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 Fungus Amongus
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 I once was contam'ed, but now, I'm healthy?   1
#10188616 - 04/18/09 10:26 AM (4 years, 1 month ago) |
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Hi all!
First time posting on this site and after trying to peruse the plethora of rules and guidelines to posting, I think I'm ready.
I bought one of those EZ-GRO kits about a month ago. I wont get into what made me buy it too much because thats a whole 'nother posting but lets just say.. the simplicity was its biggest appeal.
I inoculated the rye berry substrate and within 4 days had mycelium growing. there was evidence of excellent rhizomorphic (sp?) growth and soon another small patch started to develop.
I pretty much went backwards every step of the way on this but I live in FL and its hard to keep a decent temp for growing indoors cause I always have the AC on so I dont bake-- thus lowering humidity and temp indoors. I had the substrate bag on my window sill with the blinds shut because it constantly stays about 80 degrees there.
One day I looked and noticed a greyish/greenish tinge on just 1 grain of rye berry. I did research on the site and with 97% certainty I believe it was 'trich'. the bag has a filter on it but lord knows what kind or if its effective.
I left it alone as I conducted my research over 24 hours. When I came back and inspected again...the green/grey tint had spread to roughly 5 rye berries all in the same location and all away from the mycelium.
I immediately went into surgery mode. I made a sterilized environment, cut open the substrate bag and carefully removed the bad kernels. I then used another tool to pull the 2 chunks of mycelium out of the bag. 1 was exactly half the size of a baseball and one was half the size of a ping pong ball. Total inoculation was about 34% of substrate bag.
In nearly one motion the mycelium went from the substrate bag into the growing bag filled with kow-a-nite compost. I sealed the bag and broke up the chunks a little bit..but because I was scared I just ruined the whole thing (because they didn't fully colonize) that I figured i'd leave large chunks in an effort to give a better chance of growth.
I placed this bag in the cabinet above my fridge and left it alone.
Now 4 days later I have LOTS of rhizo growth all throughout the compost. I did not tamp the compost down or shape it into a block so there are peaks and valleys and large boulders of compost and deep rifts...ropey, strandy mycelium is growing vibrantly throughout and at a much fast pace than what it did in the rye berries in a warmer environment.
my question is this:
Can I shake up and re-distribute the mycelium at this point to colonize more of the compost? half the bag is untouched as the mycelium hasn't made its way over there though it looks like it would...
or it the mycelium now trying to fruit itself and shaking and breaking it up could kill it?
should I tamp the bag down?
the bag is made for fruiting. it should be kept in 12 hours light 12 hours dark with air exchange and proper humidity...right now its in total darkness with the exception of when I pull it out and gently press on the bag to force air out and expand it to bring in fresh air. Will it only fruit after I give it the proper amount of light or will it begin to fruit regardless?
I pretty much went backwards every step of the way on this but I live in FL and its hard to keep a decent temp for growing indoors cause I always have the AC on so I dont bake-- thus lowering humidity and temp indoors. I had the substrate bag on my window sill with the blinds shut because it constantly stays about 80 degrees there.
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RombaZomba
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 Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10189023 - 04/18/09 12:20 PM (4 years, 1 month ago) |
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You only shake once and when it is less than halfway finished colonizing.
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Fungus Amongus
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Re: I once was contam'ed, but now, I'm healthy? [Re: RombaZomba]
#10189824 - 04/18/09 02:46 PM (4 years, 1 month ago) |
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Thanks a bunch. should I attempt to fruit before full colonization? I've heard something about overlay? Should I put some more compost on top of the fully colonized bag like a casing layer or is that not needed?
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Fungus Amongus
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Registered: 04/12/09
Posts: 232
Loc: FL
Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10195722 - 04/19/09 04:30 PM (4 years, 1 month ago) |
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well I have shaken the bag of compost and mixed the mycelium really well. I see some growth around the edges of the bits of mycelium but its too soon so see real noticeable difference. I have a feeling although i have it in the cupboard above my fridge, the RH is low. there is moisture all over the inside of the bag but there used to be much more.
