Posted by SWBZA (10/16/23 04:35 AM)

Hi.

I'm stuck in understanding Liquid Culture and testing on agar. I have an LC that I made from a Golden Teacher clone. I used this to make another LC by drawing up the first LC in a syringe and injecting this into the new LC. I tested the new LC to agar by drawing up a very small amount of liquid in a syringe and squirting it onto agar and shaking the petri dish to distribute it as much as possible over the agar surface. This is the agar test of the second LC:

Where I'm lost in understanding: I though a clone of a mushroom will be dikaryotic as it is from the fruiting body. Yet you can see the mycelium grew in little islands, and more confusing, the islands of mycelium are not growing together. If I put spores on the agar, I'd expect this "island" behaviour because haploid spores will form monokaryotic mycelium islands. I'd then expect "compatible" islands growing together as they form dikaryotic mycelium. I'd then isolate those "grown together" parts to new agar, giving me a good master to take to grain or LC.

So: why is my LC growing in islands and refusing to grow together? How can a clone be monokaryotic?

BTW, the little red thing at the top between two islands is a piece of my self-healing port that the syringe needle removed :-( So much for making my own SHP with heat resistant red silicone.

Thanks in advance for your help in understanding.

Posted by she loracs (10/08/22 02:38 PM)

i heat dried my amanita @ 170 degrees not knowing the melting point of mycelium, ik ibo acid and muscamol is fine but im wondering if my ambrosia i enooculated yesterday will be viable.

Posted by T5W2L6 (08/06/21 03:03 PM)

"Some add a little H₂O₂ (approx 1-3cc) at this point"

You should mention what percentage of H₂O_{2 is used in your case.}

Posted by Nikker (03/15/16 10:07 AM)

Hi! Thanks so much! I just got what i belive fully colonized honey jars... i want to know if gas exchange is needed in the storage stage, i was wondering about keeping the honey spores in srynges to avoid contamination chances?? Thanks again!! Peace!

Posted by gradolabs (04/17/14 07:19 PM)

Thanks very much for the in depth description.

Posted by MushroomWizard420 (04/13/14 01:31 PM)

Thank you soooo much for this post! I kept hearing LC all over the place but I wanted to start with the basics (PF Tek) and work my way up, totally ignoring LC until curiosity finally made me its bitch. I never imagined LC could turn one syringe/print into a theoretically limitless supply of mycelial potential, let alone cut out the germination portion of the process. I feel so smawt.

Posted by SynapseLotus (02/26/14 05:59 PM)

Using non-organic honey will PREVENT growth!
Edited 2/27/2014 10:01 AM

Posted by some_newb (11/21/12 03:32 PM)

Sorry, but 1 tablespoon light malt extract does NOT equal 10.3g, its more like 20g.

Posted by purplepatterson87 (08/14/12 10:41 PM)

If caramelization occurs after 15-20min of PC, then how would PC-ing again if sediments are present after the first run-through be beneficial? It would seem that running it through a second time would cause that to happen.. No?

Posted by BobbiesDukes (03/30/12 10:43 AM)

Thanks for this! Exactly the information I was looking for.

Posted by sfl (02/20/12 02:50 PM)

would malt extract and agar lc work good with panaeolus cyanescens

Posted by Benteen_Bevalier (10/04/11 11:14 PM)

I feel like steam sterilization would be a better alternative, in response to your last point. (not to PC of course)

Posted by drosmoka (03/27/11 10:45 PM)

may sound stupid butt... would putting food coloring into either the liquid culture (or whatever it is you eventually transplant them to) change the color of your mushrooms? even slightly? wanted to try this at one point but never got around to checking into it :p

Posted by virus1824 (01/13/11 09:25 AM)

""stick in a DARK place with a temp of 82-86F optimally"

Thats also outdated. You can store it in a normal room temperature and normal light cycles do NOT slow growth

Posted by Barakanaten (12/12/10 01:10 PM)

This is very helpful info especially the notes at the end,

having said that I think we can all agree now that H2o2 is very toxic to mycelia

and some go even further as to say H2o2 has no place in anything Myco,

the other side of that coin is some say you can make a diluted mixture of water and H2o2 to combat cob web mold,

RR is quoted as saying this but he also warns of its high toxicity to mycelia.

:sunny:

Posted by anonjon (06/04/10 04:16 PM)

Putting h202 in it seems like a very bad idea to me.

If the lc is contaminated, 3-4 cc of h202 isn't going to fix it.
If the lc is not contaminated, why do it all?

Posted by sporked (08/21/08 02:13 PM)

These liquid percentages seem really overloaded. May vary slightly, 10 grams per 250 ml == 40 grams per liter.

at 40 grams per liter, i was getting somethign that looked vaguely like urine, and wasnt growing anything.

I dilute that now . 25cc "LC concentrate" to 975cc de ionized water, and that seems to be workign considerably better.

Posted by penguinscadoo (03/23/07 08:32 PM)

Edited 3/23/2007 8:05 PM