Less than "vigorous" myc.......???? - Mushroom Cultivation

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shroomie_glen
RedHotPussyLiquor

Registered: 03/01/06
Posts: 4,296
Loc: Narf Carolina

Last seen: 11 years, 5 months

Less than "vigorous" myc.......????

#10163173 - 04/14/09 09:52 AM (15 years, 5 days ago)

A pal was showing me recently how he used agar slants from literally 18 months ago to inooculate two dozen or so more slants.
The original slants hadnt been refrigerated, and were dessicated from sitting in room temp for over a year and a half. I mean totally dessicated, 0% moisture.

Sure enough, of 24 new slants transferred to, only 3-5 showed growth worth further transferring. The other 18-20 slants did nothing but get WISPS of mycelial fuzz, no running.

BTW, the original, hella old slants were from clone tissue of an isolate grow.

My question is:

If those viable slants were used to inoculate some grain jars, will there be any noticeable loss in the vigor of the mycelium compared to the original Kick ass speed of the clone tissue used?

Second question:

After colonization, if that grain is spawned to bulk, will more tome need be alotted for colonization/fruiting?

Third question:

Anyone with experience in reviving a seemingly dead tisssue sample, please give me helful tips and pointers on producing fruits from such.

--------------------

No. No, man. Shit, no man. I believe you'd get your ass kicked sayin' somethin' like that man.

Edited by shroomie_glen (04/14/09 09:54 AM)

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shroomie_glen
RedHotPussyLiquor

Registered: 03/01/06
Posts: 4,296
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Last seen: 11 years, 5 months

Re: Less than "vigorous" myc.......????

[Re: shroomie_glen]
#10176425 - 04/16/09 07:54 AM (15 years, 3 days ago)

bizzump shnizzup

--------------------

No. No, man. Shit, no man. I believe you'd get your ass kicked sayin' somethin' like that man.

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German Kahuna
Facepalmer of Stoopid

Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation

Re: Less than "vigorous" myc.......????

[Re: shroomie_glen]
#10176457 - 04/16/09 08:09 AM (15 years, 3 days ago)

I'll answer your third question. I have revived a completely dehydrated piece of an APE's partial veil that was stuck to foil. It is growing. Pretty slow, though. "Vigorous" is one word I'd definitely not use to describe it. I'd say it takes about twice as long as "regular" cubensis mycelium to colonize the grains. Then again, I hear APE is a slow colonizer anyways. I haven't fruited it yet, so I don't know if my trays will bear fruits at all.

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"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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shroomie_glen
RedHotPussyLiquor

Registered: 03/01/06
Posts: 4,296
Loc: Narf Carolina

Last seen: 11 years, 5 months

Re: Less than "vigorous" myc.......????

[Re: German Kahuna]
#10176520 - 04/16/09 08:29 AM (15 years, 3 days ago)

Thats very different, though. There will be spores deposited on the partial veil, which will germinate and produce MS results.

the tissue sample used here, was a clonal piece of internal stem from an already isolated body.

A better question would be:

After total dessication (i mean 100%, NO water left) of a clone tissue sample, when revived, will it have lost its once outstanding vigor due to being "resurrected"?

RR, i know you have a couple pennies to toss at me on this one.

--------------------

No. No, man. Shit, no man. I believe you'd get your ass kicked sayin' somethin' like that man.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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