|
 kickbackbro
Mr.
 
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
 Trying to be self contained and having no luck with LC's 
#11581759 - 12/04/09 04:51 AM (3 years, 5 months ago) |
|
|
I'm a noob who's done a good 200 hours or research and am fully stocked and ready to take my new hobby as far as I can. I can't seem to get to a comfort level. I initially ordered 3 separate strains of of spore syringes and thought that a good idea would be to make LC's in order to be self contained and multiply my precious little spores. I made six karo and six dex only lc's using 1TB per 800ML in a quart jar with a lid that I had put a small 1/8" hole for FAE covered in micropore tape and a self healing RVT port. I covered that with tyvek and PC'd for 20 on 15PSI. These all got knocked up with 1cc per quart. I swirled and swirled and though I had no contams, I had almost no growth. The best of the bunch were maybe around 15 percent colonized. I tried to use those to do innoc some rye berries, no luck there.
after a month of letting the LC's grow I decided to make 4 new LC's using 1TB each of light malt and dextrose per 1 quart of water. I boiled this mixture and then put it through a brita filter. I filled 5 quart jars with 3 quarts of filtered mixture and topped with a lid that I drilled a larger 1/4" FAE hole in covered again with mircropore, and they also had an RVT self healing port and tyvek. PC'd for 20 min on 15PSI and let cool. I knocked up 2 with 1cc of mutli spore and 2 I tried to clone my best LC thinking that it might colonize faster with the malt/dex nutrient ratio. 3 of them are very cloudy with only white milky, dusty sediment growth on the bottom and the third which is an LC clone has growth but is till very cloudy and it started to grow on the surface and it looks like it has pealing skin floating in it.
During this whole time period I had been trying to make WBS and Millet jars and use the original lightly colonized LC's to get some growth. Nothing, This could be for any number of reasons. Too wet, too dry, etc. But no growth here.
I decided to go back to square one and make some BRF cakes in RR's method. These I knocked up with 1cc of spore solution per 1/2pint spread over 4 holes. I was only able to do two like that but noticed that my syringe had lots of spores still in it without any liquid. I sucked up some of the same species LC from my original LC's along with some air and shook it up. I could see plenty of spores in the syringe so I used that to knock up the rest of the cakes and one WBS quart jar.
Eureka!!! all 12 cakes and the WBS are showing amazing growth. I should have just cut out the LC from day one and gone straight to grains or cakes. So there is the background and now here's my question. I have these 12 cakes about a week away from fully colonizing and a bunch of bunk LC's. I've ordered more spore syringes but want to find a TEK that will colonize grains faster than a multispore injection. I don't seem to have luck with and have read that I shouldn't even do, multispore LC's. What method would you recommend to a noob who wants to make an LC but isn't having luck. I've read about Myc h2o, agar, grain to grain and was wondering what would work best for someone like me. Or is there a "no fail" LC method anyone can recommend.
Thanks.
|
Drevrens
Stranger Than You.
Registered: 10/20/09
Posts: 326
Last seen: 4 months, 3 days
|
 Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11581800 - 12/04/09 05:01 AM (3 years, 5 months ago) |
|
|
I would also like an answer to this lol I will be watching I did up 2 syringes worth of LC's 20 Jars all Honey LC 4ml honey/100ml water. No luck at all. Not all at the same time tho did a few then did a few more then did a few more. No results but I did up 14 BRF and the first 4 are almost ready so who knows what I did wrong I know these spores are good.
--------------------
Cheers,
Drevrens
|
Randomstickynote
Registered: 07/25/09
Posts: 824
Loc: TX 
Last seen: 7 months, 5 days
|
Re: Trying to be self contained and having no luck with LC's [Re: Drevrens]
#11581835 - 12/04/09 05:13 AM (3 years, 5 months ago) |
|
|
Probably the best way that's still within your means would be to make a grain liquid culture, and inoculate your LC's with that, or just use the grain liquid culture directly.
There's plenty of tek threads on it, but basically make a jar with only a little bit of grains (or BRF/Verm) at the bottom (leaving plenty of empty space in the jar), inoculate that with spores. Once the spores have germinated, and once the grains/brf are fully colonized, shake that up to break it up, inject sterile water in that, swirl, suck the water back up. Now you have water filled with live mycelium, which should colonize a sugar LC, or more grains/PF cakes.
|
Messiah of Savants
Shaman
Registered: 07/02/08
Posts: 226
Last seen: 25 days, 22 hours
|
Re: Trying to be self contained and having no luck with LC's [Re: Randomstickynote]
#11582163 - 12/04/09 07:45 AM (3 years, 5 months ago) |
|
|
200 hours research and all the supplies huh?
