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 Psilodelic
Future Mycologist
Registered: 11/18/08
Posts: 17
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 First try Using Grow bag 
#9288415 - 11/21/08 11:28 PM (4 years, 5 months ago) |
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This is the first time ive ever tried to grow mushrooms so im looking for a little advice.
Here is my procedure-
-First i am going to sterilize my work area with disinfectant and wipe everything down with 91% isopropyl alcohol
-Next i will uncap my syringe wipe it with a alcohol soaked guaze pad and heat with a lighter and then wipe again.
-Then i will inoculate the bag by puting about 2-3 ccs of the spore solution into the middle of the substrate. Since i stupidly bout a bag without a self injector site i will then place scotch tape over the hole and duct tape over that.
Then i will shake the **** out of it and put it in a box and put in a dark area that is approximately 75-80 degrees. Then i will wait
Does this sound good?
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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Boris
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Re: First try Using Grow bag [Re: Psilodelic]
#9288442 - 11/21/08 11:33 PM (4 years, 5 months ago) |
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Contrary to popular belief, You don't need to keep them in the dark while incubating. 
Try knocking it up in a glove box, or a very small room with no wind drafts. When you enter the room, open the door slowly to not create air gusts.
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AMU 
Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."
Edited by Boris (11/21/08 11:35 PM)
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Achilles89
Permanent Stranger
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Re: First try Using Grow bag [Re: Psilodelic]
#9288471 - 11/21/08 11:38 PM (4 years, 5 months ago) |
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don't shake it at all. It is important for the spores to be in close proximity for germination. you can shake it once it is about 30% colonized.
Your incubation temps are a bit high too, the mycelium metabolism creates heat, so just shoot for low 70s.
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 J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: Psilodelic]
#9288498 - 11/21/08 11:43 PM (4 years, 5 months ago) |
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Quote:
Psilodelic said:
-Then i will inoculate the bag by puting about 2-3 ccs of the spore solution into the middle of the substrate.
NO 
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Psilodelic
Future Mycologist
Registered: 11/18/08
Posts: 17
Last seen: 4 years, 5 months
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Re: First try Using Grow bag [Re: J3illy]
#9288532 - 11/21/08 11:48 PM (4 years, 5 months ago) |
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No? How should I inoculate then?
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: Psilodelic]
#9288612 - 11/21/08 11:58 PM (4 years, 5 months ago) |
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you wanna squirt the liquid in between the substrate + plastic. you'll see germination as soon as it occurs. injecting in the middle it'll take wks to see anything, and they could be good as gone. with my methods i always see growth in 3-4 days, while i've read so many posts w/ ppl waiting wks. look at this post
http://www.shroomery.org/forums/showflat.php/Number/9282890#Post9282890
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Boris
Viking Warrior, of Valour
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Re: First try Using Grow bag [Re: Psilodelic]
#9288622 - 11/21/08 11:59 PM (4 years, 5 months ago) |
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Not in the middle, let the solution run down the inside side of the bag. I've never did a bag before, but with grains, I always shake the hell out of it after I inocc, and I hear of ALLOT of people shaking bags up after innoccing... The monokaryotic mycelium will germinate with others just the same if they were close. Mycelium generate heat. So whatever temp you keep them at, tack on 10 more degrees and thats what there actually at. comfortable room temp for humans is fine, something about 70-75...
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AMU
Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: Boris]
#9288668 - 11/22/08 12:06 AM (4 years, 5 months ago) |
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i wouldn't shake/mix after knocking a bag. you want those spores close together so they can germinate. only mix the bag once at 30-50%. definitely do not mix before then, or you will be slowing things down drastically, or even preventing growth. when you mix, it takes time/energy for the myc to recover.
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Psilodelic
Future Mycologist
Registered: 11/18/08
Posts: 17
Last seen: 4 years, 5 months
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Re: First try Using Grow bag [Re: J3illy]
#9288671 - 11/22/08 12:07 AM (4 years, 5 months ago) |
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Do you use more than one injection site? Im thinking i will inject through one hole aiming to one side and moving the needle to the other side and inject some without removing it. Should i Inject above substrate and squirt down all over the place or inject right in front of substrate
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: J3illy]
#9288718 - 11/22/08 12:14 AM (4 years, 5 months ago) |
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put the needle in once, and move it around to each inj. site. more sites = better. look at my pics in that link.
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P.Menace
Contradicting the Status Quo
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Re: First try Using Grow bag [Re: J3illy]
#9288795 - 11/22/08 12:26 AM (4 years, 5 months ago) |
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I taught J3 well....
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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J3illy
Trainee
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Posts: 3,344
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Re: First try Using Grow bag [Re: P.Menace]
#9288849 - 11/22/08 12:33 AM (4 years, 5 months ago) |
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skiihigh
underground
Registered: 01/02/08
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Re: First try Using Grow bag [Re: Boris]
#9288859 - 11/22/08 12:34 AM (4 years, 5 months ago) |
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Quote:
Boris said:
Not in the middle, let the solution run down the inside side of the bag. I've never did a bag before, but with grains, I always shake the hell out of it after I inocc, and I hear of ALLOT of people shaking bags up after innoccing... The monokaryotic mycelium will germinate with others just the same if they were close. Mycelium generate heat. So whatever temp you keep them at, tack on 10 more degrees and thats what there actually at. comfortable room temp for humans is fine, something about 70-75...
does it work the same for fruiting? if the FC is 70 will it be 80 after you put the substrate in?
