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 Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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 Wild Strain Isolation - Print to Print **complete**(1/16) 
#1238096 - 01/21/03 10:35 PM (10 years, 4 months ago) |
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This log will document the steps necessary to isolate a wild strain from print to print.
I obtained a wild print in a trade. The print was taken from a non-sterile fruit.
The following are steps in one particular procedure. Many modifications can be made to get to the same endpoint.
The first step in isolation is to streak a plate with the spores. This method consists of taking an inoculation loop and transfering spores from the print to the surface of an agar plate. I used I scalpel for this step and an agar plate with an antibiotic mixed in. I tend to inoculate the plates at points rather than streaking. Streaking requires pulling the inoculation loop across the surface in a sweeping motion. The agar used was Malt Extract Agar w/ .05 g gentamycin/ liter. The gentamycin prevents bacterial growth. I use gentamycin because is survives higher temperatures, other antibiotics tend to break down at the temps reached in my PC.
This is what the plate looked like after 5 days according to my records (which are probably faulty as this is much more growth than should be expected after 5 days.) This growth is very nice considering it came from a wild print.
I will give out more info regarding the origin of the strain after I talk to the user that provided the strain to me. I will be adding steps as frequently as time allows. Currently this strain is much further along than I have shown here. It is now colonizing millet.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (01/16/04 02:03 PM)
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G a n j a
Pictish and proud
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Posts: 7,316
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 Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1238133 - 01/21/03 11:38 PM (10 years, 4 months ago) |
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Thanks for this joshua this will be intereesting to follow 
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er
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 stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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 Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1238238 - 01/22/03 02:35 AM (10 years, 4 months ago) |
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Nice!
I will follow this thread...closely.
Is it what i think it is? 
peace...
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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 Re: Wild Strain Isolation - Print to Print [Re: stonErollEr1]
#1238531 - 01/22/03 05:46 AM (10 years, 4 months ago) |
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Yes it is. Please keep the info to yourself until I announce the specifics. I'd like to talk to the provider of the print regarding the naming of this strain.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1239005 - 01/22/03 09:18 AM (10 years, 4 months ago) |
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Sure will...bro!
peace..
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Seril
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1239417 - 01/22/03 11:44 AM (10 years, 4 months ago) |
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exciting! 
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Seril,
Political language -true of all political parties- is designed to make lies sound truthful and murder respectable, and to give solidity to pure wind..
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angryshroom
Stranger
Registered: 12/18/01
Posts: 7,262
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 Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1239469 - 01/22/03 11:57 AM (10 years, 4 months ago) |
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Yes, very cool indeed
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Dymentia
journeyman
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1240226 - 01/22/03 04:04 PM (10 years, 4 months ago) |
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what is this all about?
Quote:
Is it what i think it is?
Quote:
Yes it is. Please keep the info to yourself until I announce the specifics.
the suspense is killing me, i must know!
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debianlinux
Myconerd - DBK
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Re: Wild Strain Isolation - Print to Print [Re: Dymentia]
#1240313 - 01/22/03 04:37 PM (10 years, 4 months ago) |
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ok, i'm hooked, it's obviously believed to be a new strain.
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blahblahblah
Mad Scientist
Registered: 10/25/01
Posts: 1,002
Loc: South America
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1240663 - 01/22/03 06:48 PM (10 years, 4 months ago) |
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Quote:
I will give out more info regarding the origin of the strain after I talk to the user that provided the strain to me. I will be adding steps as frequently as time allows. Currently this strain is much further along than I have shown here. It is now colonizing millet.
I hope you have some more pictures of the petris and such, THAT is what I want to see
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Joshua
Holoman
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Posts: 5,380
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Re: Wild Strain Isolation - Print to Print [Re: blahblahblah]
#1242178 - 01/23/03 08:39 AM (10 years, 4 months ago) |
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To isolate a strain is also called sectoring. After the original inoculations grew out I chose areas of healthy/vigorous growth and transfered squares of the healthy sections to a new plate. These are pics of the first transfer grown on Dog Food agar.
I think this one is smiling at all of you Shroomerites!
It is always best to work with multiple plates, as some may succumb to contamination. It also increases your chances of finding a healthy strain as some isolates will perform better than others.
The branching you see is an indication of healthy growth. This type of growth is called rhizomorphic growth. Rhizomorphic growth grows faster and forms hyphal knots more readily than cottony growth. The rhizomorphic growth is good and is what I am looking for. I would pick the best growth off of these plates and transfer them to new plates.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (01/23/03 08:45 AM)
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G a n j a
Pictish and proud
Registered: 12/03/02
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1242436 - 01/23/03 10:17 AM (10 years, 4 months ago) |
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I'm in the process of trying this for the first time so thank you for this.
Very clear pictures.How long did it take for the first plate to fill up like that and at what temp?
Cheers
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er
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@cro
new name
Registered: 12/07/02
Posts: 1,224
Loc: The PNW
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1242438 - 01/23/03 10:17 AM (10 years, 4 months ago) |
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Those are some nice lookin rhizos there, if this is going to be a new strain I can see it quickly becoming popular. Good Work, have fun.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
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Re: Wild Strain Isolation - Print to Print [Re: G a n j a]
#1243747 - 01/23/03 06:10 PM (10 years, 4 months ago) |
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The growth you see in those plates is probably 5 days old.
Here is a petri of a different isolation I am working on as well. I am showing this one to show the difference of what a good isolation and a not-so-good isolation may look like.
This was the growth from the initial inoculation.
This is the growth after 2 transfers.
I hope you can see the difference in the rhizomorphic growth between the two isolates.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,316
Loc: Zone ate
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1244149 - 01/23/03 08:52 PM (10 years, 4 months ago) |
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Yep big difference
i can also almost read what you blacked out in picture 1 lol 
but only almost  lol
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er
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Alkaloids
3,4,5-trimethoxyphenethylamine
Registered: 11/16/98
Posts: 627
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1244204 - 01/23/03 09:20 PM (10 years, 4 months ago) |
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Good work as usual Joshua. I'll be following this as I am going to post something similar in the near future.
What are those container you are using? The agar tranfers look unusually thick.