Isolate a strain on agar and transfer a small piece to a sterilized LC medium.
I'd learn agar work before doing LC.
Another option is to use sterile clone tissue from one of your fruits and put it in with the LC. As long as your sterile technique is good, you should have great results. Make sure the multispore fruits you have are potent of course before you go cloning them.
Good luck!
|
 Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11582242 - 12/04/09 08:08 AM (3 years, 5 months ago) |
|
|
Quote:
Eureka!!! all 12 cakes and the WBS are showing amazing growth. I should have just cut out the LC from day one and gone straight to grains or cakes.
LC is a sideline, not the main event. It's a common misunderstanding, and it's hard to explain.
Lots of people feel that it is some sort of shortcut- it's not. Fastest way to get fruits (and spore prints) is cakes.
But now you understand. You shoot a little LC for backup, then go ahead with your jars.
Personally I think Grain LC is the way to start regular LC, better than spores. But spores work too.
--------------------
You make it all possible. Doesn't it feel good?
|
Lancaster
Rightous Oxide
Registered: 04/02/09
Posts: 419
Loc: Cape Sheridan
Last seen: 3 years, 3 days
|
Re: Trying to be self contained and having no luck with LC's [Re: Messiah of Savants]
#11582290 - 12/04/09 08:31 AM (3 years, 5 months ago) |
|
|
Quote:
suicide said:
Another option is to use sterile clone tissue from one of your fruits and put it in with the LC. As long as your sterile technique is good, you should have great results. Make sure the multispore fruits you have are potent of course before you go cloning them.
Good advice. Here's a tek.
--------------------
|
joska87
xenophobia
Registered: 09/29/09
Posts: 163
Loc: Idaho...No U Da Ho
|
Re: Trying to be self contained and having no luck with LC's [Re: Lancaster]
#11582338 - 12/04/09 08:58 AM (3 years, 5 months ago) |
|
|
I dont understand why you made sooo many LCs all you need is one tiny jar and you have enough to last forever...but anyway heres the LC tek that got me started...it worked the first time i tried it and every time since http://www.shroomery.org/forums/showflat.php/Number/5238137
this WILL work
or if you eant to make one of those grain jars LC heres the best tek fer that http://www.shroomery.org/forums/showflat.php/Number/6817707#6817707
--------------------
When I was a boy i came across two nuns makin love in cave....That typa shit wont happen in the future...
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: joska87]
#11582915 - 12/04/09 11:52 AM (3 years, 5 months ago) |
|
|
Quote:
joska87 said:
I dont understand why you made sooo many LCs all you need is one tiny jar and you have enough to last forever...but anyway heres the LC tek that got me started...it worked the first time i tried it and every time since Quote:
Well I figured being a noob and all, that my margin for error and sterile and limited experience with "clean" rooms would hinder positive results. I simply was playing the odds and trying to cheat time.
Thanks all for the feedback. I think a grain LC is the way to go for me. I have had luck with cakes and grains from multispore and this way I'll be able to tell which one is the fastest, strongest, most rhizomorphic and free of contams. Then after me gets some fruits. I'll make some twins.
Edited by kickbackbro (12/04/09 11:54 AM)
|
Breakfast Crew
Cheerios
Registered: 01/20/09
Posts: 1,985
Last seen: 1 year, 9 months
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11583091 - 12/04/09 12:28 PM (3 years, 5 months ago) |
|
|
where did you get your spores?
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: Breakfast Crew]
#11583302 - 12/04/09 01:15 PM (3 years, 5 months ago) |
|
|
used a sponsor. Strains were good and all showed fast growth straight to cakes.
|
Breakfast Crew
Cheerios
Registered: 01/20/09
Posts: 1,985
Last seen: 1 year, 9 months
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11583346 - 12/04/09 01:24 PM (3 years, 5 months ago) |
|
|
I'd say try a different brand of corn syrup for you LC. Or better yet go to a local coffee shop and get some raw sugar packets.
You can make a LC out of a fully colonized PF cake.
|
myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
|
Re: Trying to be self contained and having no luck with LC's [Re: Breakfast Crew]
#11583400 - 12/04/09 01:36 PM (3 years, 5 months ago) |
|
|
I tried raw sugar at 4g/100ml h2o and it kicked the pants off karo -great clarity,too. Grain LC rules. I used WBS, but next time I'll try well-rinsed whole brown rice for cleanliness.