We'll see what happens...how long should I give it before fruiting I wonder?
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Fungus Amongus
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Registered: 04/12/09
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Loc: FL
Last seen: 2 months, 19 days
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 Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10199864 - 04/20/09 08:06 AM (4 years, 1 month ago) |
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lots of growth in 24 hours! entire chunks of compost on the top are completely colonized. wondering when I should start giving these guys light...? any suggestions?
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 FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,615
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10212488 - 04/22/09 10:20 AM (4 years, 29 days ago) |
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I suggest reading up on "Pinning Triggers" ...theres an order of importance: (please correct me if I'm wrong)(I'm going off of memory)
1. Running out of food
2. Decrease in CO2
3. Tempature drop
4. light
I say that, to say this, the first thing you wanna be sure of before "Birthing" (so to speak) is that its FULLY COLONIZED; which is generally a week after you THINK it is...
As far as I know; Light doesn't really matter if the other things havn't happened...
-Fellowgrower
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Fungus Amongus
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Registered: 04/12/09
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Loc: FL
Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: FellowGrower]
#10218453 - 04/23/09 08:41 AM (4 years, 28 days ago) |
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Thanks fellow grower.
So far the mycelium has taken off and grown like crazy in sections.
I'm thinking I should shake it one more time to get a real even distribution?
I have taken pictures but the quality sucks when trying to shoot through a plastic bag with moisture and cow poop on the sides.
The mycelium is very strand-like...with stalks of pure mycelium reaching up about 2-3mm above the substrate and forming what looks like hyphial knots on the tops of the stalks. DEF not pinning mushrooms I dont think but they resemble super tiny aborts.
Anyway they are still in darkness and there is still plenty of moisture. and the susbstrate is about 34% colonized.
Would anyone suggest letting the entire bag of compost colonize and then birth the whole 3 pound chunk in a fruiting chamber or should I fruit them inside the bag as advised by homestead?
Do the fruits grow from the nutrients in the initial substrate of rye berries or does the compost act as a supplier of nutrients?
I can use all the advice I can get as I desperately dont want to ruin my first time growing.
Thanks for the support all and keep the advice flowing! pictures to be posted soon!
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FellowGrower
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Posts: 1,615
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10222611 - 04/23/09 10:49 PM (4 years, 28 days ago) |
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Hey, Its all about giving back! you can pay me back for my advice by continuing to come here (once you get some grows under your belt) and help out the new guy.
Now...Does it look like this (below)
Or like this...
I would suggest NOT breaking it up REASON: It sets it back, and gives contams more of a chance to get a foothold.
Fruiting in the bag? IDK? never seen a invitro bag "Blow up" w/ fruits...but its alot "Less Risky" so, that's gonna have to be up to you...Its gonna pull nutes from both.
If you dont' wanna fuck it up; stop playing with it so much... You ever seen that Chris Farley movie "Tommyboy" ...Remember when hes got that roll at the restaurant and he's like "I love it" and Touch it" and snuggle' and then starts rippen the shit everywhere throwing it Yelling... See? it's like that. the more you "Fondel it" the more likely it goes south. I know its hard, but just forget about it for a few days...then when you come back...you're like... 
As stated previously, the darkness doesn't matter if its not fully colonized... see? theyre in ORDER (for a reason)
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Fungus Amongus
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Registered: 04/12/09
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Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: FellowGrower]
#10223797 - 04/24/09 02:23 AM (4 years, 28 days ago) |
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It looks like this...
I included a picture of the bag that its contained in
The brown spots are some rye berries.
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FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,615
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10224478 - 04/24/09 06:49 AM (4 years, 27 days ago) |
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If you can get that sticker off it'd be better; but, don't comprimise the bag if you can't.
And as far as I can tell all is well.
