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OfflineCapnSkoo
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WBS TEK Questions
    #8570919 - 06/27/08 02:42 PM (4 years, 11 months ago)

A few WBS TEKs Ive read state to use polyfill/tyvek/coffee filter combo for contam control. Could i just inoculate in a  glovebox and top the holes with the micropore tape I already have? Would this be sufficient enough to keep the nasties out? Thanks Guys.


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:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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Offlinehxcjohn
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Re: WBS TEK Questions [Re: CapnSkoo]
    #8570932 - 06/27/08 02:45 PM (4 years, 11 months ago)

I don't see why not.


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OfflineJMR
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Re: WBS TEK Questions [Re: hxcjohn]
    #8570950 - 06/27/08 02:52 PM (4 years, 11 months ago)

yes it will


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: JMR]
    #8571291 - 06/27/08 04:41 PM (4 years, 11 months ago)

I thought it would but "better to ask than look like an ass."


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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Offlinehxcjohn
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Re: WBS TEK Questions [Re: CapnSkoo]
    #8572195 - 06/27/08 09:29 PM (4 years, 11 months ago)

I thought it was better to search than look like an ass :wink:


Edited by hxcjohn (06/27/08 09:30 PM)


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: hxcjohn]
    #8572503 - 06/27/08 11:06 PM (4 years, 11 months ago)

Whats wrong with doing both?


--------------------
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"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: CapnSkoo]
    #8572528 - 06/27/08 11:15 PM (4 years, 11 months ago)

Plus, I'm a hands on visual learner so all this reading of conflicting and dated TEKs is causing my brain to overheat. I learned more watching RR's videos in those few minutes than I ever did spending hours reading TEKs. I'm gonna ask questions because it helps me understand.


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8572566 - 06/27/08 11:25 PM (4 years, 11 months ago)

Micropore works fine, but I recommend using tyvek.  Micropore tends to lift every once in a while due to moisture and what have you.  Tyvek is free at the local post office and is just kick ass!



The Shroomy 1


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: The shroomy 1]
    #8572951 - 06/28/08 01:31 AM (4 years, 11 months ago)

Free huh? Cant beat that with a stick.


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8573033 - 06/28/08 02:11 AM (4 years, 11 months ago)

Yea, they usually have a nice stack of them around the mailing area.  Don't go crazy or anything.  One envelope yields 10 perfect circles, (Five on each side).  I visit my local Post office once a week and take about 5 envelopes.  If your interested search the posts and see what others have to say about it.  I absolutely love it.

The Shroomy 1


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Re: WBS TEK Questions [Re: The shroomy 1]
    #8573257 - 06/28/08 03:44 AM (4 years, 11 months ago)

I personally think doing both tyvek and polyfill is overkill. I just stuff some poly into the wholes. Its really cheap or free and doesn't require cutting and its real minimal.


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: thepaintingaccid]
    #8574291 - 06/28/08 03:13 PM (4 years, 11 months ago)

Well thanks guys. I've got my WBS in a pot soaking to allow the germination of endospores per instructions in Docs WBS TEK. Seems like a good TEK. I've got some BRF jars going but I want at least one flush by the Sturgis Motorcycle Rally which is the first weekend in Aug. Do yall think this is realistic? I've read that WBS half/pints can be ready to case or spawn in as little as 14 days and as long as 28 days. Whatdyall think?


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8574556 - 06/28/08 04:54 PM (4 years, 11 months ago)

yah thats a good timeframe.


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Offlinefiggusfiddus
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Re: WBS TEK Questions [Re: CapnSkoo]
    #8574615 - 06/28/08 05:16 PM (4 years, 11 months ago)

Quote:

CapnSkoo said:
Well thanks guys. I've got my WBS in a pot soaking to allow the germination of endospores per instructions in Docs WBS TEK. Seems like a good TEK. I've got some BRF jars going but I want at least one flush by the Sturgis Motorcycle Rally which is the first weekend in Aug. Do yall think this is realistic? I've read that WBS half/pints can be ready to case or spawn in as little as 14 days and as long as 28 days. Whatdyall think?




You'll get a flush out of the BRF before then if that's what you're asking. With the WBS, it's always hard to say how long it will take you to be ready for spawn, plus how long the spawn run itself will take. But I'd say August is realistic, though optimistic.