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: myceleus_rex]
#11583434 - 12/04/09 01:45 PM (3 years, 5 months ago) |
|
|
So like the brown packets? Sugar in the raw? From grain LC to a sugar solution how long do you think it would take to colonize?
|
joska87
xenophobia
Registered: 09/29/09
Posts: 163
Loc: Idaho...No U Da Ho
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11583444 - 12/04/09 01:48 PM (3 years, 5 months ago) |
|
|
Quote:
kickbackbro said:
So like the brown packets? Sugar in the raw? From grain LC to a sugar solution how long do you think it would take to colonize?
if you make grain LC and have enough syringes to suck it all up you wont need to make any sugar lc, youll have enough to last you a long while.
--------------------
When I was a boy i came across two nuns makin love in cave....That typa shit wont happen in the future...
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: joska87]
#11583454 - 12/04/09 01:50 PM (3 years, 5 months ago) |
|
|
Just have about a half a dozen at the moment. Sounds like I need to get to Costco and bulk up.
|
myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11583652 - 12/04/09 02:27 PM (3 years, 5 months ago) |
|
|
Quote:
kickbackbro said:
So like the brown packets? Sugar in the raw? From grain LC to a sugar solution how long do you think it would take to colonize?
Exactly.
I was using ATL 7 spores [expensive], so I made an LC and a GLC, each with 1ml of solution. My logic was that the sugar LC would colonize faster and the GLC would be more reliable. The LC was successful and put to use in six days. The GLC jar was left alone and eventually I got some stones and culture. I've yet to try it out, but fully expect it to work. Great thing is, I still have 10ml of spores left.
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Trying to be self contained and having no luck with LC's [Re: myceleus_rex]
#11583714 - 12/04/09 02:44 PM (3 years, 5 months ago) |
|
|
Quote:
myceleus_rex said:
Quote:
kickbackbro said:
So like the brown packets? Sugar in the raw? From grain LC to a sugar solution how long do you think it would take to colonize?
Exactly.
I was using ATL 7 spores [expensive], so I made an LC and a GLC, each with 1ml of solution. My logic was that the sugar LC would colonize faster and the GLC would be more reliable. The LC was successful and put to use in six days. The GLC jar was left alone and eventually I got some stones and culture. I've yet to try it out, but fully expect it to work. Great thing is, I still have 10ml of spores left.
And that's how you do it. Easy-peasy.
--------------------
You make it all possible. Doesn't it feel good?
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: Doc_T]
#11583953 - 12/04/09 03:33 PM (3 years, 5 months ago) |
|
|
Got it. I've currently got 3 kreeper cakes in the FC. I bought some pre-packaged cakes when I started my myc garden 3 months ago before I had spores in hand. a few of them were contamed before I even got a chance to use them but 3 out of the 4 i knocked up fully colonized about a week ago and after a 24 hour dunk and roll I put them in the FC about 36 hours ago. They look great. FC is at a steady 72 degrees with humidity at 95-99 and got a 6500k 2 x 4 florescent on a 12 hour loop.
Should see some pinning any time now!
Got some more spores en route and I'll try a Grain LC, and Sugar LC with raw sugar as well as a Grain LC to Sugar LC with raw.
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11584189 - 12/04/09 04:21 PM (3 years, 5 months ago) |
|
|
Or honey or karo or dextrose or pancake syrup. It's all pretty much the same.
--------------------
You make it all possible. Doesn't it feel good?
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: Doc_T]
#11584296 - 12/04/09 04:43 PM (3 years, 5 months ago) |
|
|
But not multispore to those right? That's where I've had the trouble. I've been reading that malt/dex kicks all other LC's asses. any merit in this? I'm thinking I'll have better luck using the Grain LC method and using some of that to inoculate my cakes n' grains and some to an LC. I've tried the spore to sugar LC and clearly I suck at that.
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11584371 - 12/04/09 05:00 PM (3 years, 5 months ago) |
|
|
yes malt/dex LC's are great
heres a pic of one
but grain LC is a better way to go IMO
that way you are sure your LC is clean
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: noobieshroomie]
#11585029 - 12/04/09 06:53 PM (3 years, 5 months ago) |
|
|
and I've been using the ratio of one level tablespoon of each to a measured quart (4C of h2o) boiling and then filtering through a brita.
Does that sound right?
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11585052 - 12/04/09 06:57 PM (3 years, 5 months ago) |
|
|
i do 1 teaspoon of each to 500ml of water
then strain thru a double coffee filter
but your sounds about right
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: noobieshroomie]
#11586110 - 12/04/09 09:39 PM (3 years, 5 months ago) |
|
|
So if I have any that were filtered and are cloudy I should just dump them. I read a few posts stating that they might clear out and to just let them go for a will and then check back. But just read another one that said cloudy is probably contam.
