Myco-Bags are auto-clavable filter
patch bags that can be bought online at most mycology supply shops.
The ones I use are the Large bags and run me about $0.25 each in a
box of 100.
So lets get started.
You will need some WBS (Wild
In my case, I am using a 40 pound bag.
I am using 40 pounds. If you want to do a small
batch these bags hold between 3 and 4 quarts each. Just measure the
amount you want into a bucket or pot.
I empty the bag into a
large food grade bucket.
and add water
You want enough water to cover the WBS. Some of it will
float, that is ok for now.
Put the lid on the bucket and let it set for at least 12
hours, 24 is better.
after 24 hours the grain has soaked up a bunch of the water.
There is still plenty in the bucket, but the grain has been
Using a hand strainer we scoop it out of the bucket and put
it into a colander in the sink.
Then rinse the grain with clean tap water. You want to rinse
it until you get clear water out the bottom of the colander. This
will remove the starches that have leached out of the grains. Once
this is done we let it set for 10 to 15 min. This will allow the
water to drain from the grain.
Using a scoop or measuring cup we put a little in the bottom
of the bag to support the bag and hold it in the upright position for
We then fill the bag to about 1/2 way to the filter patch.
(3 to 4 quarts/ 2.839 L -- 3.785 L)
Once the bag is full we fold the top under the bag and
set it aside.
continue this process until all bags are full.
Once you have all the bags full you fold the tops over making
sure to not fold past the filter patch. We then tape the tops closed
with surgical tape.
We are now ready to pressure cook the bags and sterilize
them. Loading your PC (Pressure cooker) may be different depending on
the size of your PC and the size of the bags. I am using a 22 quart
PC and large bags.
We start by placing 2 bags in the bottom
of the PC and use 2 jar rings as separators ro make a little space
between the bags.
Once these bags are in place we add the second layer of bags
at a 90 degree angle to the first.
and place rings between then.
Once the PC is full, we add 4 quarts of water. Put a plate on
top of the bags (to make sure they do not swell and plug the pressure
Now we put the lid on, turn on the stove, and pressure cook
them for 2 hours! The timer begins once the PC has reached pressure
and tempter. You will know it is at pressure when the giggler begins
to giggle as the pressure exceeds 15psi.
Once it is done, let
the PC cool down and de-pressurize. Once the PC is at room pressure
you can open it up and begin to remove the bags.
remove the bags one at a time. We remove the tape holding them closed
and unfold the top. Be careful, it is HOT!
Once the flap is open and while the bag is still hot. We seal
them using a vacuum sealer. We are just using the sealing portion of
the unit. There is no need to use the vacuum portion.
Once the bags are sealed we set them to the side to work on
We also put a piece of duct tape across the top of the bag to
make sure that it does not pop open when we mix the contents up.
Once they are all done, we set them some place to cool. It
can take up to 48 hours for the bags to cool. They need to be cool to
I like to put them in a rubber made container to get them out
of the way.
We can use this time to get the inoculant ready to use.
These are hot inoculation syringes, AKA Liquid culture
That is live mycelia and should get things going quickly.
We remove the bags from where they were being stored and put
a piece of surgical tape on the back of the bag across from the
(You can see the tape just at the top of the
Then we mix the contents of the bag up by hand. This breaks
up the WBS and makes it essayer for the mycelia to colonize.
Now we use a little alcohol. I like to keep it in a squirt
bottle for easy dispensing.
We then take a cotton ball with alcohol on it and wipe the
tape on the bag.
Then using our LC syringe we inject through the tape and put
6cc's of culture into the bag.
We then use another piece of surgical tape to cover the hole
we just made with the needle.
Load them back into the storage place and wait for the
mycelia to do it's job.
Once the bags are half colonized you can break it up by hand
and mix up the contents of the bag. This will help it colonize
faster. Before long they are ready to go.