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When the power of love overcomes the love of power, the world will know peace.
-Jimi Hendrix
ALL OF MY POSTS ARE FICTIONAL
Most Outrageous Thread Ive Ever Hijacked---http://www.shroomery.org/forums/showflat.php/Cat/0/Number/9613658/an/0/page/0
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Hieroglyph
Stranger
Registered: 11/21/08
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Re: First try Using Grow bag [Re: Psilodelic]
#9288889 - 11/22/08 12:39 AM (4 years, 5 months ago) |
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umm.. nah don't shake it right after inoculating wait for the mycelium to grow to I don't know maybe 30%? then again at 60-70%?
just don't mess with the bags too much, let them go.
from themadseason:
Locate the small black foam disk near the base of the bag. This disc is the self-healing injector site. Note: The disc will be covered by a small sheet of plastic that attaches it to the rest of the bag. DO NOT remove this sheet, you will inject through it.
Use either a paper towel or cotton swab soaked with rubbing alcohol to wipe the area of the self-healing injection site. This sterilizes the injection area.
Now remove the cap from your spore syringe needle and wipe it down with another alcohol soaked paper towel/swab.
Inoculate the substrate (material inside the bag) with the spore or liquid culture syringe by inserting the needle through the self- healing injection site. Squirt between 2 to 5cc's of spore solution directly into the substrate material. We do not recommend using more than 5cc's of solution or you run the risk of throwing the water content off inside of the bags which could lead to contamination risks later on.
***DO NOT insert the needle through the white filter patch found near the top of the bag.***
Place the bag(s) in a dark location and incubate at the recommended temperatures for the type of mushrooms you are growing. The bag should begin to show signs of growth within 10 days and be completely colonized within 4 weeks or less if the growing parameters are correct.
When colonization is 90-100% complete (bag will be completely white with healthy mycelium), it is time to expose the bags to either indirect sunlight or some kind of artificial light (low-heat florescent light would be best). You also want to lower the temperature down to the proper fruiting temperature of your mushrooms.
After a week or so, pinning should start to occur. The pins should grow into full grown mushrooms within a week.
They should be picked when the caps have just started to open. Waiting until the caps are fully open will cause spores to drop and make a mess in your bag. Cut open the top of the bag with scissors when you are ready to pick them. Carefully pull the colonized substrate out of the bag and pick ALL of the mushrooms and pins completely off of the substrate. When picking is complete, place the block of colonized substrate back into the bag and fold the top of the bag over and close with a clothespin or paper clip.
** Tip - To maximize subsequent flushes, you may dunk the substrate block in distilled water overnight, then dab the excess water off with a paper towel and place back into the bag. To close the bag, just fold the top over and use either a clothespin or paper clip to hold the fold in place.
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Psilodelic
Future Mycologist
Registered: 11/18/08
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Re: First try Using Grow bag [Re: Hieroglyph]
#9289076 - 11/22/08 01:12 AM (4 years, 5 months ago) |
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Well I just finished innoculating my bag. I wiped everything down, wore gloves and covered them in alcohol, flamed and wiped the needle. I inoculated in 4 different spots. I think i did everything right but i did forget to wipe the injection spot before innoculating i hope it still grows alright.
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
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Re: First try Using Grow bag [Re: Psilodelic]
#9289085 - 11/22/08 01:14 AM (4 years, 5 months ago) |
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Quote:
Psilodelic said:
Well I just finished innoculating my bag. I wiped everything down, wore gloves and covered them in alcohol, flamed and wiped the needle. I inoculated in 4 different spots. I think i did everything right but i did forget to wipe the injection spot before innoculating i hope it still grows alright.
thats contradicting
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: P.Menace]
#9289098 - 11/22/08 01:17 AM (4 years, 5 months ago) |
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I forgot to wipe the port on 1 of my bags and everything is going good so far w/ healthy growth.
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Psilodelic
Future Mycologist
Registered: 11/18/08
Posts: 17
Last seen: 4 years, 5 months
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Re: First try Using Grow bag [Re: P.Menace]
#9289145 - 11/22/08 01:24 AM (4 years, 5 months ago) |
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Quote:
Psilodelic said:
Well I just finished innoculating my bag. I wiped everything down, wore gloves and covered them in alcohol, flamed and wiped the needle. I inoculated in 4 different spots. I think i did everything right but i did forget to wipe the injection spot before innoculating i hope it still grows alright.
I made one injection site and move to four different spots
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: Psilodelic]
#9289209 - 11/22/08 01:36 AM (4 years, 5 months ago) |
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Are you doing only 1 bag? That's kinda having all your eggs in 1 basket. Reason I say this is cuz I ordered 5 and started w/ 2, 1 Ecuador and 1 Amazon - I ended up finding trich in the Ecuador on Day 11. I will say tho, I had a feeling something was up, cuz the myc. was growing really slow in that 1, compared to the Amaozn, but I thought maybe Ecuador was just a slow colonizer.
