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DirtyNeedle
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Registered: 04/05/09
Posts: 48
Last seen: 13 years, 11 months
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Noob Question
#10119072 - 04/06/09 07:49 PM (15 years, 14 days ago) |
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Ok I think im just impatient but its been eight days sense I injected 10 1/2 pint jars, 5 Hawaiian and 5 b+. I think I see a little colonization on 2 Hawaiian and 1 b+. I know some jars will be faster then other but I would think I should see growth on all jars by now?
They are in a homemade incubator at 84f so that should be just about perfect for them right?
This is my first attempt ever to grow and im stoked about it and just want at least 50% of the jars to fruit if not more
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oh_you_know
lsd <3
Registered: 02/08/09
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Loc: Oh, USA
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turn the incubator off right now!!!!!!!!!!
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oh_you_know
lsd <3
Registered: 02/08/09
Posts: 2,282
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its takes some time to colonize. 3 jars started after 8 days seems pretty lucky to me. im going into my 4th week and they are about 80% done. 84 degress will kill them for sure. you run the risk of contams because they love high temps. the inside of the jar will be more then the outside so figure 5 degress more inside. you want them about 75 or 70 even. i think you can go as low as 60 for a few hours.
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oh_you_know
lsd <3
Registered: 02/08/09
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wait to see what more people say
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OttoGenerated
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Incubate at room temperature.
The mycelium produce heat as they grow, your internal temperature could be as high as 90°F. This could stall your growth or kill your mycelium, and if it doesn't do either of those it's probably going to lead to contamination.
I hope you can save your jars. Good luck!
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DirtyNeedle
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The house is cold cause its winter here in Mi so what temp should I set the incubator to? 70?
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oh_you_know
lsd <3
Registered: 02/08/09
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yes 70
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DirtyNeedle
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so what temp should i fruit them at assuming everything goes good? I read something about incubating at 80 to mid 80s now im freaked out cause picked up a jar and it was really warm and im afraid I effed up everything!
Edited by DirtyNeedle (04/06/09 08:13 PM)
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OttoGenerated
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I'm new, so don't follow my advice as fact, but:
I don't think the temperature drop is as important as the introduction of fresh air exchange as a pinning trigger. In other words, you don't need to cause a temperature drop.
Wait for a more experienced member to confirm/reject my post. (or do a search, lol)
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OttoGenerated
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If you've had your jars in the mid 80s since inoculation, there's a good chance they're dead.
Don't sweat it, my friend made the same mistake his first go round. Take some pics of what you are supposing is growth, and we'll let you know if it's mycelium.
It's possible that what you're seeing is a combination of wishful thinking and clumps of flour.
This is what the tiniest bit of mycelial growth will look like:
If you have this or anything larger, then congratulations! Your jars survived.
This is what I mistook for growth in my friends jar (this is the grow he overheated):
If it looks like this, just keep waiting it out.
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DirtyNeedle
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getting better pics
Edited by DirtyNeedle (04/06/09 08:59 PM)
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OttoGenerated
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Way too small to tell, sorry.
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init
Golden Student
Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 12 years, 8 months
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Cubes can colonize and fruit just fine at room temperature, so don't worry too much about that.
And if your incubator was at a steady 84 like you said, I seriously doubt that would have "killed" your jars. This species is often more resilient than we give it credit for. 84 is definitely a bit too warm for comfort, but if there's no mycelium in the jar yet, how could the mycelium produce heat? You don't want the internal temperature to ever exceed about 86 degrees, from my understanding, and I doubt you did that. Warmer temperatures invite contamination and can stress the mycelium.. but honestly I don't think you even did any damage. Just keep a watchful eye on the jars for contams.
It took some of my BRF jars 2-3 weeks to germinate, so give them some time. I'm sure they'll be just fine. Best of luck to you!