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Joshua
Holoman
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Re: Wild Strain Isolation - Print to Print [Re: Alkaloids]
#1244216 - 01/23/03 09:24 PM (10 years, 4 months ago) |
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I have polypropylene and Pyrex dishes. The polypropylene are quite a bit deeper than regular petris. I like to pour my plates thicker than most. Perhaps w/ more experience I will pour them thinner and conserve on supplies.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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ShrewDigsby
Toker
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Loc: Shrewtown somewhere near ...
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Re: Wild Strain Isolation - Print to Print [Re: G a n j a]
#1244238 - 01/23/03 09:33 PM (10 years, 4 months ago) |
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This is an awesome log...just like all the rest 
I want to learn, I want to learn
Ganja, you have great logs too, you should post your latest.
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Marijuana is a horticultural plant. Hemp is an industrial weed. I believe they were both provided to us by GOD to use and enjoy.
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stonErollEr1
The Psilocybinsolution
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Re: Wild Strain Isolation - Print to Print [Re: ShrewDigsby]
#1244511 - 01/24/03 12:48 AM (10 years, 4 months ago) |
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Looking nice Joshua.
Very healthy rhizos there.
Peace..
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1244542 - 01/24/03 01:24 AM (10 years, 4 months ago) |
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I think some points should be raised here.
Firstly Antibiotics such as gentamycin dont actually stop bacterial contaminants but they can limit their prevelance. Bacterial as opposed to molds which are more of a problem, appear as liquidy spots on the plate. Because you are streaking the spores across the plate you should be able to quickly get some germination points that dont display any bacteria which you can simply transfer to h202 agar.
Antibiotics will not effect the germination of contaminants like everyone's favuorite green molds (Pencillium etc.) and as these have spores, they can quickly spread and contaminate an entire plate.
As Joshua has stated, that is why it is important to innoculate five or six plates and quickly transfer to h202 agar to kill contaminant spores and clean up the culture. From there you can isolate rhizomorphic sectors.
The last point i wanted to make is that rhizomorphic mycelia does not guarantee strong fruiting performance. With cubensis once the plate is fully colonised and exposed to light, it will fruit. Even if you isolate some sectors that seem linear or even cottony, they may perform better than the rhizomorphic mycelia that Stamets loves so much - there is a post regarding this issue in advanced forums right now. So test every isolate - nice looking mycelia or otherwise. Good practise as most mushrooms wont fruit off agar - youll never be able to test this with Hy.Capnoides or Lepista Nuda.
BTW - most cubensis prints ive received that ive streaked on agar have contaminated worse than wild prints. Due to the stagnent nature of fruiting chambers im assuming that there are very, very few cubensis that are grown in sterile environments. My point being that if you can grow out normal cubensis on agar, you can grow out wild varieties just as easily.
This goes for pan cyans as well, they are pretty easy to domesticate to fruit well - easier than the weird Ps.Eucalypta and Ps.Stuntzi ive been working with.
--------------------
Holidaying in San Diego...
Edited by bluemeanie (01/24/03 01:27 AM)
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Joshua
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Re: Wild Strain Isolation - Print to Print [Re: bluemeanie]
#1248200 - 01/25/03 03:14 PM (10 years, 4 months ago) |
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"Firstly Antibiotics such as gentamycin dont actually stop bacterial contaminants but they can limit their prevelance."
It is my understanding that gm prevents growth of certain species. The prevalence of bacteria is limited due to the fact that only certain species can grow in the presence of the drug.
The F+ strain I isolated is an example of a non-rhizomorphic isolate. I think the points Stamets makes regarding the supriority of rhizomorphic growth have to do with the speed of growth as well as the observation that rhizomorphic strains tend to initiate hyphal knots faster and at a higher rate than cottony growth. I do believe there can be exceptions to any observation. I will check out your thread in advanced.
__________________________________________________
I took the healthiest growth from the first transfer and moved it to new plates. One of my plates from this 2nd transfer is shown below. The plate was absolutely beautiful!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1249304 - 01/26/03 03:30 AM (10 years, 4 months ago) |
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From most of my experience gentamycin only limited the size and arrival of bacterial contamination.
Your right about stamets but he does tend to suggest that rhizomorphic mycelia is required for strong fruiting, despite later explaining that only two in three spore matches are capable of fruiting.
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Holidaying in San Diego...
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Joshua
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Re: Wild Strain Isolation - Print to Print [Re: bluemeanie]
#1256682 - 01/28/03 08:20 PM (10 years, 4 months ago) |
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I looked for the thread you posted, would you give me the title or a link?
Here are some pics of a couple of different isolates from this strain colonising white millet.
Here is a pic of an isolate in 8% honey water.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1256778 - 01/28/03 08:52 PM (10 years, 4 months ago) |
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Holidaying in San Diego...
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Joshua
Holoman
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Posts: 5,380
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Re: Wild Strain Isolation - Print to Print [Re: bluemeanie]
#1264226 - 01/31/03 05:09 AM (10 years, 4 months ago) |
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"nice to have something other than cubensis logs once and a while."
I hate to disappoint you.
The next person to reply gets to choose a name for this strain. The original print came from St. Thomas in the US Virgin Islands.
Choose one of the following names please:
St. Thomasian (my pick)
US Virgin Islander
West Indian (The Virgin Islands are also known as the West Indies)
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
JazzMatazz
addict
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1264277 - 01/31/03 05:49 AM (10 years, 4 months ago) |
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Virgin Islander it is!
(Wow , I actually influenced Mycohistory!  )
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Perception is limited to consciousness.Expand it and unfold other realities.
Edited by JazzMatazz (01/31/03 05:50 AM)
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Papa_Smurf
member
Registered: 11/17/01
Posts: 163
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 Re: Wild Strain Isolation - Print to Print [Re: JazzMatazz]
#1264346 - 01/31/03 06:26 AM (10 years, 4 months ago) |
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damn! missed west indian by a half hour
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"Only after you have lost everything, are you free to do anything"
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X.O
Fucktard
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Post deleted by Moe Howard [Re: Joshua]
#1264372 - 01/31/03 06:35 AM (10 years, 4 months ago) |
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I'm a huge idiot
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
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Re: Wild Strain Isolation - Print to Print [Re: X.O]
#1266012 - 01/31/03 03:50 PM (10 years, 4 months ago) |
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west indian would have been better. Virgin Islands is no good.
