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 Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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 LC Lid Question? 
#13779162 - 01/13/11 10:58 AM (2 years, 4 months ago) |
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Do LC lids need a vent port? I know it sounds like a dumb question but I was just wondering. If you had no vent port you could turn ur LC jar up side down and it woud make getting the culture out, easier. just a thought.
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"Mushrooms always grow better when you're not looking!" ~Larynx
 
(Everything posted by this account is 100% fictional.)
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 Doc_T
Random Dude
 
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Re: LC Lid Question? [Re: Larynx]
#13779182 - 01/13/11 11:08 AM (2 years, 4 months ago) |
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If there's enough air in the jar, you're ok- but it's easier to harvest if there's a vent hole.
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You make it all possible. Doesn't it feel good?
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hamloaf
The Lone Pine Cone.
Registered: 12/23/09
Posts: 8,214
Loc: Oklahoma City.
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Re: LC Lid Question? [Re: Larynx]
#13779194 - 01/13/11 11:13 AM (2 years, 4 months ago) |
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Quote:
Larynx said:
Do LC lids need a vent port? I know it sounds like a dumb question but I was just wondering. If you had no vent port you could turn ur LC jar up side down and it woud make getting the culture out, easier. just a thought.
The vent port you speak of is referred to as a gas exchange filter port.
Turning a liquid culture upside down will wet the gas exchange filter material and contaminate your culture.
Whatman syringe filters are the best gas exchange filters for liquid cultures because you can shake the culture without getting the filter wet.
Synthetic filter disks, tyvek/micropore tape, and polyfill can all be used efficiently as a gas exchange filter for cultures.
When using the fore-mentioned filter material for gas exchange to your liquid culture, be sure when agitating your culture to break it up, don't get the filter material wet.
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Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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Re: LC Lid Question? [Re: hamloaf]
#13779222 - 01/13/11 11:22 AM (2 years, 4 months ago) |
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Quote:
The vent port you speak of is referred to as a gas exchange filter port.
Turning a liquid culture upside down will wet the gas exchange filter material and contaminate your culture.
Whatman syringe filters are the best gas exchange filters for liquid cultures because you can shake the culture without getting the filter wet.
Synthetic filter disks, tyvek/micropore tape, and polyfill can all be used efficiently as a gas exchange filter for cultures.
When using the fore-mentioned filter material for gas exchange to your liquid culture, be sure when agitating your culture to break it up, don't get the filter material wet.
I know, that is why I am asking? If you didnt need a vent port, you could turn the jar upside down and not worry about getting the material wet. Just one hole in the lid with RVT silicone. turn it upside down, and no leaking, no wet filter, and it makes getting the myco wad out easier.
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"Mushrooms always grow better when you're not looking!" ~Larynx
(Everything posted by this account is 100% fictional.)
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N2DFlood
Fractalist
Registered: 11/10/10
Posts: 389
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Re: LC Lid Question? [Re: Larynx]
#13779254 - 01/13/11 11:30 AM (2 years, 4 months ago) |
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I make my LC's without vents but with RTV ports, I pull air through a hot needle and give them 10cc's every week or so, if pressure builds up i vent them with an open syringe and give them another 10cc's.
There should be enough air in the jar to get them going if you vent them with an airport syringe to relieve the initial vacuum. Once grown out they go in the fridge and get the weekly dose.
-Flood
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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Re: LC Lid Question? [Re: N2DFlood]
#13779309 - 01/13/11 11:43 AM (2 years, 4 months ago) |
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My LC Lids:
Or the Simplified Version: 
Here's what I got after a week of inoculation: 
That last picture is actually of an ELME + agar LC...Jokefox gave me the idea. He's making a tek & it seems to work pretty well.
Here's the tek I followed for the lids: http://www.shroomery.org/forums/showflat.php/Number/13626890#13626890
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
   How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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idunno
PinkWebBuffalo
Registered: 03/02/10
Posts: 1,087
Last seen: 1 year, 9 months
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Re: LC Lid Question? [Re: Larynx]
#13779322 - 01/13/11 11:46 AM (2 years, 4 months ago) |
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Cant suck out liquid/air in a vacume- Thus the use for a gas port-
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The death of one man is a tragedy. The death of millions is a statistic.. Josef Stalin
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Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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Re: LC Lid Question? [Re: idunno]
#13779495 - 01/13/11 12:29 PM (2 years, 4 months ago) |
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Quote:
idunno said:
Cant suck out liquid/air in a vacume- Thus the use for a gas port-
Good Fvcking point
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"Mushrooms always grow better when you're not looking!" ~Larynx
(Everything posted by this account is 100% fictional.)
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FractalXplora
Grainiack
Registered: 02/11/06
Posts: 2,353
Loc: UK 
Last seen: 1 day, 20 hours
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Re: LC Lid Question? [Re: Larynx]
#13779601 - 01/13/11 12:57 PM (2 years, 4 months ago) |
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get creative with your lids, i found 3ml syringes can be transfomed into sunken withdrawal tubes to stop tipping it up with harvesting.
Doc T style port sorta!
Thanks Doc, had two of those red caps lying about, works great.
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Morelman
LC Master
Registered: 05/17/06
Posts: 1,010
Loc: Between Right and Wrong
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Re: LC Lid Question? [Re: Larynx]
#13779624 - 01/13/11 01:01 PM (2 years, 4 months ago) |
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Check out my Journal.
No need to tilt the jar with my design.
If you use syringe filters. Get the ones with a PTFE Hydrophobic
Membrane.
Thanks todlow!! Good to see others using it....
Edited by Morelman (01/13/11 01:11 PM)
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Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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Re: LC Lid Question? [Re: idunno]
#13779647 - 01/13/11 01:06 PM (2 years, 4 months ago) |
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Quote:
idunno said:
Cant suck out liquid/air in a vacume- Thus the use for a gas port-
Couldnt you inject a feww cc's a serilized water into the LC to create preasure, and then withdraw the culture with he same syringe?
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"Mushrooms always grow better when you're not looking!" ~Larynx
(Everything posted by this account is 100% fictional.)
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Morelman
LC Master
Registered: 05/17/06
Posts: 1,010
Loc: Between Right and Wrong
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Re: LC Lid Question? [Re: Larynx]
#13779698 - 01/13/11 01:15 PM (2 years, 4 months ago) |
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Quote:
Larynx said:
Quote:
idunno said:
Cant suck out liquid/air in a vacume- Thus the use for a gas port-
Couldnt you inject a feww cc's a serilized water into the LC to create preasure, and then withdraw the culture with he same syringe?
Yes, but eventually you will dilute the nutrient value. Sterilized nutrient broth injected first would be ideal.
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Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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Re: LC Lid Question? [Re: Morelman]
#13779724 - 01/13/11 01:19 PM (2 years, 4 months ago) |
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Good idea, inject more LC into the jar instead of water. BTW, Morelman I really like your lids. I am definitly gonna use those when i get a chance. So expect a PM from in the very near future. Thanks again guys
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"Mushrooms always grow better when you're not looking!" ~Larynx
(Everything posted by this account is 100% fictional.)
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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Re: LC Lid Question? [Re: Morelman]
#13779770 - 01/13/11 01:27 PM (2 years, 4 months ago) |
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Quote:
Morelman said:
Check out my Journal.
No need to tilt the jar with my design.
If you use syringe filters. Get the ones with a PTFE Hydrophobic
Membrane.
Thanks todlow!! Good to see others using it....
 Yea, when I first saw them I was like  those are retarded! then I bought some Whatmans (300 actually, yes they'll be for trade soon) & let loose. I didn't like all the additional hardware you had on the original lids & decided to go a little different way, especially the rubber stoppers. So I ditched the turkey flavor injector & rubber stoppers. Check out my pics. You can see how I built mine. Here's a pic of how I sliced & bent my piping so it wouldn't fall through my hole.
 
