or not and any extra steps i can take to b sterile...and do you leave the tin foil on the jars or put tap or just leave the holes exsposed cuz ive heard of each?thanx

and incubator not nessisary?i have the jars in the box they came in.. wrapped up in a big paper bag on a shelf...theres a curtain over the front of the shelf and a halogen flood lite pointed up at an angle for heat...is that good


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OfflineTunesy
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296116 - 05/06/09 10:17 AM (14 years, 10 months ago)

Get a nice thermometer, and check the temperature, anything above about 21C and you will see growth, You can leave the foil lids on with the holes exposed, as long as you have a layer of verm as a contam barrier. Make sure you don't get it too hot.

Tunes

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OfflineHarlz
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Registered: 01/29/09
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296125 - 05/06/09 10:19 AM (14 years, 10 months ago)

Did you inoculate then sterilize :eek:
if so thats bad because thats how you explained it, otherwise put tape and foil over the jars for steam sterilization, if you used a GloveBox and boiled for 90 mins you should be fine, Ive boiled for an hour and been good. Also, its doesnt have to be dark for them to colonize.

Apparantly Incubators arent necessary unless your house temperature is below like 65 but even thats pretty low 70-80 is what I incubate at, so far I havent had any conatms out of 14

good luck


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Offlineblazeup420
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Registered: 04/30/09
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Harlz]
    #10296162 - 05/06/09 10:28 AM (14 years, 10 months ago)

no i inoculated after lol i c wut ur sayin tho i wrote it backwords...wen should i c first signs of myc.?


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:snort: I :oc80mg: LOVE :volcano: MY :bestbud: DRUGS :meff:

                                                        :paranoid:
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OfflineTunesy
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296174 - 05/06/09 10:32 AM (14 years, 10 months ago)

Well recently we had a grow, where there was no sign in 5 days! check the temperature and it was 19C now at 23C and in 2 days lots of growth. So if all is good, in a few days.

Tunes

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OfflineHarlz
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296186 - 05/06/09 10:34 AM (14 years, 10 months ago)

Haha good job :vaped:
id say 5 days is a minimum usually, dont look at them for 2 or 3 days,

I know how hard it is but trust me dude there wont be anything there and you will start stressin

Just be aware of the entire grow, if any wierd smells start happening remove the smelly ones and put them in a seperate location,

dont leave your foil or tape on the holes after your done sterilizing,
This is absolouteley one of the most important things to remember

foil and tape give your mycellium only the amount of oxygen that was in the jar before you taped it, after the mycellium has begun to colonize it will use up all this oxygen and when there is no more oxygen it will stop growing and you will have stalled cakes


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Offlineblazeup420
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Registered: 04/30/09
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Harlz]
    #10296245 - 05/06/09 10:46 AM (14 years, 10 months ago)

nice...damn thats a long fuckin time todays only the second day i just got my mom to give me a thermomiter from her lizards tank so i gotta put that in there the flood lites only on for a couple min at a time though so it should b ok...any fruiting chamber tips(nothing fancy plz)or tips on extra shit to do after colonization?


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:snort: I :oc80mg: LOVE :volcano: MY :bestbud: DRUGS :meff:

                                                        :paranoid:
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OfflineTunesy
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296286 - 05/06/09 10:56 AM (14 years, 10 months ago)

Just read for yourself :smile: its the best thing. Lots of knowledge put together. (Especially RogerRabbit)

Tunes

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InvisibleBeefy1
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Registered: 05/01/09
Posts: 3,573
Loc: around
Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Harlz]
    #10296296 - 05/06/09 10:59 AM (14 years, 10 months ago)

Quote:

Harlz said:

dont leave your foil or tape on the holes after your done sterilizing,
This is absolouteley one of the most important things to remember

foil and tape give your mycellium only the amount of oxygen that was in the jar before you taped it, after the mycellium has begun to colonize it will use up all this oxygen and when there is no more oxygen it will stop growing and you will have stalled cakes




everything I've seen says leave the foil on at least until you inoculate, and the tape should be breathable-let in plenty of air right?

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OfflineSkyHigher
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Beefy1]
    #10296441 - 05/06/09 11:27 AM (14 years, 10 months ago)

I left my jars in the pot I sterilized them in until inoculation. Foil intact. I took them right from the pot to the GB. I used micro pore tape over the lid holes. Injected through the tape. Left tape with needle hole on jar. Discarded Foil. Put in Tit at 80F. Had growth in 2days. Done in about 3 weeks.


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"Total control now. Tooling along the main drag on a Saturday night in Vegas. Two good old boys in a fire-apple red convertible. Stoned. Ripped. Twisted. Good people." - Raoul Duke.

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Offlineblazeup420
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Registered: 04/30/09
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: SkyHigher]
    #10296483 - 05/06/09 11:36 AM (14 years, 10 months ago)

nice hopefully mine colonize  fast


--------------------
:snort: I :oc80mg: LOVE :volcano: MY :bestbud: DRUGS :meff:

                                                        :paranoid:
                          :needdrugs:

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Offlinebrand0n
large jar mad man!!


Registered: 02/09/09
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: blazeup420]
    #10296958 - 05/06/09 01:22 PM (14 years, 10 months ago)

you are looking at this the wrong way right out the gate.

this is a slow process, that takes time, and is a learning curve.

do yourself a huge favor, read this site every day. read the faqs read the posts read read read read read.

also, something i wish someone would have told me

expect failure.

its going to happen, you have to learn from it, but besure to do that..


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That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!

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Offlineblazeup420
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Registered: 04/30/09
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Last seen: 13 years, 7 months
Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: brand0n]
    #10297118 - 05/06/09 02:09 PM (14 years, 10 months ago)

ya i im sure some will b contamed but i did a damn good job being sterile...

About how many dried grams will i get per 1/2 pint cake?


--------------------
:snort: I :oc80mg: LOVE :volcano: MY :bestbud: DRUGS :meff:

                                                        :paranoid:
                          :needdrugs:

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OfflineHarlz
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Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Beefy1]
    #10297141 - 05/06/09 02:15 PM (14 years, 10 months ago)

Quote:

Beefy1 said:
Quote:

Harlz said:

dont leave your foil or tape on the holes after your done sterilizing,
This is absolouteley one of the most important things to remember

foil and tape give your mycellium only the amount of oxygen that was in the jar before you taped it, after the mycellium has begun to colonize it will use up all this oxygen and when there is no more oxygen it will stop growing and you will have stalled cakes




everything I've seen says leave the foil on at least until you inoculate, and the tape should be breathable-let in plenty of air right?




Obviously you leave the foil on until you inoculate and you leave the tape on too, then I remove it in the GB  I assumed he was planning on inoculating immediately after sterilization


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Offlineblazeup420
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Registered: 04/30/09
Posts: 133
Last seen: 13 years, 7 months
Re: couple q's 4 pf tek(firdt time grower)!?!?!?!?!?!? [Re: Harlz]
    #10297214 - 05/06/09 02:33 PM (14 years, 10 months ago)

ya i kept the foil on,let thejars cool then inoculated and the foil is still on gonna take it off wen i get there


--------------------
:snort: I :oc80mg: LOVE :volcano: MY :bestbud: DRUGS :meff:

                                                        :paranoid:
                          :needdrugs:

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