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Offlinepftek
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Registered: 08/18/08
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Last seen: 13 years, 5 months
Nothing growing on WBS jars - Resterilize or Just reinject?
    #10111518 - 04/05/09 04:53 PM (14 years, 11 months ago)

I dont see any mold or bacteria growing. I think the solution was just bad as all the other jars are doing okay just the ones using a different syringe.

Should I resterilize in a PC or just reinject? I was using multispore now I'd be injecting with LC.

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OfflineT-Rex
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Last seen: 2 months, 6 days
Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
    #10111647 - 04/05/09 05:14 PM (14 years, 11 months ago)

I would start with new substrate. I've always ended up witha contam by resterilizing a jar.

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OfflineNineInchNails
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Registered: 03/01/09
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
    #10111662 - 04/05/09 05:17 PM (14 years, 11 months ago)

How long ago did you inoculate?

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Offlinepftek
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: NineInchNails]
    #10111723 - 04/05/09 05:26 PM (14 years, 11 months ago)

like 2 weeks ago. it still may show something as it has been taking that long for almost all of my jars. But just in case - plus my lc is almost ready.

Edited by pftek (04/05/09 05:27 PM)

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OfflineT-Rex
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
    #10111763 - 04/05/09 05:31 PM (14 years, 11 months ago)

I'd give it another week. 3 weeks is a bit long. What type of grain are you using??

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Offlinepftek
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
    #10111803 - 04/05/09 05:38 PM (14 years, 11 months ago)

WBS quart jars.

my place is 65 degrees. And when I'm home the temps are closer to 70 degrees.

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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
    #10111978 - 04/05/09 06:01 PM (14 years, 11 months ago)

All i work with is wbs and have found out with wbs sterilization is one of utmost important factors. hey at least you have a few other jars.

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OfflineNineInchNails
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
    #10112471 - 04/05/09 07:26 PM (14 years, 11 months ago)

Personally I think prep of the WBS is very important. I did 9 jars on my first run and all but 3 were a miserable failure. Actually even the 3 that showed life were suffering. I think the WBS was way too dry. This is how I prepared the failing jars:

1. Rinse thoroughly and chuck the floaters. Used garden hose and 5 gal bucket.
2. Soaked for 24 hrs.
3. Rinsed thoroughly again.
4. Spread out on a screen to dry in front of a fan, tumbled occasionally until it didn't stick to my hand.

ONLY 3 jars showed a TINY bit of life after 30 days. I ganked the growth and plopped it into some LC tonight. I used a glove box so I hope to preserve this strain. I don't have anything else to show for the spore syringe I used.

My next batch of jars was a 100% success.

1. Rinse thoroughly and chuck the floaters. Used garden hose and 5 gal bucket.
2. Soaked for 24 hrs.
3. Rinsed thoroughly again.
4. I boiled some water, turned OFF the flame, and tossed in the WBS. Stirred occasionally for 10 min. The WBS was saturated nearly to its core.
5. Spread out on a screen to dry in front of a fan, tumbled occasionally until it didn't stick to my hand.

Every time I've done it this way it's worked out perfectly.

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