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Holidaying in San Diego...
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GGreatOne234
Stranger
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1266276 - 01/31/03 06:27 PM (10 years, 4 months ago) |
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St. Thomas
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angryshroom
Stranger
Registered: 12/18/01
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Re: Wild Strain Isolation - Print to Print [Re: Joshua]
#1266452 - 01/31/03 07:54 PM (10 years, 4 months ago) |
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How bout St. Angryshroom... It has a nice ring to it!!
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X.O
Fucktard
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Post deleted by Moe Howard [Re: bluemeanie]
#1268753 - 02/01/03 05:05 PM (10 years, 4 months ago) |
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I'm a huge idiot
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BladeLSD
Squirrel Master
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Re: Wild Strain Isolation - Print to Print [Re: X.O]
#1270425 - 02/02/03 08:59 AM (10 years, 4 months ago) |
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How about Mary Tudor strain to recognize the Virgin queen of England. Or Virgin Queen. 
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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity 
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X.O
Fucktard
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Post deleted by Moe Howard [Re: BladeLSD]
#1270540 - 02/02/03 09:35 AM (10 years, 4 months ago) |
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I'm a huge idiot
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blahblahblah
Mad Scientist
Registered: 10/25/01
Posts: 1,002
Loc: South America
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Re: Wild Strain Isolation - Print to Print [Re: X.O]
#1271335 - 02/02/03 03:52 PM (10 years, 4 months ago) |
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Quote:
no
AH HA HA HA HA! Dont let him down easy, just give it to him straight god dammit
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the free thinker
salesman
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Re: Wild Strain Isolation - Print to Print [Re: blahblahblah]
#1271561 - 02/02/03 05:36 PM (10 years, 4 months ago) |
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My vote goes for West Indian.
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Why not Rasta-totting Jah-Chile' big-arsed, hoe-spanking, slutty-nipples strain?
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Holidaying in San Diego...
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X.O
Fucktard
Registered: 11/21/02
Posts: 1,449
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Post deleted by Moe Howard [Re: bluemeanie]
#1272138 - 02/02/03 09:44 PM (10 years, 4 months ago) |
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--------------------
I'm a huge idiot
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: Wild Strain Isolation - Print to Print [Re: X.O]
#1272193 - 02/02/03 10:08 PM (10 years, 4 months ago) |
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The naming of this strain is not up for debate from my perspective. It has been named.
Please do not post any more posts regarding this issue w/i this thread.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (02/02/03 10:09 PM)
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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US Virgin Islander [Re: Joshua]
#1287921 - 02/07/03 08:37 AM (10 years, 4 months ago) |
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Here are fully colonised quart jars containing white millet. I tried to show how fluffy the myce4lium was on top but the autofocus on my camera focused on the glass. I think you can still see the fluffiness.
I spawned some dung a few days ago and it contamed. I just spawned all three of these jars into Mycota compost w/ nutrient solution. We will see how things go from here.
This log is now real-time.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Birdman
If you catch onfire, do notrun!
Registered: 11/28/00
Posts: 153
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 Re: US Virgin Islander [Re: Joshua]
#1288225 - 02/07/03 10:56 AM (10 years, 4 months ago) |
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I'll be following this post closely. I'll be taking quit a few trips to St. Croix during the next couple years and hope to find some good prints. Joshua...Thanks for the great info!!
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"Livin' a Juggalo's life"
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Seril
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 Re: US Virgin Islander [Re: Birdman]
#1292763 - 02/09/03 08:25 AM (10 years, 4 months ago) |
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looks great!
let us know how the spawn run turns out!
--------------------
Seril,
Political language -true of all political parties- is designed to make lies sound truthful and murder respectable, and to give solidity to pure wind..
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: Joshua]
#1305016 - 02/13/03 09:35 AM (10 years, 4 months ago) |
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Here is another isolation I have been working on. I just did some g2g (grain to grain) transfers and will be making a small casing w/ the remaining spawn.
Of the three jars spawned previously one remains.
Two were mixed into Mycota compost together. This spawn run seems to have developed a bacterial infection. The other jar was spawned into compost alone and has completely colonised the surface of the compost. I will remix the top surface back into the remaining compost. I'll explain more in a bit. I have to head to class now.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
|
Re: US Virgin Islander [Re: Joshua]
#1306242 - 02/13/03 05:43 PM (10 years, 4 months ago) |
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I am back from class now. I checked on the supposed bacterial infected substrate. I guess I was just a bit over cautious. The substrate is colonising and no odor is present. Here's a pic.
As far as the other casing. I didn't have enough spawn for all of the substrate I prepared. So I spawned the top couple of inches with what I had. I figured this would create a barrier to contamination and once the layer was colonised I would mix it back into the entire volume of substrate. I will do some mixing later tonight w/ some more pics maybe.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
|
Re: US Virgin Islander [Re: Joshua]
#1307532 - 02/14/03 08:22 AM (10 years, 4 months ago) |
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Here are pics of the other casing. They show the complete surface colonisation and the substrate after mixing the surface back in.
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
|
Re: US Virgin Islander [Re: Joshua]
#1320263 - 02/19/03 06:41 PM (10 years, 3 months ago) |
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The casing w/ inadequate spawn has contaminated. No doubt it was my lack of asceptic technique that caused its demise.
