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Offlineparabowl
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P. Ovoideocystidiata ID Request
    #16293231 - 05/28/12 01:52 AM (11 months, 14 days ago)

Ok, so I have been finding some dark capped Ovoids (I think) and I was wondering if anybody else has seen them like this and if these could possibly be them.  I think they are and see them growing in clusters with ovoids.


I also found this huge cluster in a new spot where most of them seemed to be thicker.  I posted this in the Ovoid 2012 thread and a few people wanted me to do an ID request because they believed that this cluster might not be them.


I took and dried the cluster and this is what they look like.


I tapped the dried cluster up to a blank slide on my microscope to see if i could find any spores, I was able to get a couple.  This is 800x


Also here is a fresh non dried spore sample that I took of other previous ovoids (tested and approved :smile: ).  These were not from the cluster in question, but are a good reference.


I found a delicious chicken mushroom as well


Edited by parabowl (05/29/12 06:24 PM)


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Offlinepsylosymonreturns
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16293270 - 05/28/12 02:02 AM (11 months, 14 days ago)

why did you delete your pics ?


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Offlineparabowl
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Re: P. Ovoideocystidiata ID Request [Re: psylosymonreturns]
    #16293281 - 05/28/12 02:04 AM (11 months, 14 days ago)

I have no clue why it is showing that...I am trying to figure that out myself :confused:

Everytime I upload a picture, it uploads and when I click on it, it says that the picture has been deleted when I did no such thing...maybe the photo upload is down at the moment or something?


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Invisiblemaynardjameskeenan
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16293423 - 05/28/12 02:47 AM (11 months, 14 days ago)

It's the site, not you. I still think they are ovoids but like always wait for a TI.


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Offlinefux234
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Re: P. Ovoideocystidiata ID Request [Re: maynardjameskeenan]
    #16293735 - 05/28/12 04:58 AM (11 months, 14 days ago)

looks like some big suckers :laugh:
lets wait till the pics are available in big


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InvisibleKBG1977
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16293974 - 05/28/12 08:16 AM (11 months, 13 days ago)

Quote:

parabowl said:
I have no clue why it is showing that...I am trying to figure that out myself :confused:

Everytime I upload a picture, it uploads and when I click on it, it says that the picture has been deleted when I did no such thing...maybe the photo upload is down at the moment or something?



did you paint this?


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Offlineweiliigod
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Re: P. Ovoideocystidiata ID Request [Re: KBG1977]
    #16294064 - 05/28/12 09:07 AM (11 months, 13 days ago)

Quote:

KBG1977 said:
Quote:

parabowl said:
I have no clue why it is showing that...I am trying to figure that out myself :confused:

Everytime I upload a picture, it uploads and when I click on it, it says that the picture has been deleted when I did no such thing...maybe the photo upload is down at the moment or something?



did you paint this?





Whoever did, that' is awesome!! simply beautiful!

OP: I am fairly certain (from what I can see...) your mushrooms are active. Probably ovoids as you suspected.


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Offlineparabowl
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Re: P. Ovoideocystidiata ID Request [Re: weiliigod]
    #16301724 - 05/29/12 06:35 PM (11 months, 12 days ago)

Ok, I reuploaded the pics and they work!  I am pretty sure that the big cluster is them.  For me the spores through the microscope look identical to the ones that I know are ovoids so I am fairly sure...to be on the safe side I just wanted to see what you guys thought.  For the first two pics, I have been finding those small capped ones growing in with the clusters so I am pretty sure that those are them as well but again, just want to be sure.  I only found a couple of them but i thought they were interesting.  Thanks for all of the help so far guys!


