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 IFEELGOOD
Stranger
Registered: 09/18/06
Posts: 76
Last seen: 6 years, 1 month
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 Re: LC Cloning - Newbie friendly pictorial tek!  [Re: hucker696]
#6166528 - 10/13/06 06:44 PM (6 years, 7 months ago) |
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haha im a failure this sounded so easy but I stabbed my poor mushroom like 50 times and couldn't get a visible piece in the needle. Maybe for me I should just cut off a few lil pieces and put them in the hole manually ? lol
I'm serious I tried I feel like a failure. Maybe one got enough in it and it will come out Ok...
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 creamcorn
mad scientist
 
Registered: 03/13/06
Posts: 2,962
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: IFEELGOOD]
#6166600 - 10/13/06 07:04 PM (6 years, 7 months ago) |
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A few things that help are a larger gauge needle, stabbing at an angle while you go through, and twist as you stab - the sort of angled "sawed off" part of the syringe will help you cut a nice core. Obviously the thicker/meatier the mushroom, the better. Its not just you, it can be a little tricky at times... so don't feel like a failure, I've screwed up my fair share of these too.  Luckily it takes a few cents and a few minutes to make another LC jar so there's not much at stake.
You absolutely can cut a piece out and start your LC that way... the coring/biopsy technique is probably the most difficult way to get a sample, but the easiest way to transport a sterile sample from mushroom to culture. On the other hand you can cut a piece out easy, but the hard part is getting a sterile piece into a sterile culture since it would involve exposing more mushroom, and opening the lid to your LC container. If you have a glovebox (or even take a few minutes and throw together a ghetto cardboard-box-saran-wrap glovebox) you can definitely pull it off with a scalpel - slice a mushroom open, cut out a chunk, stab it, open LC and quickly transfer inside, and bam. 
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Hotnuts
old hand
Registered: 02/26/05
Posts: 3,434
Loc: Wild Blue Yawnder
Last seen: 11 days, 9 hours
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: IFEELGOOD]
#6166601 - 10/13/06 07:05 PM (6 years, 7 months ago) |
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Are you sure those cultures are clean creamcorn? They appear to be a bit cloudy IMO. Just wondering if you've tried them?
Edited by Hotnuts (10/13/06 07:07 PM)
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: Hotnuts]
#6166638 - 10/13/06 07:13 PM (6 years, 7 months ago) |
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The cultures shown worked great. They're always under refrigeration so when I took them out to take snapshots, the cold made water condense on the outside of the jars... could be what you're seeing? Or maybe a combination of camera tricks and thick as hell LC.
In fact, the "dimple cap" substrain I was just going off about on in that other thread about the strains (and in my avatar picture) is what you see in those two jars of colonized LC.
I actually contaminated my master culture (the half pint) of that after the fact.  I've still got that quart jar that was pictured on the mag stirrer in my fridge since July... little less than a half of it left... just 'nocced some last week and those WBS jars are almost colonized, so its still hanging in there.
Coincidentally, the one I prepared while taking the pictures never even actually worked... I was a bit less than sterile because I was trying to photograph things.
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IFEELGOOD
Stranger
Registered: 09/18/06
Posts: 76
Last seen: 6 years, 1 month
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#6169129 - 10/14/06 04:11 PM (6 years, 7 months ago) |
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thanks cream i'll probably have to give it another try.
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BallsDeep
Misanthropist
Registered: 07/31/06
Posts: 270
Last seen: 6 years, 20 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: IFEELGOOD]
#6169221 - 10/14/06 04:52 PM (6 years, 7 months ago) |
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What happens if you clone an abort??
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My judgment day is everyday
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coastalite
Stranger
Registered: 01/13/07
Posts: 1
Last seen: 6 years, 4 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#6462345 - 01/15/07 04:11 PM (6 years, 4 months ago) |
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Quote:
creamcorn said:
"Its important to take from the master each time, rather than go from working culture to working culture, so that each of your working cultures are "second generation"."
