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 LokelYokel
I Gro Fuud
 
Registered: 08/24/08
Posts: 382
Loc: Deep in the Heart
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Quote:
First of all Thank you! If i don't fail i expect to see your left nut in my mailbox..
I'm sure you would put it right next to the 2 that are bumping against your chin.
Look Sporto, you obviously are way too smart to take any advice from anyone here so just go do your thing. If you have spent as much time reading as you say you have, you will have no problems determining the equipment and supplies you need to get yourself up and running. Have fun with your "Rocket Surgery", genius!
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ReUse! RePurpose! ReCycle! "Mom says use it again!"
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 GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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 Re: Shitake Start Up! (help apreciated!)  [Re: ABC]
#10347791 - 05/16/09 10:40 AM (4 years, 9 days ago) |
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I thought i would use the agar to preserve some of the culture for later. I will need agar eventually so might as well order it now. Can bran be substituted with BRF ? I mean in the woodchip sawdust mix ? Havent found bran yet, probably will in a healthfood shop somewhere. Maybe it's a good idea to contact flour mills later. They should have piles of that stuff..
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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Thinking about it now i might actually go with a culture slant. Then again i want to go with Alohas strain ASAP. So don't know..maybe a spore syringe will be enough to get me throgh my first trials. I will be ordering from http://www.mrcashop.org does anyone know about their shiitake strain ? http://www.mrcashop.org/mushroom_shop/lentinula-edodes-shiitake-p-447.html ? Costs as much as the aloha strain. So yeah..im leaning towards the syringe right now.
Edited by GourmetShiitake (05/16/09 10:57 AM)
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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 amusing thread has developed.
I wouldn't go with those guys...why? Because their extremely expensive for no good reason. Go with sporeworks, you can get a grab bag of 4 cultures for $60 (30 pounds) and if you screw up, no biggy. Sporeworks has fantastic strains as it is, they may not be commercial grade but their damn good for those of us playing with Mycology. As well, you'll be able to participate in the free-wedge thread to aquire more cultures to play with. Then once you know you won't lose a culture and you know what your doing, non problem, pay the big bucks for the good stuff.
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Roger Fudd
masshuru-mu uekiya
Registered: 01/27/09
Posts: 420
Loc: Earth, Inner Solar System
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88 euros is quite a lot. I, again, would recommend buying plug spawn. They're cheap...100 for $12. That way, if you screw up on your agar, like everyone does, you won't lose a expensive culture. Using plug spawn is like training. You get to learn the ropes and get some mushies at the end  . If you perform flawlessly with the plug spawn, they you can buy the more expensive cultures. Sporeworks, as Meph said, has great cultures too.
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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Re: Shitake Start Up! (help apreciated!) [Re: Roger Fudd]
#10347852 - 05/16/09 11:09 AM (4 years, 9 days ago) |
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Ya...agar sucks when you start. Pour it wrong...too much sediment...cool the PC too fast, PC is full of agar *cue cursing*. Didn't flame the blade right, contam, bumped the lid against the agar, contam, go to drop agar wedge into grain, falls on table, contam.
 lots of things to totally bomb in. Everyone starting has their plug/LC syringes on backup while having 3-4+ plates as "oh shit" to make sure its not lost. I still have plates that are contammed that I just haven't cleaned yet because I don't want to lose it...but....don't have time yet.
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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Re: Shitake Start Up! (help apreciated!) [Re: Roger Fudd]
#10347871 - 05/16/09 11:19 AM (4 years, 9 days ago) |
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Thanks for the advice guys. Yeah screw 88€..might as well go with aloha. Roger Fudd know any good places to get plug spawn from ? Im ready to try all options. Watching RR-s video right now. Strain isolation is the most interesting section yet. Probably RR-s favorite passtime too..
Oh another thing. Do you guys know a good place for rubber injection ports ?
Thanks guys..
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ABC
Stranger
Registered: 11/16/08
Posts: 1,435
Last seen: 1 year, 5 months
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You'll do fine with sporeworks. They have very good cultures/genetics
BRF contains the bran and germ I think. it's almost the same thing
Also, you won't really need agar at the moment. if you fruit successfully, you will have fruits to clone later.
and even so, you can use cardboard:
http://www.shroomery.org/8512/Cardboard-Cloning-Tek
it is highly recommended that you start from a culture syringe and not spores
self healing injection ports can be made with red RTV silicone, which can be found in automotive shops
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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Oh i see sporeworks actually has a culture syringe not a spore syringe. Sweet..how are those done ? Mycelium in water or smth ?
Edited by GourmetShiitake (05/16/09 11:33 AM)
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Roger Fudd
masshuru-mu uekiya
Registered: 01/27/09
Posts: 420
Loc: Earth, Inner Solar System
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The culture syringes are usually a nutrient water that the mycelium grows in. I would recommend sporeworks or Fungi Perfecti for plug spawn.
