Isolating a strain on agar - Agar and Liquid Culture

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Mycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 8 years, 8 months

Re: Isolating a strain on agar

[Re: Azmodeus]
#1337022 - 02/26/03 08:15 AM (10 years, 2 months ago)

Have you had some peroxide to your agar ?

With peroxide agar, sometime it takes me more than 8 days to see the first sign of growth.
But normal PDY agar show mycelium growth after 4-6 days.

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Azmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 8 years, 4 months

Re: Isolating a strain on agar

[Re: Mycolaureat]
#1337072 - 02/26/03 08:51 AM (10 years, 2 months ago)

No i did not add peroxide this time......
I'll wait a day or two then check all teh petris... :crazy:

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"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "

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Anonymous

Re: Isolating a strain on agar

[Re: Azmodeus]
#1342393 - 02/28/03 09:12 AM (10 years, 2 months ago)

Okay guys this is a lil off topic but it falls under agar isolation. My friends isolates have a bacterial contam. The mycelium is growin rather well with the contam all around it. I took some straight 3% H2O2 and wrinsed of the plates. It seemed to have worked rather well. The isolates are spreading faster now. And the bacteria has dwindled away. Now time for next sectoring.
Has any one had any experience with this same situation? the man also has some agar made up with h2o2 in it but it has solidified what can he do to liquify it with out desturbing the h2o2 in it?
peace

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

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