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Anonymous
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 Re: The Importance of Gas Exchange  [Re: Anno]
#1228136 - 01/18/03 06:33 AM (10 years, 4 months ago) |
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8-12 days? what do you do to make that happen?
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 Anno
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Re: The Importance of Gas Exchange [Re: ]  1
#1230572 - 01/19/03 08:15 AM (10 years, 4 months ago) |
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I prepare a substrate with the correct moisture content, use a foil lid, inoculate with relatively fresh spores and incubate at 80?F.
Edited by Anno (01/19/03 11:03 AM)
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dr4g0n
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Re: The Importance of Gas Exchange [Re: Anno]
#1230807 - 01/19/03 10:17 AM (10 years, 4 months ago) |
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thanks 100x anno. I will definatly try this in the future as my Thai's stalled for no apperent reason. It seemed they had plenty of humidity, however they were completly sealed.
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dr4g0n
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Re: The Importance of Gas Exchange [Re: happygrins]
#1230809 - 01/19/03 10:18 AM (10 years, 4 months ago) |
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I do have a question however, aren't you worried about contams slipping in along with new air?
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BladeLSD
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Re: The Importance of Gas Exchange [Re: dr4g0n]
#1230815 - 01/19/03 10:23 AM (10 years, 4 months ago) |
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Anno, I just innoculated my jars, and the lids are on fairly tight with a tinfoil layer over the lids, should I unscrew the lids a tad, and possibly remove the tape from the holes. The reason Im asking is because im not sure if your talking about 1/2 pint jars
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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity 
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Anno
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Re: The Importance of Gas Exchange [Re: dr4g0n]
#1230817 - 01/19/03 10:23 AM (10 years, 4 months ago) |
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No, they are stopped by the vermiculite layer.
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Anno
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Re: The Importance of Gas Exchange [Re: BladeLSD]
#1230830 - 01/19/03 10:29 AM (10 years, 4 months ago) |
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>should I unscrew the lids a tad
Yes.
> and possibly remove the tape from the holes.
Yes.
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BladeLSD
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Re: The Importance of Gas Exchange [Re: Anno]
#1230837 - 01/19/03 10:33 AM (10 years, 4 months ago) |
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Your the best Anno  , I heard the best way to unscrew the lids is to attach a piece of tape to the top, and the screw ring is this correct.
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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity
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Anno
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Re: The Importance of Gas Exchange [Re: BladeLSD]
#1230850 - 01/19/03 10:40 AM (10 years, 4 months ago) |
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Frankly, I don?t know, I never used the 2 piece lids.
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BladeLSD
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Re: The Importance of Gas Exchange [Re: Anno]
#1230872 - 01/19/03 10:53 AM (10 years, 4 months ago) |
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its all good, you still get 5 shrooms from me. 
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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity
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motaman
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Re: The Importance of Gas Exchange [Re: Anno]
#1231032 - 01/19/03 12:25 PM (10 years, 4 months ago) |
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Thank you Anno for posting this. Seeing how I am new to this hobby this has answered some of my questions. So seeing how this topic has already been started I hope you don't mind if I ask you or anyone else a question or two. I have some pint jars I innoculated with B+. I am using metal lids with the inners turned seal up They are on snug but not tight. There is 4 holes punched in top that were use for innoclulating. I currently have the holes covered with 3M Micropore tape(used for dressing wound and offers breathablilty. I think). They have been incubating for three days and now I see growth. Should I just remove the tape completely?
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Anno
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Re: The Importance of Gas Exchange [Re: motaman]
#1231185 - 01/19/03 01:53 PM (10 years, 4 months ago) |
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I?d leave it on for now, since it should provide some air exchange due to it structure, and the lids are inverted.
Should the jars at some point stop growing without apparent reason, take the tape off.
As I said, I had the best results with aluminum foil lids.
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motaman
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Re: The Importance of Gas Exchange [Re: Anno]
#1231629 - 01/19/03 06:01 PM (10 years, 4 months ago) |
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Thanks Anno.. I would of rathered used foil but was out at the time and all I had was 3m micropore tape. 
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Angry Mycologist
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Re: The Importance of Gas Exchange [Re: Loop_Theorist]
#1231681 - 01/19/03 06:17 PM (10 years, 4 months ago) |
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Quote:
Tell me you did NOT just question Anno's methods...
Yea, I mean, it's not like we would want anybody thinking for themselves or anything 
As far as gas exchange goes during colonization, I've had no problem with slow growth due to the extra layer of foil on top of the lid.
Also, I love the fact that they breath in oxygen and release carbon dioxide....sounds familiar. Symbiotic relationship indeed. 
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 Alien
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Post deleted by Administrator [Re: Anno]
#1231699 - 01/19/03 06:21 PM (10 years, 4 months ago) |
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-Alien
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motaman
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Re: The Importance of Gas Exchange [Re: Alien]
#1231711 - 01/19/03 06:26 PM (10 years, 4 months ago) |
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makes me wonder why i could't put a plant in my fruiting chamber.
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deanofmean 
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 Re: The Importance of Gas Exchange [Re: motaman]
#1231732 - 01/19/03 06:33 PM (10 years, 4 months ago) |
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who said you can't 
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motaman
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Re: The Importance of Gas Exchange [Re: deanofmean]
#1231826 - 01/19/03 06:59 PM (10 years, 4 months ago) |
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I haven't seen anywhere saying you can. But it sure would even out the gas exchange Not just in the fruiting chamber Why couldn't I put a few heat loving plants in my incubator at night to help with the exchange.. Anno?
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Edited by motaman (01/19/03 07:04 PM)
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Bi0TeK
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Re: The Importance of Gas Exchange [Re: Anno]
#1232082 - 01/19/03 08:21 PM (10 years, 4 months ago) |
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I Might get flamed here cos I've just skim read this post cos I'm very tired and want to get some sleep but I've ALWAYS used foil on my drinking glasses/ jars & have never used lids & have found that if you do use foil & stack your glasses/jars ontop of each other the jars ontop are ALWAYS the fastest to colonize.
Just trying to prove Anno's point about air exchange.
Goodnight !
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Edited by Bi0TeK (01/19/03 08:25 PM)
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Anno
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Re: The Importance of Gas Exchange [Re: motaman]
#1232143 - 01/19/03 08:44 PM (10 years, 4 months ago) |
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>Why couldn't I put a few heat loving plants in my incubator at night to help with the exchange
Plants convert CO2 and water into oxygen and sugars....when they have light. This process is called photosynthesis.
The incubator shouldn?t have light. So this woudn?t really work well.
Plants need some oxygen for themselves too, btw.
Read
http://www.mcps.k12.md.us/departments/eventscience/EBS.OS1_RS~DF.html
http://www.hsv.k12.al.us/schools/middle/wtms/student/cell/cell_energy.html
http://www.saburchill.com/chapters/summary02.html
Besides, the point is not that the oxygen isn?t available, it is there , in the normal air, to more than 20%, the problem is that it has to get to the mycelium, and it can?t get there, if the jar is sealed.
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