Ha, I just was talking about that on another thread....

I'm going to cut and paste a little something to save myself some time here .....

Bear with me [ ]  I tossed a quick reference answer under the quote if you dont want to read through all of it.....

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ProfessorPinHead said:


The saran wrap seals the top to help retain humidity and the Polyfil both helps retain humidity and allows filtered gases to exchange during colonization. This helps reduce the likelihood of contams entering.


Here is an example.
This tub colonized all the way from 0% -> 100% under an ambient light cycle with nothing but Saran wrap, polyfil, and a 30 gallon black trash can liner under the substrate.





.... No outer bag, no duct tape!!!!!  THIS IS OUT OF DATE MISINFORMATION!!!!!!



I know there are a few different opinions on this one going around to date....
This process takes fine tuning to get down. I have been doing it for years and this is what I have learned to be true. I am a scientist and hold a research degree from a major university. I have been cultivating fungi since biology class during my undergraduate degree. This is my advice......

Do with it what you wish

-prof.

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For any noobs reading this; asking themselves .... We use the black liner to keep light out from under the substrate and to keep our growth medium clean.

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hamloaf said:

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For any noobs reading this; asking themselves .... We use the black liner to keep light out from under the substrate and to keep our growth medium clean.

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The black trash bag liner is also used to encourage moisture evaporation through out the middle of the substrate's surface.  Moisture evaporation encouragement through out the middle of the substrate's surface discourages side pinning often created between the walls of the fruiting chamber and substrate where micro-climate is often more optimal without the use of the liner.

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ProfessorPinHead said:
Yea, once its at 100%, Saran comes off, tub gets misted once / day.

At this point we want evaporation of the substrate off the surface area and high FAE rates so as to trigger pinning.


FAE is the # 1 pinning trigger.

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Thanks. I'll definitely try this polyfill/saran wrap way next & update this post with my outcome or reference yours on my own post.

I used your opinion on my Coir brick/verm/gypsum/coffee grounds & it seems to be working great.  The tub I PMed you with only a few was my fault mainly, but there are two HUGE PE porkers that came out. I want to clone them.... I've read that poking syringe through middle of one & squirting the inner flesh into a LC type jar of Karo would do this?

-EDIT-
Pic of HUGE porkers:

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ProfessorPinHead said:
Yea, that will work. You need to be rather sterile though.

Use a flavor injector needle. The biopsy is hard to work with if you use a 16-18 gauge needle. You can just swap out the needles pre/post cloning. I just sterilize the turkey needle in the PC with the LC fluid so it is ready to use when you take your tissue biopsy.

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ProfessorPinHead said:
Yea, that will work. You need to be rather sterile though.

Use a flavor injector needle. The biopsy is hard to work with if you use a 16-18 gauge needle. You can just swap out the needles pre/post cloning. I just sterilize the turkey needle in the PC with the LC fluid so it is ready to use when you take your tissue biopsy.

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sawbagz said:
Awesome tek. I recently used damions 50/50 coir tek but am going to use your recipe next time once I can get my dirty mits on some poo. Im planning on using RR's pasteurization tek though as I have everything needed already/

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Yea, Its easier. If you just leave your stove on the warm setting it is usually 170 degree which is perfect.

Glad I could be of help!

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boredcertified said:

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ProfessorPinHead said:
Yea, that will work. You need to be rather sterile though.

Use a flavor injector needle. The biopsy is hard to work with if you use a 16-18 gauge needle. You can just swap out the needles pre/post cloning. I just sterilize the turkey needle in the PC with the LC fluid so it is ready to use when you take your tissue biopsy.

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Y did I not think of that?
KUDOS
BC

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Kuz, we did for you