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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
LC question
    #10248844 - 04/28/09 11:20 AM (14 years, 10 months ago)

I have recently been trying to do an LC, but every time it has produced nothing but trich.....and I have been overkilling the sterile procedures.  glovebox, TONS of instant hand sanitizer, scrubbing everything down with iso before using, etc.....anyways, the honey i've been using at 4% mixture seems to not break down entirely when i pressure cook it for an hour....it seems to leave a silty residue on the bottom.  could i be using the wrong type of honey?  if so, does anyone have a recommended brand?


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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

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OfflineChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 11 years, 9 months
Re: LC question [Re: TheMushMan]
    #10248941 - 04/28/09 11:37 AM (14 years, 10 months ago)

You're pressure cooking too long, and the sugar is caramelizing... That's the silt.

Alot of ppl use Karo light corn syrup.


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Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil

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InvisibleDoc_T
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Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: LC question [Re: ChrisWho]
    #10249023 - 04/28/09 11:53 AM (14 years, 10 months ago)

I've had great luck with the honey that comes in a plastic bear.


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You make it all possible. Doesn't it feel good?

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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
Re: LC question [Re: ChrisWho]
    #10249064 - 04/28/09 12:04 PM (14 years, 10 months ago)

Quote:

ChrisWho said:
You're pressure cooking too long, and the sugar is caramelizing... That's the silt.




ah, i see; what's the recommended time that will sterilize it without caramelizing it?


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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

Edited by TheMushMan (04/28/09 12:05 PM)

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Offlineatomic1
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Registered: 09/18/03
Posts: 1,123
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Re: LC question [Re: TheMushMan]
    #10249082 - 04/28/09 12:08 PM (14 years, 10 months ago)

1 tsp. karo/100 mL water
pc @15 psi for 15-20 min.'s
let cool
inoculate
incubate
:mushroom2:

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Offlinebry314159
Stranger
Male


Registered: 04/09/09
Posts: 38
Loc: Midwest
Last seen: 8 years, 10 months
Re: LC question [Re: TheMushMan]
    #10249087 - 04/28/09 12:08 PM (14 years, 10 months ago)

About 15 to 20 minutes.


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"'Tis an ill wind which blows no minds." - Robert Anton Wilson

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InvisibleFractalXplora
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Trusted Cultivator
Re: LC question [Re: TheMushMan]
    #10249088 - 04/28/09 12:08 PM (14 years, 10 months ago)

do a grain LC tek  man, your life will be changed for ever, i never do honey any more. so much more fool proof, search agars posts its in there somewhere.


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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
Re: LC question [Re: atomic1]
    #10249096 - 04/28/09 12:09 PM (14 years, 10 months ago)

Quote:

atomic1 said:
1 tsp. karo/100 mL water





is the process the same with honey?


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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

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Offlineatomic1
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Registered: 09/18/03
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Re: LC question [Re: TheMushMan]
    #10249103 - 04/28/09 12:11 PM (14 years, 10 months ago)

Yup

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OfflineKumara
Dragoness


Registered: 02/24/09
Posts: 179
Last seen: 2 months, 10 days
Re: LC question [Re: atomic1]
    #10249131 - 04/28/09 12:15 PM (14 years, 10 months ago)

It doesn't seem to matter to my myc if there is silt on the bottom, it eats it up and eats everything in the water until it's clear.  I've used honey, and I've used dex/lme with better success, and seen the same thing happen...cloudy to clear.

But, yes, an hour is way too long to pc a liquid culture.

At what point in the process are you seeing contams?

What are you inoculating your LC with?

Are you flame sterilizing the needle if you are using one?

What kind of lids are you using?

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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
Re: LC question [Re: Kumara]
    #10249184 - 04/28/09 12:26 PM (14 years, 10 months ago)

i am seeing the contams after i inject the lc into my wbs jars.
i am inoculating my lc with a biopsy of the inside of the stem.
yes, i flame sterilize (with a blowtorch) and then alcohol wipe it.
i am using self healing rtv injection ported lids.


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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

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Offlineatomic1
enthusiast
Male


Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
Last seen: 4 years, 5 months
Re: LC question [Re: TheMushMan]
    #10249348 - 04/28/09 01:00 PM (14 years, 10 months ago)

Well either your WBS isn't sterilized thoroughly or you're tainting your LC when you add that piece of stem to it and my bet is that latter.  Make sure your tissue is clean.....maybe do a H202 dunk and make the transfer as sterile as possible. Other than that just be sure your WBS is rinsed well b4 and after the soak and thoroughly PC'd. Maybe order up a syringe from a vender and inoculate your LC if you keep having trouble....you can't go wrong that way.

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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
Re: LC question [Re: atomic1]
    #10249481 - 04/28/09 01:28 PM (14 years, 10 months ago)

no, i know it's not my wbs; my spore syringes noc'd em up fine;  and i do an alcohol wipe on the stem before biopsyin' it....is hydrogen pyroxide better to use to sterilize it?


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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

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Offlinemeehi
Being
 User Gallery


Registered: 01/11/05
Posts: 311
Last seen: 3 years, 11 months
Re: LC question [Re: FractalXplora]
    #10249604 - 04/28/09 01:51 PM (14 years, 10 months ago)

Quote:

FractalXplora said:
do a grain LC tek  man, your life will be changed for ever, i never do honey any more. so much more fool proof, search agars posts its in there somewhere.




Here is the link. Pure greatness :thumbup: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0

Although it would not do the cloning that you are wanting to do but seriously give it a read... :yesnod:


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OfflineTheMushMan
Stranger

Registered: 02/06/09
Posts: 291
Last seen: 4 years, 1 month
Re: LC question [Re: meehi]
    #10249750 - 04/28/09 02:16 PM (14 years, 10 months ago)



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Any spore prints I send are intended for microscopic research ONLY.  Cultivation of magic mushrooms is illegal in most countries and nearly every state.  Anything you do with prints from me is your own business and your own responsibility.

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Offlinemeehi
Being
 User Gallery


Registered: 01/11/05
Posts: 311
Last seen: 3 years, 11 months
Re: LC question [Re: TheMushMan]
    #10249867 - 04/28/09 02:40 PM (14 years, 10 months ago)

Super kickass  :cheers:


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