Home | Mushroom Info | Growing Mushrooms | Archive | General Cultivation | Sterilization and Pasteurization | Tyndallization

Kratom Eye
Please support our sponsors.


How to Sterilize without a pressure cooker

If you dont have a pressure cooker (PC), or cant fit your substrate jars inside your PC (thats me), you can use a sterilization method called tyndallization.  Its a little faffy/time consuming but really keeps those contams at bay.

Tyndallization involves steaming your jars for 20mins, three times, on three consecutive days.  In-between  steaming, let the jars rest at room temp.

Basically some bacterial spores can survive high temperatures, but the heat shock will make them germinate - now they're vulnerable to the next steaming session.

Even late germinating spores will be killed-off during the third steam, leaving your substrate contam free : )

I like to steam for 45mins on the third occation just to be sure!

Happy growing

Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.011 seconds spending 0.001 seconds on 2 queries.