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MRCA Tyroler Gluckspilze
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What are some good agar media recipes?

These recipes are designed mainly for agar maintenance of Psilocybe, but also other mushroom species.



These recipes are designed for agar maintenance of Psilocybe , but also other  mushroom species.
All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure.
500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water

Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)

Amaranth Soy Agar
20 grams amaranth flour
20 grams soy flour
9 grams agar agar
500 ml potable or distilled water

EntheoGenesis No.442
10 grams amaranth flour
10 grams brown rice flour
10 grams potato flour
10 grams soy flour
2 grams malted barley
9 grams agar agar
500 ml potable or distilled water

Oatmeal Neopeptone Agar
40 grams oatmeal or oat flour
2 grams neopeptone (optional)
9 grams agar agar
500 ml potable or distilled water

Modified Sabouraud's Medium
25 grams barley flour
5 grams dextrose
2 grams neopeptone (optional)
1 gram yeast extract
9 grams agar agar
500 ml potable or distilled water

Cornmeal Dextrose Agar
25 grams yellow cornmeal
3 grams dextrose
9 grams agar agar
500 ml potable or distilled water

Barley Malt Extract Agar
40 grams barley flour
2 grams malt extract
1 gram yeast extract (optional)
9 grams agar agar
500 ml potable or distilled water

Dr. Pollock's Modified Agar
10 grams dried dog food (ground to flour)
10 grams amaranth flour
2 grams dextrose or malt extract
9 grams agar agar
500 ml potable or distilled water

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