...just needs Time and TLC; and, all you need is Patientce and a plan!
Do you know how to "Case it?" ...have you read Hyphaes "Pinning Strategy" CLICK ON THIS...
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RogerRabbit
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Re: I once was contam'ed, but now, I'm healthy? [Re: FellowGrower]
#10228916 - 04/25/09 12:04 AM (4 years, 27 days ago) |
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You should just watch and when it's fully colonized, open the bag up to get some fresh air in there every day if you're going to be fruiting in the bag. That amount of compost was intended to use the whole amount of rye berries for inoculation, so with such a small inoculant, it might not fully colonize before the compost molds.
I can tell the compost is too wet. Nothing should be sticking to the sides of the bag. At this point you don't want to open it because fresh air is a pinning trigger and could screw up the process. Next time, before you inoculate compost or manure, squeeze out nearly all the water you can by hand wringing. Good luck.
RR
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Fungus Amongus
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Registered: 04/12/09
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Loc: FL
Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: RogerRabbit]
#10231714 - 04/25/09 05:21 PM (4 years, 26 days ago) |
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Thanks RR
when I got the kit, it had that moisture in it. I had no idea it was too much since this was my first time and the kit promises ease of use.
originally after I broke up the rye berries in there, there was no pieces of crap on the side of the bag. That happened after I shook up the mycelium that started growing.
I haven't checked it in a day and a half, but so far I haven't noticed any abnormal discoloration.
I would like to dunk the fully colonized manure and then case it and fruit in a terrarium.
it that too ambitious? Can it be done? should I leave and fruit in the bag? what about taking the cake out and just fruiting that in a chamber?
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FellowGrower
Fungal Freedom Fighter
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Posts: 1,615
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10232037 - 04/25/09 06:24 PM (4 years, 26 days ago) |
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Hey, does that pic I posted above look "Off" to anybody? something aint right...no rhizos, and no smell, do I gotta bag fulla COBWEB?
WTF? I'm asking about the smell b/c, its supposed to smell good, but this bag has no smell
...am I gonna have to start my own thread about it?
-damnit-
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Fungus Amongus
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Registered: 04/12/09
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Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: FellowGrower]
#10232382 - 04/25/09 07:46 PM (4 years, 26 days ago) |
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Yeah im obviously no expert. It looks like myc but its a bit blurry. I assume you're talking about the bottom pic? Looks like cobwebish but I've seen other pics of it and yours doesn't look like them...
but then again I can't see the white too clearly in that pic...
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Fungus Amongus
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Posts: 232
Loc: FL
Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10265929 - 04/30/09 11:58 PM (4 years, 21 days ago) |
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I have knots forming on the most colonized chunk of manure and what I believe are pin heads as well. the larger threads of mycelium formed what looked like a tree structure with a frothy white foam-like mycelium for the tree top. from that, the pins are jutting out about 1mm.
Should I start to fruit them or leave the bag in darkness still? the bag is about 50% colonized
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FellowGrower
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10267473 - 05/01/09 09:52 AM (4 years, 20 days ago) |
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My GUESS would be that if its pinning and there's still un-eaten sub in there, the un-eaten part is contamed...(and not gonna colonize) so...??? 
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Fungus Amongus
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Last seen: 2 months, 19 days
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Re: I once was contam'ed, but now, I'm healthy? [Re: FellowGrower]
#10267604 - 05/01/09 10:58 AM (4 years, 20 days ago) |
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well the deal is, the particular chunk of manure got colonized very quickly. The rest of the bag is colonizing and its growing just fine..but this one particular chunk went really fast and thus I was asking about RE-SHAKING the bag to redistribute the mycelium.
I dont think theres any contams because its growing more and more mycelium in new places all the time...its just that one chunk got fully colonized hardcore and has seemingly begun to pin. The pins have had no growth and they seem to have stalled at 1mm.