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: figgusfiddus]
    #8574710 - 06/28/08 05:43 PM (4 years, 11 months ago)

I started the BRF with multi-spore on the 24th and 25th. Havent noticed any real signs of colonization yet but i expected that. I've read that BRF cakes take longer to colonize than WBS. Plus the jars started on the 25th were a little denser and perhaps wetter. I'm planning this next run on WBS and the one after that, rye berries. Also, i built several RR Shotgun FC's for the cakes. Would it be better to bulk spawn the WBS in HPOO/Straw into Mono's or could i use the shotguns just as well?


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8574726 - 06/28/08 05:48 PM (4 years, 11 months ago)

fruiting from strait wbs is not worth the time. Spawn to bulk but go at a higher ratio 1:4 to speed up colonization. And grains if done right will reach 100% colonization faster than cakes.


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Re: WBS TEK Questions [Re: thepaintingaccid]
    #8574791 - 06/28/08 06:06 PM (4 years, 11 months ago)

BRF takes a little longer to fully colonized in my experience as well, but not by too much time compared to WBS (maybe a week tops?).


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Re: WBS TEK Questions [Re: thepaintingaccid]
    #8576618 - 06/29/08 09:08 AM (4 years, 11 months ago)

Quote:

fruiting from strait wbs is not worth the time.














Those casings were straight WBS cased with coir.

The advantage to casing WBS straight is that you do not have to wait for the substrate to fully colonize. This can/will knock off a substantial amount of time from your grow. About 2 weeks of time.
Using straight WBS if prepared properly(as in Doc's Wild Bird Seed Tek),  inoculated from a LC, straight cased with coir only, can and will give you fruits in less than 21 days!(safely speaking) Or at least that is how it is in Doc's world. Straight casing WBS is the fastest way to fruits plain and simple. The only downside to doing it this way is the size of the fruits. They appear to be quite small on the first flush, but, do get a lot larger with consecutive flushes as in the last 3 pics above, which were on the second flush, compared to the first pics, which were on their first flush.
Other than that, I like to use straight grains when I am in need of shroom fix and need it quick, :grin:

If you use a LC, your grain jars should colonise in 6-10 days(quarts).
If you use a Multi-spore inoculation your grain jars should reach full colonization in about 10-20 days.
If you straight case your grains(as a substrate) you should see fruits in about 14 to 21  days.
If you spawn your grains to a substrate such as straw/poo you should see fruits in about 21-30 days.


My two pennies.


Doc


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Re: WBS TEK Questions [Re: doc34]
    #8577630 - 06/29/08 05:03 PM (4 years, 11 months ago)

well doc I commend you on a spectacular disapproval.:smirk: how many quarts of wbs per box? And isolate or ms?


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: thepaintingaccid]
    #8577823 - 06/29/08 06:21 PM (4 years, 11 months ago)

So I just pulled my WBS 1/2 pints from the PC and it looks like more grain split open. I assume this is okay. I'm gonna inoculate in the morning in my glovebox. Planning on casing 1.5 pints into a 12x7 cakepans with hpoo/straw. These i will place into my shotgun fc's for fruiting. I still see no colonization in my brf jars. But in the eventuality that the WBS and BRF finish colonizing at the same time i'll just leave the BRF cakes in the dunk until the WBS is spent. Any other input guys?


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8577856 - 06/29/08 06:34 PM (4 years, 11 months ago)

Quote:

CapnSkoo said:
So I just pulled my WBS 1/2 pints from the PC and it looks like more grain split open. I assume this is okay. I'm gonna inoculate in the morning in my glovebox. Planning on casing 1.5 pints into a 12x7 cakepans with hpoo/straw. These i will place into my shotgun fc's for fruiting. I still see no colonization in my brf jars. But in the eventuality that the WBS and BRF finish colonizing at the same time i'll just leave the BRF cakes in the dunk until the WBS is spent. Any other input guys?




No need to worry yet on BRF colonization, but just curious as to where you got your spores, and what strain?

The straight WBS substrate pics are very interesting, Doc, thanks!


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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: Mad River]
    #8577873 - 06/29/08 06:43 PM (4 years, 11 months ago)

Got my spores from Hawks Eye. B+ and Golden Teacher Excellent service. And I'm really not expecting any visual signs in the BRF jars for several more days. Soo..... time to wait again. Gonna start some rye berries next weekend. Seems I've got jars and syringes coming out the demonhole. Best use 'em up and stay busy. I suppose in a few weeks I'll be REAL busy.