The other casing has not progressed much since I last looked at it. I am going to place it in cooler temps to see if it does better. The last time I thought is was contamed I placed it in my kitchen @ 68 F and when I looked at it after a couple of days it had taken off. Perhaps I have a cube that prefers lower temps. I will post a pic as soon as my upload allowance is refreshed.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
SmashedMe
Stranger
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Re: US Virgin Islander [Re: Joshua]
#1320850 - 02/20/03 03:09 AM (10 years, 3 months ago) |
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Can't wait to see the fruits.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: SmashedMe]
#1321462 - 02/20/03 08:43 AM (10 years, 3 months ago) |
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Me neither!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
blackout
Microwaver
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Re: US Virgin Islander [Re: Joshua]
#1323935 - 02/21/03 06:32 AM (10 years, 3 months ago) |
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great guide. like bluemeanie says, many traded prints are contaminated, so this would be useful to anybody trading prints. should be guides like this on the fsre and sticky threads on the spore exchange board. when using trade prints i now take as few spores as possible to lessen the chance of contams. i only start them on agar so i dont get annoyed if they contam since i only loose agar and not a big bag of millet
are you sure it is cubensis then Joshua? it would be nice to get one that likes the cold.
why did you use 8% honey water? i know honey is not 100% sugar but 8% seems a lot
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Bavet
Sensitive StonedRebel
Registered: 12/12/02
Posts: 383
Last seen: 1 year, 3 months
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Re: US Virgin Islander [Re: blackout]
#1324151 - 02/21/03 08:31 AM (10 years, 3 months ago) |
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Very interesting log hope you get some great fruiting.
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"~Dream as if you'll live forever....live as if you'll die today~ James Dean"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: blackout]
#1324522 - 02/21/03 11:23 AM (10 years, 3 months ago) |
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I miscalculated my honey %, it should have been 4%. I did even worse on a more recent batch and added 12%...oops.
I am not sure of anything...I steer clear of cubensis and other psychoactive fungi due to their illegal nature.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: Joshua]
#1331727 - 02/24/03 02:31 PM (10 years, 3 months ago) |
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A bucket of fun. USVI on Mycota compost mixed w/ 25% verm by volume, hydrated with a 10 drops/gallon Mycota nutrient solution.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: Joshua]
#1350860 - 03/04/03 08:52 PM (10 years, 3 months ago) |
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Allrighty...I finaly have a casing in the chamber of magic. The other casings contaminated. This one did not.
There are hyphal knots present. The casing was placed into the chamber on...damn I forgot! I think it was 2/28.
Here is the start of a new strain! This strain will not be available for a bit though. I am going to print at least 100 prints first. These prints will then be donated to the FSRA and FSRNW...stay tuned!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,316
Loc: Zone ate
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Re: US Virgin Islander [Re: Joshua]
#1350897 - 03/04/03 09:13 PM (10 years, 3 months ago) |
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Looking good
hey be nice to your european relatives and send the F.s.r.e a couple too lol
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er
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ATWAR
Connoisseur
Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
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 Re: US Virgin Islander [Re: Joshua]
#1350920 - 03/04/03 09:25 PM (10 years, 3 months ago) |
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Thats some mighty fine demonstration you have there ! Extremely informative, and good reading.
Any possibility that shroomery members can "reserve" a print? New strains intrigue me...
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To give is to live...
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: G a n j a]
#1350925 - 03/04/03 09:28 PM (10 years, 3 months ago) |
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I have a European associate who will be amplifying the spores with me before the strain release. He will be donating all of his spores from this strain to the FSRE.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (03/04/03 09:41 PM)
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Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: ATWAR]
#1350946 - 03/04/03 09:42 PM (10 years, 3 months ago) |
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Keep your eye on this thread. The release date for this strain will be announced here.
3/7 early AM
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (03/07/03 01:48 AM)
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Joshua
Holoman
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Re: US Virgin Islander [Re: Joshua]
#1356944 - 03/07/03 10:06 PM (10 years, 3 months ago) |
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3/8 Early AM
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,580
Loc: Snoqualmie, Wa.
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 Re: US Virgin Islander [Re: Joshua]
#1356952 - 03/07/03 10:09 PM (10 years, 3 months ago) |
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Houston we have pins!!!
Nice bro...good luck with the grow!!!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with  on there names, that means they have mushrooms for sale.
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Jackal
Well Versed InEtiquette
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Re: US Virgin Islander [Re: Roadkill]
#1357006 - 03/07/03 10:55 PM (10 years, 3 months ago) |
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Sweet, this is getting exciting!
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gee_bone
Seeker FromNature
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Re: US Virgin Islander [Re: Jackal]
#1362039 - 03/10/03 07:08 AM (10 years, 3 months ago) |
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Lets go,
time for more pics and an update on this little treasure....
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Dissidents are punished far more by themselves than by society.
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Birdman
If you catch onfire, do notrun!
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Re: US Virgin Islander [Re: gee_bone]
#1363279 - 03/10/03 03:23 PM (10 years, 3 months ago) |
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Houston...I have a woody!
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"Livin' a Juggalo's life"
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Joshua
Holoman
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US Virgin Islander-Things just got better!! [Re: gee_bone]
#1364196 - 03/10/03 10:30 PM (10 years, 3 months ago) |
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Here's the requested update:
Early AM 3/11
I also have a new use for an old Hydrapod.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
gee_bone
Seeker FromNature
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1368463 - 03/12/03 09:13 AM (10 years, 3 months ago) |
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Thanks for the update Josh.
Hey if i was finding shrooms out in East Coast Canada, what strain would they be?
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Dissidents are punished far more by themselves than by society.
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Joshua
Holoman
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1370492 - 03/12/03 07:44 PM (10 years, 3 months ago) |
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3/12 late PM
Do not use clear containers unless you have to.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1385892 - 03/17/03 09:19 PM (10 years, 3 months ago) |
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The first flush was extremely sad
Here are the two fruits I harvested. My Brasilian casing is in the same fruiting chamber and is not having any trouble fruiting. I hope this strain performs better in the future.
3/13
3/14
Here are pics from my modified Hydrapod. These are USVI also.
3/14
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1387197 - 03/18/03 08:50 AM (10 years, 3 months ago) |
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Here are some pics from a moment ago (3/18).
Flush #2 beginning...hopefuly it will be more impressive this time around. I will be sure to pamper it.
(USVI=US Virgin Islander)
Here is a pic from the Hydrapod. I hope these grow well...if they do I will have prints galore!
(USVI)
Here is another strain isolation from a Floridian specimen. I call this strain Floridian Gold (FG). Thanks Opus!