Can I shake the bag at this point or is that really playing with fire? I just want to fully colonize the bag quickly and as RR said, there was never enough inoculate to colonize the manure properly. No mold has formed anywhere and the air filter patch when the bag is squeezed smells like mushrooms and mushrooms only 
Let me know if shaking at this point isn't such a bad idea or if it is. Let me know if you need pictures of the current growth to determine what your suggestions would be.
Again this is my first time growing and my main goal is to get
a) at least 1 mushroom to make a spore print and do all this again with PF TEK
b) enough mushrooms for me to trip...about an 8th.
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Fungus Amongus
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10283227 - 05/04/09 01:35 PM (4 years, 17 days ago) |
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UPDATE.
I have taken the bag out of the cabinet above my refridge as the temp inside the cabinet has dropped since we have begun to use the AC more and more.
I transferred the bag to a large insulated hard plastic cooler and left it out on my porch. Temp inside reaches about 88 degrees and mycelium has rapidly grown with only 12 hours in the cooler.
The cooler dropped its temp to about 68 degrees overnight so I will take it inside from now on in hopes to keep the daytime heat insulated better overnight.
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Fungus Amongus
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Re: I once was contam'ed, but now, I'm healthy? [Re: Fungus Amongus]
#10289308 - 05/05/09 11:53 AM (4 years, 16 days ago) |
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Holy fuck. I took the bag inside for the night in order to keep the temp right....well this morning i go and check it and fuck me running there are about 50 white dots all over the colonized chunks and 2 actual pinheads. about 6mm in height with a 1.5mm cap diameter. I have now placed the bag in indirect sunlight to allow for proper fruiting.
I am hesitant to follow RRs instructions because I dont want to open the bag up if there is so much uncolonized manure. Any suggestions? Currently to flush the air, I press down on the bag as far as I can without touching the compost and then expand the bag to force out CO2 through the filter patch and take in O2 the same way.
this will work for now, but as the shrooms grow, i wont be able to squeeze as much air out.
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Fungus Amongus
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ok here are some pics of my current situation...
I opened the bag to fan some fresh air in. doesn't smell like anything funky...I guess it smells like mushrooms?
any advice at this point?
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FellowGrower
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Posts: 1,615
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dude, I trippen right now and that Pic just BLOWS MY MIND! is it still in the bag? the dimensions of that pic are rediculous?
white dots are good, though I dont see em?
k,
Yeah, uh... Do that... whatever man. IDK what to tell you?
wish I did,(for some reason)
k BYE, TTYL,
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Fungus Amongus
Spore Herder
Registered: 04/12/09
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First Flush...1 mushroom
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FellowGrower
Fungal Freedom Fighter
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Posts: 1,615
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    ....
"Keep coming back..."
pft- better than nothin!
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Fungus Amongus
Spore Herder
Registered: 04/12/09
Posts: 232
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Last seen: 2 months, 19 days
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WELL I came back and this is what I see...
Now I have two mushrooms. I took the caps off both of them and have placed them on a piece of tin foil with a shot glass over them to get a spore print. Its been 24 hours and nothing yet. Did i pull the cap off too early? did it need to expand more? Everyone said to pull the shroom as soon as the veil breaks to avoid sporing all over... what gives? do I need to let them dry more before covering with glass? Do i need to wait 2 days?
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Fungus Amongus
Spore Herder
Registered: 04/12/09
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nevermind. i got it. folded up the tin foil and put it in a zip lock and into the fridge.
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FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,615
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HAY!...Its ya burfday, no for real doe, not foe play play, grew some shrooms yo, oh shit now...
Good Job! ...5 shrooms for you!
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Fungus Amongus
Spore Herder
Registered: 04/12/09
Posts: 232
Loc: FL
Last seen: 2 months, 19 days
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yeah i have a feeling its all going to shit. No pun intended.
picked all those shrooms and split them with my friend and ate them.
2.5g semi-dried each. NOTHING!
sooo fucking pissed that i blew my harvest for NOTHING.
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