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8577885 - 06/29/08 06:50 PM (4 years, 11 months ago)

Seeing signs of growth wouldn't be out of the question, but like I said nothing to worry about. Thanks for the feedback on Hawk's Eye. :thumbup:


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Re: WBS TEK Questions [Re: Mad River]
    #8578266 - 06/29/08 08:45 PM (4 years, 11 months ago)

I got my shipment days ahead of schedule and received prompt replies to my emails. Took another look at my jars. The ones knocked on the 25th are starting to take off. Small 1-2mm spots on the side of the jars at the inoculation points. We'll see.


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

-I Need Money


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Re: WBS TEK Questions [Re: CapnSkoo]
    #8579112 - 06/30/08 12:47 AM (4 years, 11 months ago)

Sorry to derail the thread but this goes back to the first questions the OP had.
I've got some WBS jars cooking right now. I punched a single hole in the middle of the lid quart jars metal lid, placed that on the jar along with a single layer of tyvek and secured it down with the metal band. When I go to inoculate the jars, what would be the best means of covering the whole the needle punched through the tyvek?


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Re: WBS TEK Questions [Re: SFsorrow]
    #8579152 - 06/30/08 01:00 AM (4 years, 11 months ago)

Micropore tape.


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Re: WBS TEK Questions [Re: Mad River]
    #8579201 - 06/30/08 01:20 AM (4 years, 11 months ago)

Ok, is it bad that I get the tyvek wet with 70% alcohol before I apply the micropore tape? I've had some issues with contamination before. I'm not sure if it was the LC syringe I made up, the sterilization techniques I used for inoculation or what. See: this thread.
Thanks!


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Re: WBS TEK Questions [Re: SFsorrow]
    #8579300 - 06/30/08 01:59 AM (4 years, 11 months ago)

I've never used tyvek and haven't had problems with contamination. *knock on wood*

But I love using WBS. So efficient, cheap, and quick.


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Re: WBS TEK Questions [Re: SFsorrow]
    #8579857 - 06/30/08 09:43 AM (4 years, 11 months ago)

Quote:

SFsorrow said:
Sorry to derail the thread but this goes back to the first questions the OP had.
I've got some WBS jars cooking right now. I punched a single hole in the middle of the lid quart jars metal lid, placed that on the jar along with a single layer of tyvek and secured it down with the metal band. When I go to inoculate the jars, what would be the best means of covering the whole the needle punched through the tyvek?



make separate silicone self-healing injection points. That way you don't need to touch your tyvek/micropore tape.



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OfflineCapnSkoo
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Re: WBS TEK Questions [Re: roquet]
    #8579911 - 06/30/08 10:21 AM (4 years, 11 months ago)

Knocked up my 6 1/2 pint jars of WBS this morning with B+. I think I'm seeing some colonization in one of my BRF's but it could just be a piece of verm pressed up against the glass. Goin huntin fro hpoo tonight. Its been somewhat dry here lately so I should come home with a fairly significant haul. Also, if I wanted to pasturize in the field how long would this hpoo keep? I'm kinda worried about the smell. I do live in an apartment building. Input?


--------------------
:dancer:Disco! Disco!:dancer:


"Lord its a Gift! Its a Gift from Above! Make it float like a Dove! Make it shout like Mike with a Rhinestone Glove!"
 

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OfflinePsilocelium
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Re: WBS TEK Questions [Re: CapnSkoo]
    #8579966 - 06/30/08 10:54 AM (4 years, 11 months ago)

Instead of Tyvek or Polyfill, I have been using those little circular band-aids. I have never had them fail on me.


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Re: WBS TEK Questions [Re: doc34]
    #9310956 - 11/25/08 03:53 PM (4 years, 6 months ago)

I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.  I am so confused, I filled 2/3 of WBS in 4 qt jars and PC'd the jars for an hour each.  I had low moisture content, and inoculated them in the perfect setting.  I flame sterilized my syringe between each jar
and 1-2 cc's of TEX in each jar.  At least that's what it should have been, is it normal to receive the syringe with the spores already in the syringe ready to inoculate? 

Well anyways it has been 4 days now and I see no signs of anything.  The temp is at 85 degrees and what seems like was a sure thing is turning into a lost cause

any suggestions???


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Re: WBS TEK Questions [Re: cultmember]
    #9311077 - 11/25/08 04:14 PM (4 years, 6 months ago)

Quote:

cultmember said:
I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.  I am so confused, I filled 2/3 of WBS in 4 qt jars and PC'd the jars for an hour each.  I had low moisture content, and inoculated them in the perfect setting.  I flame sterilized my syringe between each jar
and 1-2 cc's of TEX in each jar.  At least that's what it should have been, is it normal to receive the syringe with the spores already in the syringe ready to inoculate? 

