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 Akrobatik
Chillin
Registered: 01/16/04
Posts: 267
Last seen: 6 years, 2 months
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 Re: Super easy karo tek  [Re: Bruce3]
#2337049 - 02/15/04 01:21 PM (9 years, 3 months ago) |
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poke def, you dont want to expose the myc to any air unless you have a glove box or somthing similar.
 
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 Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,115
Loc: all up in ya
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great, but i think your thinking a little too far into it, it does not need to be this complicated, with all the surran wrap and junk, just have the lid on with an innoc hole, see the steam in the container is going to cause a positive pressure environment in the container, not allowing dirty air to enter the hole, long enough for you to throw on a piece of tape before it cools, then just innoc like ya would with a half pint when its cooled, really all this tek did is eliminate the metal lid on a jar, im not bashing you at all though, its cool.
4) microwave the container, with the lid slightly cracked(so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)
it wont melt, all of the plastic storage containers are microwave and freezer safe these days, you just need a vent for steam so the lid doesnt pop off, wich is why you just make your innoc hole in the lid before microwaving, sterilize it, take it out of the nuker, and throw on some tape over the hole asap.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
Edited by Psychoslut (02/15/04 04:59 PM)
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Nickster_154371
Hobbyist
Registered: 01/01/04
Posts: 465
Loc: 'MERICUH
Last seen: 16 days, 3 hours
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 Re: Super easy karo tek [Re: Bruce3]
#2338183 - 02/15/04 06:40 PM (9 years, 3 months ago) |
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poke through
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Drug Hit List: marijuana, mushrooms, cocaine, LSD, DMT, Ectasy, 2C family, opium, mescaline
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TinTree
thread killer(semi pro)
Registered: 02/15/04
Posts: 1,456
Loc: Mu
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Somebody asked earlier, and I'm curious too.
How long can you store the fluid before inoculation?
(First post here btw... hi everybody!)
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"I'm afraid of losing my obscurity. Genuineness only thrives in the dark. Like celery."
- Aldous Huxley
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,977
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Re: Super easy karo tek [Re: TinTree]
#2338325 - 02/15/04 07:29 PM (9 years, 3 months ago) |
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I've stored LC jars for weeks before innoculating.
SM
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,977
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nice work j...
this might make liquid culture a bit more n00b friendly. The only thing I would suggest is the addition of some peroxide.
AFTER the spores have germinated several cc's of peroxide (about 5 or so for that much water) should be addded through the innoculation hole. The peroxide prevents contams and speeds up colonization by adding 02 to the karo solution.
sM
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vermonster
Lost Sailor
Registered: 10/17/03
Posts: 205
Loc: Shangri La
Last seen: 1 year, 9 months
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Afoaf has used a 1/2 pt jar with a plastic lid for the same
idea. She has to heat it for 1 min and then let it cool for
a bit and heat it again. If she does it for 5 min straight
the innoc hole is too small too relieve excess pressure and
the top pops off. She also covers it with a coffee filter for
heating and then tyvek for incubation. For storage the jar is
covered in foil and put in the door of the fridge (shakes the jar a little).
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prstnjak
newbie
Registered: 02/13/04
Posts: 44
Last seen: 6 years, 10 months
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Re: Super easy karo tek [Re: Bruce3]
#2338782 - 02/15/04 10:03 PM (9 years, 3 months ago) |
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does anybody know any other brand of imitation light syrup???
And where I can get it Thanks!
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salazare
fan_of_shrooms
Registered: 04/29/03
Posts: 185
Last seen: 7 years, 2 days
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Re: Super easy karo tek [Re: prstnjak]
#2340583 - 02/16/04 12:20 PM (9 years, 3 months ago) |
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HI this is realy usefull and easy, thanks guys
one simple question thought, how long can this thing be good if kept to room temperature?
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salazare
fan_of_shrooms
Registered: 04/29/03
Posts: 185
Last seen: 7 years, 2 days
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Re: Super easy karo tek [Re: salazare]
#2342163 - 02/16/04 06:13 PM (9 years, 3 months ago) |
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bump
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Nickster_154371
Hobbyist
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Simplemachine, how do you know when the spores germinate? I want to inject a few cc's of peroxide to help speed things up, but im not sure what im looking for. Also, its been about 2 days since i innoced w/ spores and nothing seems to be happening...i just have a big clump of black spores floating around in the liquid and its being kept at 85-87 degreez.