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DirtyNeedle
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Re: Noob Question [Re: init]
#10119522 - 04/06/09 08:49 PM (15 years, 14 days ago) |
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i fixed the pics
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HiddenIdentity
My real name is John Doe
Registered: 04/03/09
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Your jars are probably not dead. It takes about a temp of 106f to kill myc. I'm sure it stalled the growing though. The biggest risk with higher temps is bacterial growth.
Otto is right though, fresh air is the biggest trigger for pinning. I would incubate at 75, this puts the myc at 80 max. When you fruit, you can drop it to 70. Warmer temps do encourage myc growth, but the risk of contams is higher than the reward of slightly faster colonization.
If you see some green spots, loosen the lids slightly, and boil for an hour - 90 mins. Then you can dump them in the trash, and take it out immediately. Better yet, take the trash bag outside, dump the jars, and then take the trash out.
I am also a noob, I'm just repeating what Ive learned here. A good DVD to pick up is "Lets grow mushrooms" search amazon for it. If you follow that DVD to a T, you will have almost guaranteed success!
Good luck, and welcome to the shroomery!
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init
Golden Student
Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 12 years, 8 months
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Still hard to tell from the pics. If your camera has a mode for taking macro pictures, use that, and rest the camera on a flat surface while taking the pic to help get rid of motion blur.
In that first picture, is that some kind of filter on your jar? Do you have a layer of dry vermiculite at the top of your jars? Perhaps you should describe your setup in greater detail..
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DirtyNeedle
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Last seen: 13 years, 11 months
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Re: Noob Question [Re: init]
#10119626 - 04/06/09 09:05 PM (15 years, 14 days ago) |
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Edited by DirtyNeedle (04/06/09 09:09 PM)
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DirtyNeedle
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Last seen: 13 years, 11 months
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Re: Noob Question [Re: init]
#10119684 - 04/06/09 09:15 PM (15 years, 14 days ago) |
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it is foil under the lid, just didnt clean it up. 1/2 pint jars, brf and ver. injected 1cc per jar. 2 five gallon buckets make the incubator, bottom one with water and aquarium heater with other bucket sitting on top of the water with jars in it, towel on jars then the bucket lid
Edited by DirtyNeedle (04/06/09 09:17 PM)
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init
Golden Student
Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 12 years, 8 months
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Take the foil off! Your jars need to be able to breathe. The dry verm layer acts as your contaminant barrier, so you can leave the holes exposed or cover them with some kind of breathable tape if that makes you feel better. Rip that aluminum off though. I bet you'll see growth pretty soon if you do.
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Mykologist
Often too lazy to post...
Registered: 10/06/08
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Hey Dirty Needle... The OPTIMUM temperature for colonization is 78-81 degrees Fahrenheit. Some growers will insist to use only room temp around 70 to resist bacteria and some mold contaminants, but the mucelium will grow just as healthy and much faster at 80 degrees. Don't go much higher or lower than that. PF cakes usually germinate quickly. People I know have only had a few, ever, that never germinated. It may take from three days to two weeks (a foaf waited TWO AND A HALF WEEKS for germination once!! Turned out to be very healthy mycelium). This hobby will teach you patience of all things. Your jars are fine, I've had closely acquainted friends who incubated jars at 84 and had wonderful results. Fast colo, thick, rhizomorphs, don't worry. You're fine. You're jars are FAR from dead. You shouldn't exceed 81 tho, my friend simply couldn't bring temps below 84. it was out of his control. Fruiting is largely triggered by Fresh Air Exchange (FAE) and light. Temps during fruiting should be around 73, and light should be around 13 hours a day. RH 100% is recommended, especially for cakes. Research Roger Rabbit's Shotgun Terrarium. It's great for the low-key small-scale hobbyist. Good luck man! Post results! Myk Just noticed the foil on your jars. Needs to come off. Do you have a filter below it? Search for tyvek or polyfill filter options. The lack of gas exchange could explain why any jars have stalled. Good luck Myk
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Edited by Mykologist (04/06/09 09:22 PM)
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