3/18 FG
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (03/18/03 08:52 AM)
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Joshua
Holoman
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1448830 - 04/11/03 06:05 AM (10 years, 2 months ago) |
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here are some recent USVI pics. The fruits seem to be small and the strain seems to flush poorly. I will attempt growing the strain out on a different substrate to see if it helps any.
Here are some Florida Gold specimens.
These are even smaller...like spaghetti.
The lack of a good flush on both these strains may be due to high CO2 in my chambers. I have been trying some new chambers out. It takes time to fine tune them. Hopefully this is the case and further flushes will show these strains worthy of public release.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (04/11/03 06:11 AM)
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Paid
Pict
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Re: US Virgin Islander-Things just got better!! [Re: Joshua]
#1454227 - 04/13/03 05:49 AM (10 years, 2 months ago) |
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Second flush was better :-) still a bit long and weedy looking shrooms there though.Do you think its because there not domesticated yet?Have you had sim results from other wild prints?
Good log though :-)
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BigJohnson
Whoa! You guysneed to lightenup!
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Re: US Virgin Islander-Things just got better!! [Re: Paid]
#1454434 - 04/13/03 11:00 AM (10 years, 2 months ago) |
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Awesome
--------------------
Should the US relegalize drugs?
http://www.lp.org/issues/relegalize.html
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Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander-Things just got better!! [Re: Paid]
#1455117 - 04/13/03 07:18 PM (10 years, 2 months ago) |
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Yes...sometimes the isolate doesn't perform well. That is why it is good to make several isolates.
I am more inclined to think that my poor results are do to the isolate rather than the substrate...time will tell.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: Joshua]
#1575179 - 05/24/03 10:08 PM (10 years, 29 days ago) |
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I have been working with two isolations...up to now. I have a third on its way.
So far the USVI strain puts out nothing but spaghetti shrooms...very thin, long, small caps, and overall a poor fruiting isolation. Back to the master print with that one.
The Florida Gold is a bit better. It seems to thrive in poor conditions better than good ones...strange. It puts out tall and thin mushrooms as well, it yeilds much better, but nothing to write home about. They are very pretty specimens in a kind of not so pretty way...and they blue extremely.
Here are some pics of the Florida Gold strain.
StonE-
Your prints are printing right now.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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stonErollEr1
The Psilocybinsolution
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Re: US Virgin Islander [Re: Joshua]
#1575937 - 05/25/03 08:18 AM (10 years, 28 days ago) |
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Allright..
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MuShi_KiNg
---------|)
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Love da pics :> g00d post
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anotherdirtywop
shmuck
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Re: US Virgin Islander [Re: MuShi_KiNg]
#1576739 - 05/25/03 08:02 PM (10 years, 28 days ago) |
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Excellent work joshua.....I really enjoy reading your posts!
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Mycena
mycoexplorer
Registered: 05/02/03
Posts: 270
Loc: indonesia
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Hi joshua
Youve done a comprehensive pictorial of this process and for that this is a valuable thread and refreshingly interesting
Im just wondering if in youre enthusiasm youve overlooked the purpose in isolation and development on new strain, or improving old strains
Its like any plant or animal breeding - you are looking for an improvement or a special characteristic that makes this variety unique
Wild prints are genetically variable and offer a lot of material to work with, sometimes too much for a simple ones step or F1 selection
Reasons you might want to generate a new spore race might include form, potency, yield, unusual shapes and sizes.
I use spore races like B plus, malabar, penis envy, Argentines and Amazonians as examples.
Or simply as a geographic interest - such as locating a cubensis from Cuba for example, or a strain fruiting on the geographic or chronological limits of distribution
This might have the advantage if you were able to clone a specimen growing happily during a very cool period or a cool climate.
For example near where i live there are fields full of colonised cowpats that sit dry and dormant for many months springing to life when its both warm and wet. If i was to find a cluster of fruits out of season when it is quite cold (below 15 degrees) then this may indicate a cold temperature mutant
And selection is very strict - You need to start off with an idea of what you hope to achieve and be ruthless. It may take several thousand inocculations to come up with a truly unique isolate and one that has superior fruiting characteristics.
Any old cubensis isnt going to do growers any favours - especially newbies
Using cows as an analogy - you keep only the best studs the rest you dont allow to reproduce or you end up with 'mickey' bulls and undo years of good herd management
Something you might like to think about before releasing this strain is getting it up to standards of ease of fruiting and yield as whats currently available
And properly characterising the strain so that it can be identified from 'mystery' cubes
A recent thread with one of these mystery strains ended up clearly being a Malabar.
Suggestions can make on improving this strain include many more spore innoculations on agar followed by selection of isolates judged by in vitro pinning
followed by harsh culling based on yield and speed of colonisation at set temperatures and then taking these isolates and doing at least 4 generations of multispore innoculations to inbreed them sufficiently to be able to fix the characteristics of the strain - all the time culling for poor vigour
F5 is still a bit early, F10 (tenth generation) would be much better.
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Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: Mycena]
#1582021 - 05/27/03 06:11 PM (10 years, 26 days ago) |
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Why do you think there aren't any prints from either of these strains available....for exactly the reasons you set forth.
It is good to know you understand genetic management.
This thread is far from over!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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ebola8p
Stranger
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Re: US Virgin Islander [Re: Joshua]
#1582052 - 05/27/03 06:20 PM (10 years, 26 days ago) |
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[quoteThe Florida Gold is a bit better. It seems to thrive in poor conditions better than good ones...strange. It puts out tall and thin mushrooms as well, it yeilds much better, but nothing to write home about. They are very pretty specimens in a kind of not so pretty way...and they blue extremely.
Those look exactly like the ones we find in the fields around here
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Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: ebola8p]
#1582618 - 05/27/03 10:09 PM (10 years, 26 days ago) |
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As the name suggests, the original print came from a fruit from Florida. Where are you from?
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Mycena
mycoexplorer
Registered: 05/02/03
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Re: US Virgin Islander [Re: Joshua]
#1584856 - 05/28/03 02:10 PM (10 years, 25 days ago) |
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Sorry Joshua not attacking you personally, my comments were in response to the nature of this thread, as i said its very useful one
So how do you plan to further select this spore race?