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OK, why do you want to use a "second generation" liquid culture to inoculate your jars? Can't you just use your master to inoculate your jars and then when you get low you just make another master? Or is this done only to lessen the risk of contaming the master?
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Essobee
Stranger
Registered: 12/04/06
Posts: 11
Last seen: 6 years, 1 month
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: coastalite]
#6462518 - 01/15/07 04:52 PM (6 years, 4 months ago) |
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Excellent stuff. Thanks Creamcorn.
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fetalscab
Registered: 12/28/06
Posts: 460
Loc: not quite sure? 
Last seen: 4 months, 12 days
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 Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#6501036 - 01/27/07 01:54 AM (6 years, 3 months ago) |
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i tried this w/some koh samui specimens i made 6 lc's from 2 fruit bodys. ive done lcs from spore or myc in the past and it has always worked great, this is the first time i ever tried w/a tissue sample. for some reason the only growth i am getting is like extremely off white and ulike the l.c.s i have made in the past it all kind of settled in the bottom whereas in the past the myc always kind of clouded up the whole thing... do you think this is contamination or what? i was worried about using tissue because of contamination due to tissue decay could that be what i am seeing? the sample in each jar is about the size of 1/2 a grain of rice is that too big?
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The mind is a bright blue sky
Clouds are thoughts and feelings floating by
You have them but you are not them
Whatever you do
Don't let them have you
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large_dose
Melonhead
Registered: 05/06/06
Posts: 1,346
Loc: Right in the Middle
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: fetalscab]
#6532078 - 02/05/07 01:42 AM (6 years, 3 months ago) |
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I made 3 LCs the other day, i found that my needle was too small to catch tissue from a fresh stem so i took a tiny abort and sliced the stem into thin 1/4 inch thick disks, Pc'd up some karo water and in a clean environment put the stem cuttings into the jars. Its been about 1 1/2 week and dizam i have good growth in the jars, I also did one with spores but it isnt growing nearly as quick as the fresh tissue. The myc sorta grew like a blob around the cutting at first, roping out in strands 360 degrees around le' tissue, but once i shook the jars up a lil and got the myc broken up it grew much thicker.
Edited by large_dose (02/05/07 01:43 AM)
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MajorDick
Registered: 10/14/06
Posts: 806
Last seen: 6 months, 21 days
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: large_dose]
#6532087 - 02/05/07 01:46 AM (6 years, 3 months ago) |
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Quote:
large_dose said:
I made 3 LCs the other day, i found that my needle was too small to catch tissue from a fresh stem so i took a tiny abort and sliced the stem into thin 1/4 inch thick disks, Pc'd up some karo water and in a clean environment put the stem cuttings into the jars. Its been about 1 1/2 week and dizam i have good growth in the jars, I also did one with spores but it isnt growing nearly as quick as the fresh tissue. The myc sorta grew like a blob around the cutting at first, roping out in strands 360 degrees around le' tissue, but once i shook the jars up a lil and got the myc broken up it grew much thicker.
Next time use an active pin not an abort. Pick a pin in the center of a nice cluster. There is an impressive thread from RR with pin-clones on agar.
Yeah.. this thread deserved a bump
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Thats weird.. I logged on for the first time in ages for some advice, and it looks like someone else has been using my account. WTF?
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large_dose
Melonhead
Registered: 05/06/06
Posts: 1,346
Loc: Right in the Middle
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: MajorDick]
#6532102 - 02/05/07 01:49 AM (6 years, 3 months ago) |
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my bad, it wasnt an abort, it was activly growing.
and heres fastest LC as of today..
You gotta realize how simple this is, sterile karo water and a clean environment to do the transfer. i heard of some users soaking the tissue in h202 water but i put mine right in and had no probs. Its getting pretty dense, the strain is B+, does everything look ok to you guys? This is my first lc clone attempt
Edited by large_dose (02/05/07 11:51 PM)
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large_dose
Melonhead
Registered: 05/06/06
Posts: 1,346
Loc: Right in the Middle
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: large_dose]
#6535125 - 02/05/07 11:52 PM (6 years, 3 months ago) |
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^ bump - uploaded pics.
