FastFred's Media Cookbook (v0.98)(Copyright 2004) by FastFred
1. The basics...
A. Classic Favorite Recipes
Potato Dextrose Agar (PDA)(FDA M127) Potato Dextrose Yeast (PDY) Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93) Malt Extract Agar with Yeast (2%)(MEAY) Sabouraud's Dextrose Broth and Agar (SabDex or SDA)(FDA M133)
B. Special Blends
"Karo Water" (Corn Syrup Broth) "Dextrose Tek" Liquid Media (Corn Sugar Broth) Honey Water (Mycotopia Honey Tek) Malt-Yeast-Peptone Agar (McKenna's MYP) Malt Yeast Peptone Agar (Stamets MYP) Grey Cardboard (instead of agar) Oatmeal Flake Agar Moonflower's Rice Malt-Alfalfa-Brewer's Yeast Agar MycoPsycho's Liquid Mycelial Culture Broth [Malt base] Ragadinks Liquid Medium Amaranth Soy Agar EntheoGenesis No.442
C. Food Based Recipies and Variations
Corn Meal Agar (CMA) Cornmeal Dextrose Agar Potato Flake Agar Potato Starch Agar Potato Flake Agar with Yeast Potato-Carrot Agar Barley Flour Malt Extract Agar Barley Flour Modified Sabouraud's Oatmeal Agar (OA) Oatmeal Agar A Oatmeal Agar [B] V-8 Oatmeal Agar V8 Medium Bean Agar Faba Bean Dextrose Agar (FDA) Pea Agar Cabbage Agar Dr. Pollock's Modified [Dog Food] Agar
D. Other Media and Alternate Formulations
Potato-Glucose Agar 1 Potato-Peptone Medium Potato-Peptone-Yeast Agar (PPYA) ------ Malt Agar (MA)(FDA M185)[aka 2% MEA] Difco Malt Extract Broth (FDA M94) Malt Extract Agar for Yeasts and Molds (MEAYM)(FDA M182) Malt Extract Peptone Agar Raper & Thom MEA (RTMEA) ISP 2 Medium (Malt, Yeast, Glucose) ------ Yeast Extract Agar (YEA)(FDA M181) Yeast Glucose Agar Glucose and Yeast Extract Agar Glycerin Yeast Agar ------ Manure Tincture Manure Agar ------ Water Agar (aka Starved Agar) Gelatin Agar (GA)(FDA M54) Plate Count Agar (SMA)(aka Standard Methods Agar)(FDA M124) Nutrient Agar (FDA M112) Starch Agar (FDA M143)(Nutrient agar with starch) Starch-Yeast Agar 1/5 Starch-Yeast Agar Long-term Preservation Medium (FDA M85) Peptone Meat Agar (Meat Water)
E. Common Solutions
Gentamicin Sulfate Solution (FDA M57)
For solid media use a 2% agar concentration. For semi-solid media use 0.2-0.5% agar concentration. Feel free to vary the concentration anywhere in between to get your preferred gel strength. I usually use around 1.5%. Measure this by weight, or figure out some sort of conversion if you don't have a scale. As you can see though, even pretty small measurement errors will give you significant variations in gel strength.
Your carbon source (sugars) should be no more than a maximum 4% concentration. Lowering this promotes faster and more rhizomorphic growth as the mycelium will actively be seeking out more nutrients rather than being saturated and unmotivated.
Section A: Basic recipes
Potato Dextrose Agar (PDA)(FDA M127)
200 g Potato infusion [dilute to 1L total, ~4g solids] 20 g Dextrose 20 g Agar 1 L Distilled water (dH2O)
To prepare potato infusion, boil 200 g scrubbed, sliced(unpeeled) potatoes in 1 liter distilled water for 30 min. Filter through cheesecloth, saving effluent, which is potato infusion (or use commercial dehydrated form). Mix in other ingredients and boil to dissolve. [Dilute to obtain 1 L final volume] Autoclave 15 min at 121