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Offlinegulthor
Stranger
Registered: 04/06/09
Posts: 30
Last seen: 12 years, 11 months
first time grower needs opinion
    #10158853 - 04/13/09 03:33 PM (14 years, 11 months ago)

Ya i am a first time grower and made a noob mistake.  I had my jars and substrate all steralized in the pressure cooker and was ready to inoculate when i discovered that the syringe was much larger than i anticipated. 

The syringe would not fit through the holes i made.  I now have bigger holes in the lids, but am wondering can i just change the lids and re-steralize in the PC or should i re-mix the substrate. 

The only thing i can think of that may be bad about re-steralizing is that my moisture of the substrate may go down.

What do you guys reccomend i do?

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Offlineveda_sticks
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Re: first time grower needs opinion [Re: gulthor]
    #10158922 - 04/13/09 03:42 PM (14 years, 11 months ago)

You could probably just make up a new lid, then in a glovebox take the old one off, clean the new one very well with alcohol and put it on your jar.

Another option would be to pressure cook your newly mad jar lid in foil, then in the glovebox do a swap.

Be sure to wear gloves, your sheding skin cells all the time.


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OfflineDeadDog
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Registered: 02/18/09
Posts: 498
Loc: La Chorrerra
Last seen: 14 years, 7 months
Re: first time grower needs opinion [Re: veda_sticks]
    #10158976 - 04/13/09 03:50 PM (14 years, 11 months ago)

if you get your pc up to temp,but not under pressure, then put lids with larger holes on jars and put them straight into your pc and tighten the lid and pressurize it as quickly as possible. if you do it like that there wont be enough time for moisture in sub to escape before pc gets up to pressure, which will hold the moisture where it is in ur sub. cook for 90mins, allow to cool naturally w/o removing lid.

you may also want to sit your jars in some rather hot water for a minute or two before putting in pc to make sure the glass doesnt break when u deposit in pc.

will take a bit longer but you will be gaurenteed 100% sterility.


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OfflineSapient
Mycology Student
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Registered: 03/17/09
Posts: 127
Loc: Africa
Last seen: 3 years, 1 month
Re: first time grower needs opinion [Re: DeadDog]
    #10159429 - 04/13/09 04:45 PM (14 years, 11 months ago)

If you are paranoid, replace the lids and resterilise, but simply changing the lids in a glovebox is not as dangerous as it sounds.

Assuming you will go with the first method anyway, pressure cooking again shouldnt dry out your grains too much.

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