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OfflineSomeGuy
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16301851 - 05/29/12 07:04 PM (11 months, 12 days ago)



I was going to come to this thread and say I was probably overreacting, and they are liable to be ovoids...however, I'm no spore microscopist, but I honestly think they are different


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OfflineSomeGuy
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Re: P. Ovoideocystidiata ID Request [Re: SomeGuy]
    #16301930 - 05/29/12 07:18 PM (11 months, 12 days ago)


stropharia rugoso-annulata


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OfflineNeoSporenS
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Re: P. Ovoideocystidiata ID Request [Re: weiliigod]
    #16302166 - 05/29/12 08:19 PM (11 months, 12 days ago)

I think they are just ovoids. Everything looks right, except the colors, which might look a bit off due to the flash or OP's camera setting. The veil is down low on the stipe, just like on all of mine. Plus you can see bluing on the gills in a few of the pictures. If someone is taking pictures of the spores, they should be measured.


Quote:

weiliigod said:
Quote:

KBG1977 said:
Quote:

parabowl said:
I have no clue why it is showing that...I am trying to figure that out myself :confused:

Everytime I upload a picture, it uploads and when I click on it, it says that the picture has been deleted when I did no such thing...maybe the photo upload is down at the moment or something?



did you paint this?





Whoever did, that' is awesome!! simply beautiful!

OP: I am fairly certain (from what I can see...) your mushrooms are active. Probably ovoids as you suspected.




psylosymonreturns painted that last Fall. Have you seen the one he did of Olive's cube picture?


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Offlineparabowl
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Re: P. Ovoideocystidiata ID Request [Re: SomeGuy]
    #16302190 - 05/29/12 08:24 PM (11 months, 12 days ago)

Hmm interesting pics.  I think that part of the reason that spores may look different is how the slide was prepared.  They may have used Melzer's while I just simply tapped out the cap onto a blank empty slide and put a cover plate over it dry. 

I found this old post where if you scroll near the bottom, a member put up some spores and he thought he found caerulipes but a TI actually posted a bit later and said it was ovoids,  They look nearly identical to mine
http://www.shroomery.org/forums/showflat.php/Number/6306975

Also the spore pic on wikipedia looks very convincing as well.
http://en.wikipedia.org/wiki/File:Psilocybe_ovoideocystidiata_spores.jpg


I do agree that the stropharia pseudocyanea is a possibility as you posted previously on the ovoid thread.  I wish that i would have taken a spore print but i was near certain that they were them when i picked them.  I have a hunch they are ovoids as there were ovoids growing all around them and it was about a mile upstream of my normal spot, but still I know immitators can creep in.  I dont plan on doing anything with these guys anytime soon but I would like to have some more certainty, better safe than sorry!


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Offlineparabowl
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16302219 - 05/29/12 08:29 PM (11 months, 12 days ago)

Also a note to add, those pictures were taken at night with a flash...found them just as the sun was going down right as I was about to go home.

I wish i had a ruler that small to measure them but I dont yet haha.  Maybe i will have to pick up a mini scale of some sorts for in the future.  I am pretty new into microscopy.


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OfflineNeoSporenS
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16302228 - 05/29/12 08:31 PM (11 months, 12 days ago)

Here are two more pics of Ovoid spores that I just took about two minutes ago, 800X.


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OfflineGrova
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Re: P. Ovoideocystidiata ID Request [Re: NeoSporen]
    #16302478 - 05/29/12 09:17 PM (11 months, 12 days ago)

They appear to be monstrous ovoids to me!

:peace:Grova


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Offlineparabowl
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Re: P. Ovoideocystidiata ID Request [Re: Grova]
    #16302905 - 05/29/12 10:25 PM (11 months, 12 days ago)

bfogg, thanks for taking some pics of yours.  The help is much appreciated!  I just took some more shots of mine out of curiousity...I added some water to it and it certainly changed the appearance of them a bit.  You can see those little dots inside of them that appear in pictures so they must be either water droplets or air bubbles whichever, i cant tell.  These pics are from the same exact slide as the pics that i took originally, just added water.

Here are some pics I just took at 800x
zoomed in

Here are some that I took at 2000x which were clustered onto the dry gills that broke off onto the slide.  When you add a water droplet they really spread out a bit and you can see them better. 


I am sure that if I dimmed my light a bit i would get them looking just like yours.  They look identical to the wikipedia spores.


