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 tripdawg420
low life with no life
Registered: 02/03/09
Posts: 1,989
Loc: illinois
Last seen: 9 minutes, 37 seconds
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 mikesethnobotany
KCUMB class of 2017
Registered: 07/29/11
Posts: 1,204
Loc: Missouri, USA
Last seen: 12 days, 6 hours
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 Re: Flame Sterilization Experiment  [Re: tripdawg420]
#15195522 - 10/08/11 01:02 PM (1 year, 7 months ago) |
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Nice idea for an experiment EM. Looking forward to some results!
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life is good
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 EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,094
Loc: Canada 
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4 days in and no sign of bacteria. I would suspect if there was any bacterial
contaminates they would have shown themselves by now. I plan to leave the
plates for another week or two just to see if any contaminate spores will
germinate.
This is by no means concrete evidence, or an experiment with constant
variables. I encourage others who have more background doing experiments
and with science to perhaps carry out more research.
There is no argument that wiping down an instrument that has been flamed
sterilized opens a vector of contamination. The question is how likely is
that vector to ruin your projects. I will update this post if anything
starts growing.
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brand0n
large jar mad man!!
Registered: 02/09/09
Posts: 842
Last seen: 24 days, 22 hours
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thanks for the update.
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That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!
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maug
Registered: 11/28/10
Posts: 1,648
Loc: inside you
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Re: Flame Sterilization Experiment [Re: igigi]
#15207812 - 10/11/11 01:32 AM (1 year, 7 months ago) |
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Quote:
igigi said:
Quote:
TranscendingLife said:
 
NICE experiment, bro!
same here
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I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,094
Loc: Canada
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Re: Flame Sterilization Experiment [Re: maug]
#15230754 - 10/15/11 08:55 PM (1 year, 7 months ago) |
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9 Days in and still no contaminate growth of any kind on any of the plates.
I plan to keep them around for another 7-10 days.
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,385
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
   How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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tripdawg420
low life with no life
Registered: 02/03/09
Posts: 1,989
Loc: illinois
Last seen: 9 minutes, 37 seconds
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Synth Ethics
Headphonaut
Registered: 05/18/09
Posts: 4,651
Loc: canada
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Re: Flame Sterilization Experiment [Re: tripdawg420]
#16034445 - 04/02/12 05:53 PM (1 year, 1 month ago) |
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so how it turned out?
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,094
Loc: Canada
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Re: Flame Sterilization Experiment [Re: Synth Ethics]
#16034452 - 04/02/12 05:54 PM (1 year, 1 month ago) |
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No contamination across the board. But remember this is a small test
that did not follow strict standards...more testing would be needed
to put it into stone.
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cosmic carnage
Adventurer
Registered: 03/01/12
Posts: 27
Last seen: 1 month, 29 days
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So the experiment didn't prove anything?
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The only person you can rely on is yourself. No hesitation!
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 4 days, 17 hours
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It proved that a flame sterilized needle is clean. If the OP repeats the experiment, he should try alcohol without flame sterilization and watch the green and slime(bacteria) appear since alcohol doesn't sanitize the inside of the needle.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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Wing
The Eye Tyrant
Registered: 04/26/11
Posts: 2,802
Loc: Middle of the East Coast 
Last seen: 3 months, 11 days
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Re: Flame Sterilization Experiment [Re: RogerRabbit]
#16037713 - 04/03/12 12:15 PM (1 year, 1 month ago) |
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Maybe work with reused needles since a lot of people save them to use later for LC and stuff.
There could be a lot of variations to test.
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My Grow Logs
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cosmic carnage
Adventurer
Registered: 03/01/12
Posts: 27
Last seen: 1 month, 29 days
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Re: Flame Sterilization Experiment [Re: Wing]
#16037733 - 04/03/12 12:20 PM (1 year, 1 month ago) |
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Thanks RR for the explanation. I'm using a glass lamp with 70% isopropyl alcohol solution to sterilize my needles. Is there a better method?
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The only person you can rely on is yourself. No hesitation!
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Wing
The Eye Tyrant
Registered: 04/26/11
Posts: 2,802
Loc: Middle of the East Coast
Last seen: 3 months, 11 days
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If you are using that alcohol to fuel a flame that flame sterilizes your needle, then no, there isn't really a better method than that.
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My Grow Logs
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,385
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Re: Flame Sterilization Experiment [Re: Wing]
#16037760 - 04/03/12 12:27 PM (1 year, 1 month ago) |
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You should be using denatured alcohol in alcohol lamps.
It's cheaper than iso, too.
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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cosmic carnage
Adventurer
Registered: 03/01/12
Posts: 27
Last seen: 1 month, 29 days
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Thanks for the correction! I was confused about alcohol flames.
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The only person you can rely on is yourself. No hesitation!
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fungal_alchemist
Ghetto-Engineer
Registered: 03/19/12
Posts: 277
Last seen: 1 day, 12 hours
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Ty for putting the time to do something like this. i know you stated earlier that you didn't do a control dish. would be interesting to see a plate that was exposed in open air for a few sec. just to see the load in your lab. Again, ty for doing the experiment
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Grocery Store Agar Tek
damion5050's coir Tek
A. augustus grow
"I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape."
Paul Stamets
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,094
Loc: Canada
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Perhaps at a later time I may go into things a bit deeper and more structured,
but for now I am far to busy.
Everyone please feel free to conduct your own experiments.
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,390
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Re: Flame Sterilization Experiment [Re: tripdawg420]
#16039823 - 04/03/12 08:50 PM (1 year, 1 month ago) |
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The hypothesis, and the point, is still a good one though
- A flame-sterilized whatever (scalpel, syringe, etc) is sterile.
- Alcohol-soaked whatevers (swabs, paper towels, etc) are almost certainly not sterile - and the alcohol won't make them so.
- Don't touch a sterile thing with a non-sterile thing; doing so will make it non-sterile, almost by definition.
Flame-sterilize, then go to work. You can't do better than sterile. Sterile is 100%.
Alcohol is certainly useful for sanitizing things, and you should definitely sanitize any relevant item you can't sterilize. You can't sterilize your gloved hands, for example, so we do the next best thing, and sanitize them with alcohol.
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How I make spore prints
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If he asks me "Did you have a good time?" I'll say,
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