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 EvilMushroom666
Heretic
 
Registered: 11/18/09
Posts: 10,096
Loc: Canada 
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 Re: Flame Sterilization Experiment  [Re: Terry M]
#16086892 - 04/13/12 11:09 PM (1 year, 1 month ago) |
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Quote:
Terry M said:
Interesting experiment, EM. For case H, what was the history of the scalpel before it was wiped down with alcohol in the open air? Had it recently been flame sterilized, or allowed to sit in open air for a while?
are you referencing E?
If so the blade was left sitting on a table for...10-25 minutes, it was
so long ago I cannot remember exactly.
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 Terry M
Stranger in a Strange Land
Registered: 06/18/10
Posts: 1,354
Loc: Rhode Island
Last seen: 15 hours, 24 minutes
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Quote:
EvilMushroom666 said:
Quote:
Terry M said:
Interesting experiment, EM. For case H, what was the history of the scalpel before it was wiped down with alcohol in the open air? Had it recently been flame sterilized, or allowed to sit in open air for a while?
are you referencing E?
If so the blade was left sitting on a table for...10-25 minutes, it was
so long ago I cannot remember exactly.
Oops, yes.
Thanks for the info, EM!
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Liberté, égalité, humidité.
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spiritlands
I WAGGLE air into my trays!
Registered: 06/21/10
Posts: 638
Loc: 
Last seen: 16 hours, 7 minutes
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Re: Flame Sterilization Experiment [Re: Terry M]
#16088495 - 04/14/12 11:15 AM (1 year, 1 month ago) |
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I use 99% pure reagent grade ipa and I dont get contams hardly ever anymore. The last two Ive seen were last month, blue-green and forest green mold on agar. Both were from wild wood-lover prints. Neither have shown up since. I used bread tie wire, flame sterilized, then sprayed w/ipa (to cool it off mostly). The mold started at the inoculation point but idk if it survived or if it was on the print...
I cant say that its the alcohol exclusively. I kind of subscribed to the theory that since it evaps so fast, it fills my flow-box with heavy fumes that touch everything. It dissipates slower than it takes to evap so the fume cloud lasts a few min. I usually work right through he fumes. If you want to try some in your experiment and cant find it p.m. me.
-The flow-box is a hepa with a clean trash bag on the exhaust. It has slits for your hands and one for viewing but it blows clean air like a flow hood.
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spirits spore library
chemistry with the clown
need lab glass? Pm me.
500 ml three neck flask for sale...
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ALTERED.STATES
Biologist and NRG Tek head
Registered: 02/20/12
Posts: 356
Loc: Irish
Last seen: 1 month, 13 days
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Re: Flame Sterilization Experiment [Re: spiritlands]
#16151317 - 04/28/12 03:50 PM (1 year, 25 days ago) |
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Hi,
In science when using an inoculation loop we do not reheat the loop after it is used because it is considered contaminated and by reheating the loop doesn't go from being cool to red hot right away. There is a short time duration during heating up where whatever is on the tip will evaporate. I am sure you have seen this. You heat it, sometimes there is a popping sound with sometimes a drop of liquid squirting out. Basically whatever is on the needle can be converted into an aerosol or sometimes jump off. It is called 'sample splatter'.
The way to avoid sample splatter is to take the inoculation loop and place into disinfectant first for a few minutes before placing it again onto the flame.
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,096
Loc: Canada
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Interesting addition, thanks for the info.
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ALTERED.STATES
Biologist and NRG Tek head
Registered: 02/20/12
Posts: 356
Loc: Irish
Last seen: 1 month, 13 days
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Welcome. I would be doing this if it wasn't for the fact I just need to keep costs down and will be moving soon but will have more fun then. Nice experiment.
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,390
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Quote:
ALTERED.STATES said:
sometimes a drop of liquid squirting out.
Everything you said makes sense except this.
Do you mean a syringe, rather than an inoculation loop?
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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ALTERED.STATES
Biologist and NRG Tek head
Registered: 02/20/12
Posts: 356
Loc: Irish
Last seen: 1 month, 13 days
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Re: Flame Sterilization Experiment [Re: andymc]
#16153117 - 04/28/12 11:25 PM (1 year, 25 days ago) |
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Yes. Loops don't squirt. Needles may.
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DJ_avocado
DARK PRINCE
Registered: 11/20/09
Posts: 428
Last seen: 5 months, 6 hours
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I imagine that "sample splatter" would happen in loops as well, but not much. With syringes I wouldn't call it "splatter", more like "sample EXPLODE".
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ALTERED.STATES
Biologist and NRG Tek head
Registered: 02/20/12
Posts: 356
Loc: Irish
Last seen: 1 month, 13 days
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Re: Flame Sterilization Experiment [Re: DJ_avocado]
#16153994 - 04/29/12 03:40 AM (1 year, 25 days ago) |
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Yes, sample splatter happens with loops and syringe needles equally. Anything metalic with contamination on the end that gets heated up is subject to sample splatter, hence why the loops get disinfected. In experimental reality the loops get put in the sharps bin after every inoculation.
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Beefy1
GONE
Registered: 05/02/09
Posts: 3,573
Loc: around
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How far does this splatter travel?
I usually flame the needle outside of the box anyway, move it back in while it's still red hot.
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ALTERED.STATES
Biologist and NRG Tek head
Registered: 02/20/12
Posts: 356
Loc: Irish
Last seen: 1 month, 13 days
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Re: Flame Sterilization Experiment [Re: Beefy1]
#16154307 - 04/29/12 07:15 AM (1 year, 25 days ago) |
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Statistical mechanics. Eventually everywhere inside the inoculation chamber if you keep splattering.
In layman's terms how far does oil spit when you fry? Fry enough and the cooker plus back wall needs cleaning all over.
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DayTripin
NooB MycO GuY
Registered: 10/20/12
Posts: 299
Last seen: 10 days, 11 hours
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Quote:
TranscendingLife said:
You should be using denatured alcohol in alcohol lamps.
It's cheaper than iso, too.
Holy Shnikes Batman! Look at the size of that syringe!
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I do not produce, ingest, or distribute any illegal substances.
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