Well anyways it has been 4 days now and I see no signs of anything.  The temp is at 85 degrees and what seems like was a sure thing is turning into a lost cause

any suggestions???




I took this from the exact post you made in the other thread.

4 days???? COME ON DUDE!!! hahaha I'm just saving you from the abuse you may get - cuz 4 days is NOTHING.

Also 85 is TOO HIGH and will slow down growth AND promote contams.
Get it at or below 80.  GO for 75 to be safe, cuz you'll notice no diff.

And I really hope you Pressure cooked those WBS.


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Re: WBS TEK Questions [Re: CapnSkoo]
    #9311109 - 11/25/08 04:19 PM (4 years, 6 months ago)

post pics when your done. =]


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Re: WBS TEK Questions [Re: cultmember]
    #9311405 - 11/25/08 05:14 PM (4 years, 6 months ago)

Quote:

cultmember said:
I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.
...
any suggestions???




Dude? I've no idea what went wrong for you. These are my friends jars 20 hours after inoculation:


Oh, I forgot to say that after inocculating the WBS jars, she used the oven tek to incubate them for 6 hours at 350F, since the warmer the medium is, the quicker the spores will germinate and grow. After the 6 hours, she shut the oven off and waited overnight.


I guess keep waiting?

:laugh:


Edited by SFsorrow (11/25/08 05:23 PM)


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InvisibleJ3illy
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Re: WBS TEK Questions [Re: SFsorrow]
    #9311462 - 11/25/08 05:22 PM (4 years, 6 months ago)

:lol:


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Re: WBS TEK Questions [Re: SFsorrow]
    #9311486 - 11/25/08 05:26 PM (4 years, 6 months ago)

Quote:

SFsorrow said:
Quote:

cultmember said:
I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.
...
any suggestions???




Dude? I've no idea what went wrong for you. These are my friends jars 20 hours after inoculation:


Oh, I forgot to say that after inocculating the WBS jars, she used the oven tek to incubate them for 6 hours at 350F, since the warmer the medium is, the quicker the spores will germinate and grow. After the 6 hours, she shut the oven off and waited overnight.


I guess keep waiting?

:laugh:




thats harsh man..

---

i always put my tyvek under the lid, always had success too, hmm.


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Re: WBS TEK Questions [Re: BEEP]
    #9311554 - 11/25/08 05:34 PM (4 years, 6 months ago)

Quote:

JoelKl1 said:
thats harsh man..




I thought it was light hearted given all the info otherwise.

What ended up working for me was making an injection port using RTV gasket sealant in the middle of the lid and a small cluster of wholes on the outer side, covering them with tyvek and securing them down with micropore tape.



Then just flame the needle between each injection, making sure you wipe down the lid with alcohol, and you should be golden, and be patient!
Also a still air box is your best friend.


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OfflineMad River
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Re: WBS TEK Questions [Re: cultmember]
    #9311648 - 11/25/08 05:47 PM (4 years, 6 months ago)

Quote:

cultmember said:
I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.  I am so confused, I filled 2/3 of WBS in 4 qt jars and PC'd the jars for an hour each.  I had low moisture content, and inoculated them in the perfect setting.  I flame sterilized my syringe between each jar
and 1-2 cc's of TEX in each jar.  At least that's what it should have been, is it normal to receive the syringe with the spores already in the syringe ready to inoculate? 

Well anyways it has been 4 days now and I see no signs of anything.  The temp is at 85 degrees and what seems like was a sure thing is turning into a lost cause

any suggestions???




Did you do any soaking of your WBS? You didn't mention that.


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InvisibleThe shroomy 1
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Re: WBS TEK Questions [Re: SFsorrow]
    #9311678 - 11/25/08 05:51 PM (4 years, 6 months ago)

Quote:

SFsorrow said:
Quote:

cultmember said:
I inoculated 4 jars of TEX in WBS four days ago and I have seen no signs of growth.  I have no clue what I did wrong.
...
any suggestions???




Dude? I've no idea what went wrong for you. These are my friends jars 20 hours after inoculation:


Oh, I forgot to say that after inocculating the WBS jars, she used the oven tek to incubate them for 6 hours at 350F, since the warmer the medium is, the quicker the spores will germinate and grow. After the 6 hours, she shut the oven off and waited overnight.


I guess keep waiting?

:laugh:


 

Please don't spread those kind of shinanigans, (for lack of a nicer word), around here.  Someone might take it as if it was the ttruth.

The Shroomy 1


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