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Horticulture
Mycocurious
Registered: 04/30/09
Posts: 1,006
Loc: Midwest
Last seen: 2 months, 3 hours
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Re: Shitake Start Up! (help apreciated!) [Re: Roger Fudd]
#10348351 - 05/16/09 02:19 PM (4 years, 9 days ago) |
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Fungi Perfecti has really solid plug spawn. I've used theirs with good results. You'll probably get from 10-20 more than you pay for, so that's always nice too.
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cyb3rtr0n
searching for truth
Registered: 05/06/09
Posts: 1,787
Last seen: 7 months, 4 days
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Re: Shitake Start Up! (help apreciated!) [Re: ABC]
#10357274 - 05/18/09 08:11 AM (4 years, 7 days ago) |
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wow.... I come to edibles to try and get an intro on how they grow, and this is the first thread I chose to read..
I'm staying inside today, It's already started out with bad luck..
 I'm inoculating this thread with some love.. 
edit:
Oh wait, I didn't realise there was a second page.. it's much better than the first. I think it's safe for me to go outside.
Edited by cyb3rtr0n (05/18/09 08:13 AM)
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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Re: Shitake Start Up! (help apreciated!) [Re: cyb3rtr0n]
#10368395 - 05/20/09 08:38 AM (4 years, 5 days ago) |
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Well i placed an order for plug spawn to fungi perfecti. No feedback yet they didnt have a shipping option for my country. I hope it's not too much. LOL the shipping for a sporeworks culture syringe came out 28$ for an 18$ syringe. So i don't know. BUT i noticed http://www.mrcashop.org also has a plug spawn option. So im probably going to go with that. Atleast it's not spores..
So how should i proceed with the dowels once i get them ? Inoculate the grain spawn with them OR go directly with the wood ? Maybe scrape some mycelium off and inoculate a few agar plates ? What have you done ?
Edited by GourmetShiitake (05/20/09 08:46 AM)
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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Do all of the above. With 100 dowels or so...you got lots of room to play. Try some grain jars, throw some on agar (so you have backup and you can have agar experience) then when you have a backup of the culture that you can keep close (several plates) then you can stick some of them into a log and see what happens. As long as you got a backup, you can make more dowels, grain and more agar plates of it.
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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Awesome..
Just placed my orders should be here in a week. Can't wait. Tomorrow im going to get the pressure cooker, hopefully the bran or BRF as a substitute, the gypsym and im also gonna take a look at the greenhouses..
Fuck i just hope the pressure cooker is the right size. What size pressure cooker is RR using in his woodchip sawdust preparation video? Should really get the All American 41qt ASAP..I visit the states a few times every year so next time hopefully i'll get a few of those..
Edited by GourmetShiitake (05/20/09 10:53 AM)
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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Well mine is a AA 921 and I fit 4 bags @ 6 quarts each (or 6 pounds) nice and snug. I just did some grain with 6 quarts per bag. I think a 941 will fit 6 bags...though don't quote me on it.
And you won't need bran if your using a quart of grain per bag. The bran is a supplement if your using sawdust spawn (which is good for commercial operations looking to save $$ on grains), but if your using a quart of grain per bag, that is your supplement and nutrients right there.
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GourmetShiitake
Stranger
Registered: 03/15/09
Posts: 108
Last seen: 2 months, 23 days
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About the grain..I see RR is using organic rye berries. Can it be substituted with something else as well ? I will probably find rye seeds eventually but right now it would be better if i could just pick something up from the local supermarket or organic food store. I dont want to bother mills for a few kilos of grains i need at this stage.
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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WBS (wild bird seed), red spring wheat, oats, rye berries (organic or otherwise), millet, any cereal grain. WBS is the easiet and quickest if your in a pinch, works well. Don't do rye grass seed...that's a totally different bag of apples and not newb friendly at all. Stick to your grains.
Mills will have 1 bushel bags (55'ish lb bags of grain) so if anything they'd have those, outside that is truckloads.
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Roger Fudd
masshuru-mu uekiya
Registered: 01/27/09
Posts: 420
Loc: Earth, Inner Solar System
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Quote:
Mephistophelian said:
WBS (wild bird seed), red spring wheat, oats, rye berries (organic or otherwise), millet, any cereal grain. WBS is the easiet and quickest if your in a pinch, works well. Don't do rye grass seed...that's a totally different bag of apples and not newb friendly at all. Stick to your grains.
Mills will have 1 bushel bags (55'ish lb bags of grain) so if anything they'd have those, outside that is truckloads.
You forgot corn 
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Mephistophelian
Quasi Hob-Nobbery
Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake
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Re: Shitake Start Up! (help apreciated!) [Re: Roger Fudd]
#10372643 - 05/20/09 11:54 PM (4 years, 5 days ago) |
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Gah! Never...ever Corn. I'm anti-corn (if its spawn that is).
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