(05/09/15 10:24 PM)
Whoops! The ordering of the comments here is a bit counter-intuitive :V
(05/09/15 10:23 PM)
There are downregulation mechanisms that prevent the production of one of the products, probably by transcription (easy enough to figure out these days with RNAi).
There would obviously be a limit but adding tryptamine increases total alkaloid content (albeit with a major shift to psilocin and not the phosphorylated counterpart). The best precursors to add would be the ones in the chart that are not a ligand for downregulation.
(04/07/15 12:37 AM)
I don't think it reaches the point of saturation =P
S. cervacia uses the shikimic acid pathway as well and is probably the most widely used laboratory fungi for TA cloning.
If you could find the proteins via western blot and isolate them (you can use glyphosate to inhibit this metabolic pathway to isolate the proteins) and have them sequenced you could get primers and do a PCR to get the nuleic acid sequence. There are commercially available kits for TA cloning into S. cervacia so at that point you could potentially have the yeast produce psilocybin for you B)
(10/30/14 02:56 AM)
That being said, do mushrooms continue to produce psilocybin until veils break or does a small mushroom contain all it will ever produce?