BTW that 'Florida Gold' does look like a promising spore race
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Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: Mycena]
#1585118 - 05/28/03 03:22 PM (10 years, 25 days ago) |
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I know you weren't. I think you may have underestimated my knowledge though. Sometimes we don't know how someone understands something until a dialogue is reached. I wholeheartedly welcome any appropriate dialogue. What we talk about will be added to the knowledge of this growlog. It is the knowledge I wish to pass onto others.
I'll start from the original multispore again. I have a friend who isolated some huge pins from USVI. I am cautious as the plates weren't labeled and he was working with some other isolates at the same time.
In the end it may be that this spore race likes to grow just as the isolates have been growing.
We'll see how it goes.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: Joshua]
#1596887 - 05/31/03 08:57 PM (10 years, 22 days ago) |
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Here is a large Florida Gold specimen picked this morning.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: US Virgin Islander [Re: Joshua]
#1600536 - 06/02/03 05:40 AM (10 years, 20 days ago) |
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A handfull of Florida Gold grown on horse dung compost.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: Joshua]
#1621597 - 06/09/03 07:43 PM (10 years, 13 days ago) |
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Some more FG were picked today.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
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Re: US Virgin Islander [Re: Joshua]
#1631666 - 06/13/03 05:42 PM (10 years, 9 days ago) |
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A nicely formed FG specimen.
And a handfull from a couple of days ago.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
oO_wombat_Oo
Stranger
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Re: US Virgin Islander [Re: blackout]
#1638419 - 06/16/03 10:47 PM (10 years, 6 days ago) |
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I have a collection of wild prints from around my local area, maybe someone should do all this shit with them - as long as I get to name the strain.
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Interesting development. Those cubies from Florida are quite nice. I have no problem with an attempt to domesticate a variety of cubensis that performs as well as that - simply because it might blow some of the existing varieties out of the water.
I do find though - and this is not aimed at Joshua - that for a long time vendors have been 'domesticating' wild specimens of cubensis from any exotic area they can find purely to try and capitalise on it. The whole point of developing a good, properly domesticated variety of cubensis would be due to its high yielding/fruiting performance or perhaps an oddity of its genetics that is fairly stable that would make it unique in potency, etc.
The concept of one man going to a small island in thailand and picking seven cubensis specimens from areas within a 50km radius, bringing them back to USA and having someone isolate them once or twice, posting a picture of one of the isolates that manage to fruit in a unique way, printing this and then marketing it as an excitng new 'strain' of cubensis is misleading and redudant. Or capitalism at its worst.
Cubensis like Treasure Coast, PRs, many of the thai spore-races, some of those from Australia (i like the tassie though but there is no way cubensis was found in tasmania!!!) are just average wild cubensis mushrooms being sold with poorly domesticated variable genetics. Basing their relevance as a distinct strain purely on the location of the origin mushroom. And capitalising on the wide variety of genetic throwbacks - such as TC's albino freaks as something other than what they really represent - poor selection and domestication.
If the principle being used is correct, i could go out into the Australian country side and find four cubensis fruits in four different paddocks, isolate them a little, get a few weird fruiting characteristics that i take a picture of, then sell them as four different cubensis strains. Now that is bordering on nasty!
The best example of this came from Captain Max who actually had the guile to receive a bunch of wild prints from Australia and actually use these to make syringes - the Wollongong variety was on the market no longer than two weeks after he received the original prints.
Reputable vendors are few and far between.
--------------------
Holidaying in San Diego...
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Joshua
Holoman
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Re: US Virgin Islander [Re: bluemeanie]
#1641408 - 06/18/03 04:20 AM (10 years, 4 days ago) |
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I agree. Although, I think the intentions of some of those involved have been good. I hope to offer any future strains I domesticate for free, the mushrooms have tought me this. Strain developement of our friends lacks the science that is required, and the proper resources. I do think there is a higher ideal to obtain and that we are all learning together.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
mjshroomer
Sage
Registered: 07/22/99
Posts: 13,774
Loc: gone with my shrooms
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Re: Wild Strain Isolation - Print to Print [Re: debianlinux]
#1642388 - 06/18/03 03:09 PM (10 years, 4 days ago) |
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Hey, wqhen I click on your photo it opens up to a yello page witht he shroomry info on it.
What gives?
mj
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: US Virgin Islander [Re: Joshua]
#1643123 - 06/19/03 12:58 AM (10 years, 4 days ago) |
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Im glad. The intentiosn may have been in the right place, but the profiting makes me question that.
--------------------
Holidaying in San Diego...
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Here's a nice FG specimen just picked.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
angryshroom
Stranger
Registered: 12/18/01
Posts: 7,262
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Re: Florida Gold [Re: Joshua]
#1680536 - 07/02/03 04:19 PM (9 years, 11 months ago) |
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Joshua your mushrooms are always huge
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Boppity604
Stranger
Registered: 10/19/01
Posts: 1,056
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Re: Wild Strain Isolation - Print to Print [Re: mjshroomer]
#1680702 - 07/02/03 05:21 PM (9 years, 11 months ago) |
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>>Hey, wqhen I click on your photo it opens up to a yello page witht he shroomry info on it. What gives?
That happens a lot when you click on older images. Might have to do with where the images are stored or could just be a problem with the links on the thumbnails themselves. *shrug*
Love & Light,
Boppity
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Boppity604
Stranger
Registered: 10/19/01
Posts: 1,056
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Re: Florida Gold [Re: Joshua]
#1680716 - 07/02/03 05:25 PM (9 years, 11 months ago) |
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>>Here's a nice FG specimen just picked.
Hey Joshua, why did you pick this particular specimen before the cap leveled off? I just read through this thread for the first time today...so forgive me if I missed something obvious. I would think you could get a better print off the cap once it was opened further.