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Drug Hit List: marijuana, mushrooms, cocaine, LSD, DMT, Ectasy, 2C family, opium, mescaline
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,977
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I would wait untill there is at least a little myc colonizing the jar. after maybe a week.
SM
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salazare
fan_of_shrooms
Registered: 04/29/03
Posts: 185
Last seen: 7 years, 2 days
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someone can help me about how long is this karo mycelium solution viable if stored at room temperature?
Edited by salazare (02/18/04 10:21 AM)
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,977
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Re: Super easy karo tek [Re: salazare]
#2349285 - 02/18/04 10:53 AM (9 years, 3 months ago) |
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mavix
rtfm
Registered: 01/28/04
Posts: 91
Loc: http://www.shroomery.org/...
Last seen: 8 years, 11 months
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As quoted below, I'm wondering the following, you stated that the lid is cracked open while cooking, yet it gets sealed imediately after the microwave has stopped. how does one do this without introducing contaminated air....
by following your instructions i had the following effect, I sealed the tupperware while hot. the resulting cooling of the expanded gasses inside created a vacuum.... this was then released when puncturing the saran wrap. how does one do this w/o introducing contaminants. do you do the innoculation and subsequent solution removal in your GB?
Mavix who is worried about contamns.
Quote:
angryjslice said:
3) put a peice of plastic wrap over the top of the container, and then put the lid on top of that - like this:
4) microwave the container, with the lid slightly cracked (so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)
5) let cool for a few hours, or untill it is room temperature again, and then lift up the lid slowly, flame sterilize your syringe, and put in 1 - 2cc's of your left over spore solution.
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-- mavix
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,115
Loc: all up in ya
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Re: Super easy karo tek [Re: mavix]
#2350070 - 02/18/04 02:26 PM (9 years, 3 months ago) |
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read my post above
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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angryjslice
now with 20%more anger
Registered: 10/29/03
Posts: 916
Last seen: 3 years, 5 months
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Re: Super easy karo tek [Re: mavix]
#2350090 - 02/18/04 02:29 PM (9 years, 3 months ago) |
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the lid is cracked so that the container doesnt melt, and so the plastic wrap doesnt melt into the lid. i had no trouble at all with any contaminations even though air filled back in when i injected the spore solution. ive done this many times. if your even in a semi sterile area it shouldnt be a problem. make sure you sterilize your needle though.
~JSlice~
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,115
Loc: all up in ya
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dude if you use a microwave safe dish it will not melt, im not sure about surran wrap, but the surran wrap is not needed, explain to me why you couldnt do this exactly the way you would a half pint with a plastic lid, besides the steam popping the lid off, and i posted the solution for that already.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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angryjslice
now with 20%more anger
Registered: 10/29/03
Posts: 916
Last seen: 3 years, 5 months
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Re: Super easy karo tek [Re: Psychoslut]
#2350125 - 02/18/04 02:35 PM (9 years, 3 months ago) |
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>> really all this tek did is eliminate the metal lid on a jar, im not bashing you at all though, its cool.
actually all this tek did was put pictures and a basic outline to something we had been discussing for a while that nobody had compiled into one easy to follow outline. this is my personal way of doing it. just wanted to make it easy, not 'cool'. the plastic wrap is very usefull because it acts as another contam barrier. sure you could go without it, but why? then you have to deal with making a resealable hole in the lid of your container.
>> it wont melt, all of the plastic storage containers are microwave and freezer safe these days
the containers i have say right on them "vent lid during microwaving to prevent melting" ...there the cheap ones and yes i did try to leave the lid closed during a different experiment with the same containers and the plastic caught on fire 
sorry for the double post
~JSLice~
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angryjslice
now with 20%more anger
Registered: 10/29/03
Posts: 916
Last seen: 3 years, 5 months
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Re: Super easy karo tek [Re: Psychoslut]
#2350149 - 02/18/04 02:39 PM (9 years, 3 months ago) |
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Quote:
Psychoslut said:
dude if you use a microwave safe dish it will not melt, im not sure about surran wrap, but the surran wrap is not needed, explain to me why you couldnt do this exactly the way you would a half pint with a plastic lid, besides the steam popping the lid off, and i posted the solution for that already.
use your half pints...personally, all of my half pints were in use and i needed more solution for innoc. to each there own man. its just another idea, several people have pm'd me and said this was very easy and worked out well for them.
~JSlice~
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