Edited by parabowl (05/29/12 10:31 PM)


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OfflineSomeGuy
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Re: P. Ovoideocystidiata ID Request [Re: parabowl]
    #16304905 - 05/30/12 08:14 AM (11 months, 11 days ago)

here's the issue I have with it; Ovoid spores are subrhomboid and subellipsoid on end view. I see very few spores in your pics that are subrhomboid, they all appear subellipsoid. Nothing was added to those other spore pictures, yours are just that purple and ovoids are just that brown. Eat those stropharia if you want, it probably won't kill ya :lol:


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OfflineNeoSporenS
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Re: P. Ovoideocystidiata ID Request [Re: SomeGuy]
    #16305633 - 05/30/12 01:25 PM (11 months, 11 days ago)

They are NOT STROPHARIA. If anything, they look more like Leratiomyces percevalii. Maybe somebody should actually measure the spores instead of just posting pictures of them. Spores shape can be manipulated by many things, including how hard he pushes down on his cover slip, what he used to mount them (ie. water, alcohol, KOH, or whatever he uses), how hydrated they are, maturity, ect.

OP. if you don't have a reticle, or stage micrometer to use, take a picture with your pointer needle in the picture. You can then count the number of pixels for the width of the needle, and use that number to form a ratio to measure spores. Example, if the pointer is 5um wide, and has 20 pixels in a picture, than a spore that's 80 pixels in length would be roughly 20um. This ONLY works if the pictures are taken with the exact same camera, at the exact same zoom level, and only if you know the actual measurement of the pointer.

Or hey, a KOH test would help. Not many people even think of this test when out in the field, and can make for a quick ID when confused.


From here: http://www.shroomery.org/9580/Psilocybe-ovoideocystidiata
SPORES:(7–) 8–9 (–12) × (5.5–) 6–7 (–8.5) µm, rhomboid or subrhomboid in face view, subellipsoid in side view, thick walled, wall 0.8–1.5 µm thick, yellowish brown, with a broad germ pore at one end and a short appendage at the other.



Ovoids also have two separate types of Cheilocystidia and Pleurocystidia, so studying the gills under a scope would also solve this issue if more than one type is found on the specimen.


From here: http://www.shroomery.org/forums/showflat.php/Number/6910508

Psilocybe ovoideocystidiata (published description)

Pileus (10–) 15–25 (–45) mm diameter
Stipe (15–) 25–60 (–90) × (1–) 2–5 (–7) mm
Spores (7–) 8–9 (–12) × (5.5–) 6–7 (–8.5) rhomboid or subrhomboid in face view, subellipsoid in side view
Cheilocystidia of two types, a: short, 18–22 × 5–9 (–11) µm, as pleurocystidia type a; b: 25–29 × 9–13 µm, globose, pedunculate or subpedunculate
Pleurocystidia of two types, a: short, 16–24 (–35) × 6–8 (–10) µm, hyaline, ventricose-rostrate, with an acute or broad base; b: large, 20–30 (–40) × (10–) 12–16 (–20)
Annulus membraneous


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Edited by NeoSporen (05/30/12 01:26 PM)


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OfflineSomeGuy
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Re: P. Ovoideocystidiata ID Request [Re: NeoSporen]
    #16305895 - 05/30/12 02:24 PM (11 months, 11 days ago)

I apologize bfogg, I'm not trying to be inflammatory. Don't get mad, I just seek the truth. Laretiomyces percivalli never has blue stains, thats why I'm sticking with stropharia, because several do. I won't respond again in this thread. There is no where else it can go.
8 people posted in this thread, and not a single TI :Awesketch: where's workman?


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InvisibleThe Thinker

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Re: P. Ovoideocystidiata ID Request [Re: SomeGuy]
    #16306322 - 05/30/12 03:50 PM (11 months, 11 days ago)

Quote:

SomeGuy said:
8 people posted in this thread, and not a single TI :Awesketch: where's workman?



i'd call them ovoids, Psilocybe species at least


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