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Sulli
Stranger
Registered: 01/31/07
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: large_dose]
#6550816 - 02/10/07 04:13 PM (6 years, 3 months ago) |
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Hey I started my LC yesterday and this will be day one. Every thing is looking good so far. PICS coming soon.
Also has anybody ever stabed their finger with the needle???
I could not get the top of it and when I did my arms did some kind of kick back and stuck the needle right into my thumb, damn it hurt so bad! Any body ever do that?
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FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,615
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: hucker696]
#7114562 - 07/01/07 12:21 PM (5 years, 10 months ago) |
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Man O Man...
WHERE HAVE YOU BEEN ALL MY LIFE!
so tell me...
WHat are your thoughts on this...?
http://www.shroomery.org/forums/showflat.php/Number/7093565#Post7093565
I know you've seen it!
you just wanted me to come find this didn't you! lol.
I know how ya'll are!
Thanks Brah.
-the Schlager-
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w. murderface
7/7/07
Registered: 06/24/07
Posts: 36
Loc: mi
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: FellowGrower]
#7158059 - 07/10/07 10:38 PM (5 years, 10 months ago) |
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Liquid Culture right?
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Eat right stay fit DIE anyways.
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sproket13
Stranger
Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 1 year, 11 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: w. murderface]
#7189474 - 07/17/07 08:32 PM (5 years, 10 months ago) |
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when you are stiring your LC using the magnetic stir plate...how do you get the magnetic stirrer out??? wouldnt you then have contams? or does none of that matter. im SLIGHTLY confused..
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shroom1957
backatit
Registered: 10/11/06
Posts: 245
Last seen: 5 years, 6 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: sproket13]
#7189837 - 07/17/07 10:05 PM (5 years, 10 months ago) |
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you put the stirrer in b4 you pc and leave it in until you're done with that LC. I use painted paneling nails....don't rust.
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Reality is just a way of looking at things that you were taught before you were old enough to know any better! -me
For me the world is weird because it is stupendous,awesome,mysterious,unfathomable;my interest has been to convince you that you must assume responsibility for being here, in this marvelous world,in this marvelous desert,in this marvelous time. I wanted to convince you that you must learn to make every act count, since you are going to be here for only a short while;in fact,too short for witnessing all the marvels of it.
-Don Juan
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Peace
Life doesn't end @ your flesh...
Registered: 05/22/06
Posts: 156
Loc: Theraputic Mountain
Last seen: 2 years, 2 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: creamcorn]
#7202698 - 07/20/07 04:05 PM (5 years, 10 months ago) |
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I have a question... will this Albertsons brand of Clover Honey Grade A work? I'm not sure if its organic or not... it just gives 'honey' as the ingredients on the back. I want to test out doing a 50/50 (karo and honey) but cant start till i know its the right honey :\
Any advice is greatly appreciated.
thanks for the tech!
Edited by Peace (07/20/07 04:13 PM)
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CaptTrip
Captain
Registered: 01/17/07
Posts: 265
Last seen: 4 years, 3 months
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Re: LC Cloning - Newbie friendly pictorial tek! [Re: Peace]
#7202871 - 07/20/07 04:53 PM (5 years, 10 months ago) |
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Quote:
Peace said:
I have a question... will this Albertsons brand of Clover Honey Grade A work? I'm not sure if its organic or not... it just gives 'honey' as the ingredients on the back. I want to test out doing a 50/50 (karo and honey) but cant start till i know its the right honey :\
Any advice is greatly appreciated.
thanks for the tech!
I've tried a couple different brands of honey and haven't noticed much of a difference.
I think it suggests "organic" honey because it is a little clearer, which helps your LC stay clearer.
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 Cambodian Grow Log
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