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11586164 - 12/04/09 09:46 PM (3 years, 5 months ago) |
|
|
were they cloudy before you inoculated them?
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: noobieshroomie]
#11586294 - 12/04/09 10:03 PM (3 years, 5 months ago) |
|
|
Nah, Not so much, There was some sediment when agitated but not cloudy. I put the first batch through a brita once, and the second batch through twice. The first batch had more sediment and was a little cloudier. The second batch has only been cooking for 3 days, is not cloudy but the sediment that is on the bottom and looks to be multiplying is like tannish in color and denser than all of the pics of dex/malt LC's that I've seen on here.
Edited by kickbackbro (12/04/09 10:07 PM)
|
MasFina
Snow Shredder
Registered: 05/08/06
Posts: 788
Loc: Mountains
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11586332 - 12/04/09 10:08 PM (3 years, 5 months ago) |
|
|
kickbackbro:
See my signature LC tek
--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11586335 - 12/04/09 10:09 PM (3 years, 5 months ago) |
|
|
in my past experience once a LC is inoculated
and then turns cloudy the LC is bad
so i have just gotten into the habit
of only using clear LC;s
if you add your malt/dex to water then heat it up
it dissolves everything better
then run it thru 2 coffee filters
it always comes out clear for me
it has kind of a gold tint but no sediment
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
Rumblefishtwist
Cyber Bully
Registered: 09/27/09
Posts: 827
Loc: VA
Last seen: 1 month, 5 days
|
Re: Trying to be self contained and having no luck with LC's [Re: noobieshroomie]
#11586362 - 12/04/09 10:12 PM (3 years, 5 months ago) |
|
|
Is it possible to make a sterile LC with an already colonized BRF cake chunk mixed with sterile water?
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: Rumblefishtwist]
#11586367 - 12/04/09 10:14 PM (3 years, 5 months ago) |
|
|
take a piece of colonized cake
and put it into a sterile LC
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
Rumblefishtwist
Cyber Bully
Registered: 09/27/09
Posts: 827
Loc: VA
Last seen: 1 month, 5 days
|
Re: Trying to be self contained and having no luck with LC's [Re: noobieshroomie]
#11586390 - 12/04/09 10:18 PM (3 years, 5 months ago) |
|
|
Quote:
noobieshroomie said:
take a piece of colonized cake
and put it into a sterile LC
-noobie-
Thanks, I will try this way like the OP I am having trouble making LC's from syringe. It may be because I am using organic wildflower honey though
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: MasFina]
#11586419 - 12/04/09 10:22 PM (3 years, 5 months ago) |
|
|
Heya, Thanks, I'd already read your tek and pretty much followed that verbatim. My ratio was higher at 1T of each per Quart though, and I don't have a magnetic stir bar.
|
myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11586526 - 12/04/09 10:38 PM (3 years, 5 months ago) |
|
|
Quote:
kickbackbro said:
and I've been using the ratio of one level tablespoon of each to a measured quart (4C of h2o) boiling and then filtering through a brita.
Does that sound right?
A Brita is WAY too heavy duty. That's a carbon filter and will take out most of the nutes. Do like Noobie and use coffee filters.
|
MasFina
Snow Shredder
Registered: 05/08/06
Posts: 788
Loc: Mountains
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11586554 - 12/04/09 10:41 PM (3 years, 5 months ago) |
|
|
Quote:
kickbackbro said:
Heya, Thanks, I'd already read your tek and pretty much followed that verbatim. My ratio was higher at 1T of each per Quart though, and I don't have a magnetic stir bar.
That's a bummer. Let me see,
In your original post you said you PC'd for 20 mins .I would reduce this to 15. That should help growth because you will have less carmelization. Also, you said that you used twice as much sugar as I recommended. This was probably your biggest mistake. Keep in mind I meant 1.5tea/1.5tea per quart of water, not per quart jar. Each jar would only have 600ml of water.
Also, if you saoked the jars in b;each as i recomended you need to make sure you rinse them out very thouroughly.
Did you flame sterilize the needle before inoculation and do everything in a glove box?
did you have at least 3, 1/8" breather holes on your LC lid? This makes a big difference in speed.
After inoculation you don't wanna swirl the jar or anything, just let it sit for about 24 hours.
After that, a magnetic stirrer is best for keeping it oxygenated. otherwise you are gonna wanna swirl your jars around frequently. This might be the problem.
I wouldn't even mess with LC if I didn't have a magnetic stirrer unless I was doing Agar's grain LC tek.
Hope that helped.
The more detailed you are when describing your process, the more people can help you.