Love & Light,
Boppity
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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I'm not taking prints from these. I have had a mite problem and don't want it to spread to anyone else. This isolate does not perform as well as I'd like either.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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USVI [Re: Joshua]
#1758806 - 07/29/03 07:38 AM (9 years, 10 months ago) |
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Here are the results from new isolation...supposedly from USVI, but I must question my friends petri labeling as there was no labeling for a while and he was working with several isolations. The results are pretty good nonetheless.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Dobie
Dopeless Hopefiend
Registered: 08/15/02
Posts: 50,795
Loc: ON DA BLOCK 
Last seen: 1 hour, 27 minutes
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Re: USVI [Re: Joshua]
#1759022 - 07/29/03 09:26 AM (9 years, 10 months ago) |
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damn that one is narly looking
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This place is gayer than when the balls touch
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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Re: USVI [Re: Dobie]
#1759381 - 07/29/03 01:11 PM (9 years, 10 months ago) |
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You?re getting there..Keep it up, looking better and better.
peace..
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Jackal
Well Versed InEtiquette
Registered: 10/16/02
Posts: 4,563
Last seen: 3 months, 9 days
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Some freakish stipes going on there.
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u4ia
enthusiast
Registered: 01/23/03
Posts: 240
Last seen: 6 years, 3 months
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hollow stems in that tub?
I've had stems like that, not really hollow, but definately not nice and dense like some of the others.
Just wondering what that is REALLY called.
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Procrastination is like masturbation, it's fun until you realize you are only screwing yourself.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: u4ia]
#1786721 - 08/06/03 11:51 PM (9 years, 10 months ago) |
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Certain species of mushroom grow with hollowed stems. The mushrooms in that picture had pretty thick stems for their size.
I would call the stems "gnarly".
Here are pics from the second flush.
More pics to come soon.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (08/06/03 11:52 PM)
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: Joshua]
#1786971 - 08/07/03 01:18 AM (9 years, 10 months ago) |
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Here are the additional pics. These were taken a little less than 12 hours after the last pics.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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AlienPrimate
Brachiator
Registered: 06/19/03
Posts: 110
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Re: USVI [Re: Joshua]
#1838085 - 08/21/03 06:49 PM (9 years, 9 months ago) |
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Joshua...
Any updates? I love this thread!
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I said "no" to drugs but drugs just aint takin no for an answer!
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whole9
LOVE ME BITCH
Registered: 04/28/03
Posts: 3,265
Last seen: 7 years, 1 month
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dam thats insane!
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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I've been working quite a bit on my outdoor projects. This one has not progresses very fast. My plans consist of new agar inoculations and further tests. I have a new wild print from Brasil I will also be working with soon. There are some pictures in my outdoor cultivation thread of the USVI isolate as well. Thank you very much for your encouragement...I do appreciate it.
Here are a couple of pictures of some USVI pins, soon to be fruits.
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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Re: USVI [Re: Joshua]
#1842875 - 08/23/03 09:19 AM (9 years, 9 months ago) |
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Looking good as allways josh...liooks like you got the USVI going great outdoors.
peace..
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BrainFarmer
Farmicist
Registered: 03/06/03
Posts: 544
Loc: lounging betwixt lobes...
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Re: USVI [Re: Joshua]
#1975064 - 10/03/03 05:23 AM (9 years, 8 months ago) |
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any updates?
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Gate Gate Paragate Parasamgate Bodhisvah
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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I would have had some beautiful pics from my last flush, but my camera was out of town.
There was a three gallon container with a forest of shrooms ranging from 8-12 inches tall. Each one was straight as an arrow with white fuzz growing well up the stem. At the top of each stem was the most symmetrical cap. It was a beautiful sight indeed. This particular isolate may be worth some more experimentation.
I will have pics of the second flush for you.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (10/03/03 06:00 AM)
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Here are some pics from a first flush USVI.
Damn! it was a pain to pick all of these pins!
Here are some pics from a third flush of USVI.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Anonymous
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Re: USVI [Re: Joshua]
#1987319 - 10/07/03 05:48 PM (9 years, 8 months ago) |
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Somebody set us up the bomb.
 , as always.
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M1chae1M
Curious
Registered: 09/03/03
Posts: 12
Last seen: 8 years, 5 months
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Re: USVI [Re: daba]
#1987411 - 10/07/03 06:18 PM (9 years, 8 months ago) |
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Joshua have you eaten or tested any of these shrooms ? What about that huge stem the size of your arm. haha crazy.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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I have eaten them...they taste kind of bitter .
Here is an updated pic. I'll have a couple more when my upload allowance refreshes.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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I sent you some prints a while back didn't I? If so, what strain did I send and what were the results?
I meant this to be a PM, I guess the public wanted to know as well.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (10/08/03 05:18 AM)
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@cro
new name
Registered: 12/07/02
Posts: 1,224
Loc: The PNW
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Re: USVI [Re: Joshua]
#1989195 - 10/08/03 05:20 AM (9 years, 8 months ago) |
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That is an awesome little group you got there, beautiful characteristics. Hope the internal's just as nice as the external.
Peace, @cro
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: @cro]
#1989215 - 10/08/03 05:34 AM (9 years, 8 months ago) |
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I'll save it for you if you'd like.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Anonymous
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Re: USVI [Re: Joshua]
#1989326 - 10/08/03 07:18 AM (9 years, 8 months ago) |
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are those shrooms on steroids or what!
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@cro
new name
Registered: 12/07/02
Posts: 1,224
Loc: The PNW
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Re: USVI [Re: Joshua]
#1992304 - 10/09/03 05:41 AM (9 years, 8 months ago) |
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Hmm... quite the tempting offer.
But I'm saving all I got for freaknight . My first time to one of these events in about a year and a half. Can't wait, balls to the walls. Anyways, that is an awesome looking strain your working with. I want to see more of your logs, this forum ain't the same without your shit.
Peace - @cto
Edit: Fuckin drunk ass, can't even spell my own name write
Peace [Email]-@cro[/Email]
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Edited by @cro (10/09/03 05:43 AM)
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Seril
Stranger
Registered: 01/28/02
Posts: 246
Loc: PNW
Last seen: 7 years, 4 months
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Re: USVI [Re: @cro]
#1995915 - 10/10/03 03:08 AM (9 years, 8 months ago) |
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FANTASTIC!!
beautiful pictures, as always... good camera, good operator.
I'll be checking in every so often :]
-seril
ARNOLD IS THA GOVERNATOR!!