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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hoobatech
facilitator
Registered: 08/16/04
Posts: 275
Last seen: 16 hours, 45 minutes
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Re: LC Lid Question? [Re: Larynx]
#13779861 - 01/13/11 01:39 PM (2 years, 4 months ago) |
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if its the vacuum your worried about, couldn't you fill a syringe body with poly-fill and then stab your injection port, that would draw filtered air in to equalize the pressure
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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Re: LC Lid Question? [Re: hoobatech]
#13779872 - 01/13/11 01:41 PM (2 years, 4 months ago) |
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yes. seen people do it in a SAB/GB, but it's risky.
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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hugger-mugger
Registered: 10/14/09
Posts: 200
Loc: Dallas
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See my thread here: http://www.shroomery.org/forums/showflat.php/Number/13760246
Thats what happens if you have a vacuum.
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Morelman
LC Master
Registered: 05/17/06
Posts: 1,010
Loc: Between Right and Wrong
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That's Cool!! 
If you had a small flaring tool you could probably make it clean and neat. Don't know if they have one small enough to grip a 5/32" tube though.
I've seen small flared nipples at hardware stores before so there must be a way to do it.
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Larynx
The Jungian
Registered: 06/15/10
Posts: 242
Loc: Guess You'll Never Know
Last seen: 7 days, 23 hours
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Re: LC Lid Question? [Re: Morelman]
#13780338 - 01/13/11 03:08 PM (2 years, 4 months ago) |
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So you could have 2 holes with RVT on them. Stick one with a polyfil syring to get rid of the vaccum. Then, stick the serile syringe in the other hole turn up side down and withdraw the myco. That sounds simple nd all you need is RVT and an extra syringe.
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"Mushrooms always grow better when you're not looking!" ~Larynx
(Everything posted by this account is 100% fictional.)
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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Re: LC Lid Question? [Re: Larynx]
#13780351 - 01/13/11 03:12 PM (2 years, 4 months ago) |
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I'd recommend just putting in a GE hole. It makes it a lot easier & less troublesome.
Morelman - yea, I just used my dremel tool to cut it in the center then some pliers to bend it out more. It could be better. I tried to use the dremel tool to flare out the end, but it didn't really work. I'm going to get a smaller cutter to flare out the end.
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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