Well the next 3 bags were 2 Amazon and 1 Ecuador, and I was just expecting the Ecuador bag to get trich again [I inoculated it AFTER I found the trich in the other 1], and this Ecuador bag is doing great - growing just as fast as the Amazons, in fact 1 of the Amazons is actually the slowest.
What I'm getting at is, even tho these bags are easy, you can still end up getting a contam or messing up. And it would really suck to have it be your only bag. I was VERY good w/ my inoculation - Oust, gloves, alc. wipes, etc.. No glove box tho, and a bag still got trich. Good luck
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Psilodelic
Future Mycologist
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Re: First try Using Grow bag [Re: J3illy]
#9289281 - 11/22/08 01:50 AM (4 years, 5 months ago) |
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Yea i only bought one bag cause i just wanted to try it out but i am sterilizing a lot of wbs jars tommorow so i can try with a bunch because i figure i will mess a few up at least. But i hope the bag works too lol.
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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shroomophile
ShroomitusFidelis
Registered: 08/20/02
Posts: 762
Loc: USA
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Re: First try Using Grow bag [Re: Psilodelic]
#9289316 - 11/22/08 01:54 AM (4 years, 5 months ago) |
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Seems like a lot of trouble for 1 bag.PM your addy and i will send you ten bags.
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Once the mighty oak,was a nut who held his ground.
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Psilodelic
Future Mycologist
Registered: 11/18/08
Posts: 17
Last seen: 4 years, 5 months
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Re: First try Using Grow bag [Re: shroomophile]
#9289410 - 11/22/08 02:16 AM (4 years, 5 months ago) |
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How do i PM the addy
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You can turn your back on a person, but, never turn your back on a drug-Raoul Duke
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: Psilodelic]
#9289418 - 11/22/08 02:18 AM (4 years, 5 months ago) |
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10 bags? I bet he's talking about empty ones that you'd have to fill/sterilize yourself.
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P.Menace
Contradicting the Status Quo
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Re: First try Using Grow bag [Re: J3illy]
#9289471 - 11/22/08 02:27 AM (4 years, 5 months ago) |
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10 free bags is worth near 20 free dollars
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
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Re: First try Using Grow bag [Re: P.Menace]
#9289512 - 11/22/08 02:34 AM (4 years, 5 months ago) |
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shit id like a few of those bags too
-noobie-
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AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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shroomophile
ShroomitusFidelis
Registered: 08/20/02
Posts: 762
Loc: USA
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Re: First try Using Grow bag [Re: J3illy]
#9289538 - 11/22/08 02:38 AM (4 years, 5 months ago) |
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Yeah they are empty,but 10 bags+i bag makes 11 bags.Elementary algebra.
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Once the mighty oak,was a nut who held his ground.
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shroomophile
ShroomitusFidelis
Registered: 08/20/02
Posts: 762
Loc: USA
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Re: First try Using Grow bag [Re: Psilodelic]
#9289547 - 11/22/08 02:40 AM (4 years, 5 months ago) |
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Click my user name and then look at the bottom and you'll see send a PM.
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Once the mighty oak,was a nut who held his ground.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: shroomophile]
#9289661 - 11/22/08 03:02 AM (4 years, 5 months ago) |
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Quote:
shroomophile said:
10 bags+i bag makes 11 bags.Elementary algebra.
Basic arithmetic, ya mean? 
It's definitely generous of you, but his bag is a pre-made bag. The reason why ppl even get them is usually so they don't have to deal w/ making a substrate, sterilizing it, hoping it's good to go, etc.. It's definitely cheaper to make your own, but just saying.
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shroomophile
ShroomitusFidelis
Registered: 08/20/02
Posts: 762
Loc: USA
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Re: First try Using Grow bag [Re: J3illy]
#9289999 - 11/22/08 04:15 AM (4 years, 5 months ago) |
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10 + X =11. Algebra.
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Once the mighty oak,was a nut who held his ground.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: First try Using Grow bag [Re: shroomophile]
#9290015 - 11/22/08 04:19 AM (4 years, 5 months ago) |
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That's just making it more complicated than it has to be. The amount of bags he has now is a constant at 1, so why would we assign a variable to it? 
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JazzCatCF
Teo-nanacatl
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Re: First try Using Grow bag [Re: Psilodelic]
#9290533 - 11/22/08 10:08 AM (4 years, 5 months ago) |
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Ive noticed growth speeds way up when held at 73 degrees.I started my bags at 78 then started 4 more bags and left them at 73 and the 73 degree bags took about 2 weeks to colonize and the 78 took alittle over 3 weeks.Also I prefer to use colonized grain vs lc straight in the bag.for me its way faster.I think bags are the way to go,although I get better yeilds with casing/greenhouse.I would say just make up about 20 bags and experiment different ways with innoc,colonization and fruiting and find whats best for you.
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"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."
The Mazatec Wise Woman, Maria Sabina (1894-1985)
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