(oh god help us all...)
--------------------
Seril,
Political language -true of all political parties- is designed to make lies sound truthful and murder respectable, and to give solidity to pure wind..
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: Seril]
#2035713 - 10/23/03 12:13 PM (9 years, 7 months ago) |
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Prints are being released!!!
This will be the fourth flush off of this 2 gallon casing.
10/23/03 3am
10/24/03 12am
10/24/03 12pm
10/25/03 9am
10/27/03 10pm
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (10/28/03 01:10 AM)
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: Joshua]
#2049207 - 10/28/03 01:30 AM (9 years, 7 months ago) |
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This grow log is complete!!!!
Print to print!!!!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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Re: USVI [Re: Joshua]
#2049644 - 10/28/03 04:10 AM (9 years, 7 months ago) |
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Yehaaa..*congratulates*
peace..
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Ben_Stein
Spelling Gestapo
Registered: 08/16/03
Posts: 370
Loc: Hooterville
Last seen: 8 years, 10 months
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Just Amazing!!
Fantastic grow and super job with the log!
You, sir, rock!
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I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames
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Tremor1127
Mental Member
Registered: 08/10/03
Posts: 3,402
Loc: In a Van Down By the Rive...
Last seen: 10 months, 21 days
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Post deleted by Administrator [Re: Ben_Stein]
#2052193 - 10/29/03 12:38 AM (9 years, 7 months ago) |
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ISH
PsilocybianTranslationServices
Registered: 03/02/02
Posts: 787
Loc: Planet Earth
Last seen: 1 year, 28 days
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Great, what was the weight on that flush?
Will you be trading any of these prints? If so PM me I'm interested. I love the look of these mushrooms rigth before the veil separates. Beautifull!
ISH
--------------------
Join The Shroomery Folding @ Home Team!
I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
- Terence McKenna, Archaic Revival
Edited by ISH (10/29/03 04:55 AM)
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Lawrence
member
Registered: 08/24/03
Posts: 321
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Re: USVI [Re: ISH]
#2053069 - 10/29/03 09:00 AM (9 years, 7 months ago) |
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Awesome ! great
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Sorry about the delay in updates. I'm just now getting my projects back to normal.
Here is a USVI casing I am currently working with.
Pin City!!!
Enjoy!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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Re: USVI [Re: Joshua]
#2246267 - 01/16/04 04:10 PM (9 years, 5 months ago) |
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Looking really promising Joshua..
peace...
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angryjslice
now with 20%more anger
Registered: 10/29/03
Posts: 916
Last seen: 3 years, 6 months
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nice...a friend of mine has a few half pints and a few full pints and some liquid culture going of the USVI strain that was won in a contest. looks promising so far. ill keep ya'll updated.
~JSLice~
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llamaboy
the weasel thatsnagged the bee
Registered: 11/08/03
Posts: 563
Loc: Portland PNW
Last seen: 3 years, 7 months
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Re: USVI [Re: Joshua]
#2246935 - 01/16/04 08:43 PM (9 years, 5 months ago) |
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i love how the cap of the mush is so spherical before the veil breaks...i've never seen a cube so perfect...great work, and great isolations
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ChromeCrow
one ancient mutha
Registered: 02/21/02
Posts: 1,831
Loc: Hoosier HELL
Last seen: 4 days, 18 hours
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Re: USVI [Re: Joshua]
#2250215 - 01/18/04 12:26 PM (9 years, 5 months ago) |
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very nice joshua....... excellent work as ALWAYS!
btw, what digi are you using?
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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A two year old Sony DSC-p30. It is a 1.3 Mp, but produces very nice pics and has excellent macro quality.
I can't make very good prints from it though. To make decent prints, I suggest getting a 3 Mp or better.
This camera works perfect for my web based work
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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Re: USVI [Re: Joshua]
#2258745 - 01/21/04 03:33 AM (9 years, 4 months ago) |
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OMG...I did not expect this. There are two days difference between these pics. I am taking prints from these...free in the Contest forum soon.
Keep in mind that this flush had no air exchange until very late in the flush, perhaps that is why it took off so fast!
Because of the holiday I stayed away from home an extra day. It seems that I would have been in a better situation w/o the holiday.
The spore deposit in my fruiting chamber was thick!! huge areas of solid spores were present.
1 17 04 pm
1 19 pm
This whole area was snow white before...
Check out the contest forum!!!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (01/21/04 03:36 AM)
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MycoGlowFlow
between the walls
Registered: 05/20/01
Posts: 1,102
Loc: michigan
Last seen: 4 years, 11 months
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Re: USVI [Re: Joshua]
#2258822 - 01/21/04 04:05 AM (9 years, 4 months ago) |
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whoa dude, excellent job with taming "florida gold" maybe one day ill find my own strain of cubensis. pls read the PM i send u..
excellent mycological publication.
some things amaze me...
--------------------
(the key-strokes that have completed everything ever typed on this user name have been a random coincidence, except for the words in this notation)
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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No shit!!
I thought that pics was of a mycelial culture.
I thought maybe Tampanensis...but then MJ...whoa.
Our perception is just that...perception!
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
MycoGlowFlow
between the walls
Registered: 05/20/01
Posts: 1,102
Loc: michigan
Last seen: 4 years, 11 months
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Re: USVI [Re: Joshua]
#2258851 - 01/21/04 04:27 AM (9 years, 4 months ago) |
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ya i appreciate that picture because of the uncanny ability of the trichomes of the plant to apear as myclium shoots, quite similar to cubensis coming thick thru a casing layer...
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 3 years, 2 months
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That was a lot och trichs..damn..
peace..
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Shpongloid
WBS-o-phile
Registered: 11/16/02
Posts: 196
Last seen: 4 years, 10 months
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HOLY SHIT...
Look at the MJ! That stuff could send someone into a coma!
Oh, by the way, great job Joshua! I love that pic of the two shrooms with dangling veils.
--------------------
"...NIDA-supplied marijuana cigarettes weigh approximately .9 grams. A smoker would theoretically have to consume nearly 1,500 pounds of marijuana within about fifteen minutes to induce a lethal response."
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