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OfflineNeuroticTrip
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Registered: 01/05/07
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Autoclavable bags! Sterilizing substrate! G2bags.
    #10146924 - 04/11/09 09:52 AM (14 years, 11 months ago)

Okay, so I've got my grain jars colonizing, and I've put some autoclavable bags on order. I already have an impulse sealer, and intend on using it because the bags are cheap, and I just like the obsessive level of sterility. (Pasteurization may be an effective technique, but I just want easy results right now).

So I'm wondering how I would go about this. I COULD just fill the bags with grain which has been soaked etc. like the jars, then after sterilization, pull them out, mix in colonized grain, shake, impulse seal... then after colonization cut open, add a casing layer, and use the bag as a fruiting chamber.

OR... I've heard a benefit of autoclavable bags is using mixed substrates. So perhaps I could do a whacky coir/coffee/straw combo or something.

One last important question... Instead of inoculating the bags with grain, could I impulse seal before pressure cooking and use a SPORE SYRINGE to inoculate?

Looking forward to some good shiitake.

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Offlineneopet nub
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Registered: 11/29/08
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Last seen: 13 years, 1 month
Re: Autoclavable bags! Sterilizing substrate! G2bags. [Re: NeuroticTrip]
    #10147143 - 04/11/09 10:56 AM (14 years, 11 months ago)

Don't use spores for coir, it won't work as well and would be purely luck if it did.

BTW, don't steralize your coir/coffee mix.  It will kill all of the beneficial bakkkteria


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OfflineDuggstarMDiscord
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Registered: 01/20/09
Posts: 6,273
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Re: Autoclavable bags! Sterilizing substrate! G2bags. [Re: NeuroticTrip]
    #10147969 - 04/11/09 01:32 PM (14 years, 11 months ago)

It is very unlikely that you will be able to get the spores to germinate on coir, that is why we use grains in the first place.

You seem to have a misunderstanding about pasteurization and sterilization.  We pasteurize because it gets rid of the harmful contaminations but leaves behind beneficial micro-organisms which will actually help your mushrooms grow.  It is not a subordinate technique to sterilization.  The only reason that we sterilize grains is because grains are highly nutritious and very susceptible to contaminations which the mycelium could not compete with, so we need to inoculate them in absolutely sterile conditions.

Edited by Duggstar (04/11/09 01:33 PM)

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OfflineDuggstarMDiscord
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Registered: 01/20/09
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Re: Autoclavable bags! Sterilizing substrate! G2bags. [Re: NeuroticTrip]
    #10147997 - 04/11/09 01:40 PM (14 years, 11 months ago)

Quote:

NeuroticTrip said:
One last important question... Instead of inoculating the bags with grain, could I impulse seal before pressure cooking and use a SPORE SYRINGE to inoculate?

Looking forward to some good shiitake.



I'm not very experienced with edibles, but I have heard that Shiitake rarely fruit from spores anyway.  What you need to do is get some culture from a proven fruiting strain and use that to inoculate.

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OfflineNeuroticTrip
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Registered: 01/05/07
Posts: 119
Last seen: 14 years, 8 months
Re: Autoclavable bags! Sterilizing substrate! G2bags. [Re: Duggstar]
    #10148717 - 04/11/09 05:12 PM (14 years, 11 months ago)

Thanks for the help guys. I'm thinking the answer to my question is to obviously use liquid culture with the autoclavable bags.

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OfflineLaden
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Registered: 09/26/08
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Re: Autoclavable bags! Sterilizing substrate! G2bags. [Re: NeuroticTrip]
    #10148784 - 04/11/09 05:29 PM (14 years, 11 months ago)

Uline has some 4mil poly bags that work well also. I bought a bag of 1000 12x16 off of ebay, and havent even put a dent in em in 4 months of continuous use. there like 65 bucks but i got mine for $20 :smile: i dont impuse seal though. I just fold over the top and loosly tape, then pasturize, and after cool i mix my grain spawn and sub in 12qt kitty litter trays. Then, i cover with alum foil to colonize. works nicly!







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"I believe in a long, prolonged derangement of the senses to attain the unknown. Our pale reasoning hides the infinite from us."
jim:mushroom2::crazy::mushroom2:

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