--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
Edited by MasFina (12/04/09 10:44 PM)
|
MasFina
Snow Shredder
Registered: 05/08/06
Posts: 788
Loc: Mountains
|
Re: Trying to be self contained and having no luck with LC's [Re: MasFina]
#11586589 - 12/04/09 10:47 PM (3 years, 5 months ago) |
|
|
one more thing. If you don't have a mag stirrer you are gonna have trouble with oxygenation. You should have more air in your jar to compensate. Use only 500 ml of water per jar, that should help.
--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
|
noobieshroomie
Back again
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure
|
Re: Trying to be self contained and having no luck with LC's [Re: MasFina]
#11586750 - 12/04/09 11:05 PM (3 years, 5 months ago) |
|
|
-noobie-
--------------------
AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
|
kickbackbro
Mr.
Registered: 10/28/09
Posts: 23
Last seen: 3 years, 2 months
|
Re: Trying to be self contained and having no luck with LC's [Re: MasFina]
#11587119 - 12/04/09 11:53 PM (3 years, 5 months ago) |
|
|
Thanks again, Yes I was using double your amount per quart, not per jar. I actually made 3 measured quarts and then split that into 5 jars. Noted that I'll drop the PC down to 15 min and drop my measurements down to 1.5 teas of each. Also I only have 1- 1/8" hole in the top for AE. I'll make sure to add 2 more next time and only fill the quarts halfway with the sugar solution. I did use sterile techniques for the most part. I'll put a glove box together before trying this with my new spores.
|
Steve
Stranger
Registered: 09/22/07
Posts: 442
Loc: AUS
Last seen: 10 months, 13 days
|
Re: Trying to be self contained and having no luck with LC's [Re: kickbackbro]
#11587579 - 12/05/09 01:15 AM (3 years, 5 months ago) |
|
|
I have had a few successful grows but no matter how hard I try I cannot make a LC!!!
I understand your pain!!
|
MasFina
Snow Shredder
Registered: 05/08/06
Posts: 788
Loc: Mountains
|
Re: Trying to be self contained and having no luck with LC's [Re: Steve]
#11587801 - 12/05/09 02:05 AM (3 years, 5 months ago) |
|
|
Every step in my LC tek is there for a reason. I've done it many different ways and have settled on my current technique because it gives me the best results. Print it out and check off each step as it's completed so you don't miss anything. Every step done correctly is one less thing you have to worry about and one less variable that needs to be considered when your trying to figure out what went wrong.
Mushroom cultivation is all about the details. Laziness is not rewarded.
You are also fighting millions of invisible micro enemies. You have to be crafty to get the upper hand.
--------------------
A Good Substrate: Poo With Extras
Good Liquid Culture, Step by Step
Timer Modification
PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping
Edited by MasFina (12/05/09 02:07 AM)
|
ChaostoOrder
Lakota Peji Wicasa,
Registered: 08/01/09
Posts: 474
Last seen: 1 year, 7 months
|
Re: Trying to be self contained and having no luck with LC's [Re: MasFina]
#11587913 - 12/05/09 02:38 AM (3 years, 5 months ago) |
|
|
If you want to go extra simple, wheat pasta tek worked well for me....Get organic wheat pasta, boil according to directions or not....strain juice into a jar then let sit over night so everything settles, make some alfredo if you want your choice...after it set over night siphon off 20 ML into your LC jar and add 250ml of purified water....PC for an hour....then let sit for 4 to six hours so your not knocking up a hot jar....then knock up ....works!!!!
--------------------
Too weird to live, to rare to die....-Hunter S. Thompson
Hillbilly prints to trade pm me!! 
Tatanka Oyate
|
rottypup
Stranger
Registered: 09/26/09
Posts: 43
Last seen: 3 years, 3 months
|
Re: Trying to be self contained and having no luck with LC's [Re: ChaostoOrder]
#11588009 - 12/05/09 03:02 AM (3 years, 5 months ago) |
|
|
The LC you made and didn't see results from... maybe you didn't wait long enough.
Here's where I'm coming from: I'm a newbie and made 2 LC--one from honey and one from karo. They both colonized (but pathetically)--just tiny amount of mycilium. So I innoc'd a few jars and saw no results within a week. It didn't look promising, and then, 2 1/2 weeks later--there's the mycilium growth!
So basically, when you make a LC and the mycilium growth looks puny, it will still work. It just takes a lot longer!
(Just thought I'd share cause everyone on this site talks about their LC colonizing jars rapidly, but I haven't had the same fast results--yet..)
|
|
Images only 
Threaded
Previous
Index
Next
Shop for: 
Edit 
Reply 
